Mechanisms of Cisplatin-Induced Apoptosis and of Cisplatin Sensitivity: Potential of BIN1 to Act as a Potent Predictor of Cisplatin Sensitivity in Gastric Cancer Treatment

Tanida, Satoshi; Mizoshita, Tsutomu; Ozeki, Keiji; Tsukamoto, Hironobu; Kamiya, Toshio; Kataoka, Hiromi; Sakamuro, Daitoku; Joh, Takashi

doi:10.1155/2012/862879

Cited by 88 publications

(76 citation statements)

References 34 publications

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“…Previous reports showed that Cisplatin stimulated apoptosis via intrinsic and extrinsic pathways [31] and cFLIP knockdown increased sensitivity to Cisplatin [17]. CoCl 2 is a chemical inducer of hypoxia-inducible factor-1 (HIF1) [32].…”

Section: Discussionmentioning

confidence: 99%

Cflarb Complemented the Function of Cflara to Allow Cflara Knock out Zebrafish To Normal Development

Huh¹,

Hwang²,

Koppula³

et al. 2017

Cell Dev Biol

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Cellular FLICE-inhibitory protein (cFLIP, cflara) is a regulator of death receptor (DR)-induced apoptosis and NF-κB activation. cFLIP is known to prevent activation of the caspase cascade by binding to FADD/caspase-8. Up-regulated cFLIP has been identified in many tumor types, and therefore restoring apoptosis by silencing cFLIP may be one of the more potent strategies in cancer therapeutics. The zebrafish cFLIP gene, cflara, has 2 death effector domains (DEDs) and a single caspase-like domain. Expression of cflara was detected in the zebrafish embryo by RT-PCR and wholemount in situ hybridization. To study the in vivo function of cflara, we generated a cflara knockout mutant zebrafish using transcription activator-like effector nucleases (TALENs). Frame shift mutation is caused by a 10 bp deletion in the first DED domain. By inbreeding the F1 generation, a homozygous mutant fish was produced and confirmed by PCR. Knockout of cflara leads to abnormal heart development and embryonic lethality in mice. However, mutant zebrafish did not show any differences from wild type in heartbeat rate, survival rate or development. Zebrafish have analogues of both cflara and cflarb. Quantitative PCR showed that cflarb mRNA levels of mutant zebrafish were higher than those in the wild type. In a chemical exposure experiment, mutant zebrafish larvae showed a longer survival rate compared with wild type after CoCl 2 treatment. However, no significant difference was observed from cisplatin treatment. This data suggests that cflarb may contribute to normal development and causes a difference in chemical resistance.

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Section: Discussionmentioning

confidence: 99%

Cflarb Complemented the Function of Cflara to Allow Cflara Knock out Zebrafish To Normal Development

Huh¹,

Hwang²,

Koppula³

et al. 2017

Cell Dev Biol

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“…This p53 gene stimulates expression of Bcl-2 family including Bax, and can bind to one or more anti-apoptotic proteins in mitochondria, such as Bcl-XL (Chipuk, et al, 2003;Marsden, et al, 2002). On the other hand, cisplatin also plays a role in mediating the activation of the p53 protein, which is followed by the occurrence of DNA damage (Tanida, et al, 2012), so the mutation of p53 gene on T47D cells can inhibit cisplatin to induce apoptosis. However, previous studies had proved that the mechanism of cisplatin to induce apoptosis was by causing downregulation of Bcl-2 gene on MCF 7 cell lines (Thomadaki and Scorilas 2007) and T47D cell lines (Mokhtari, et al, 2012).…”

Section: (D) (E)mentioning

confidence: 99%

Cytotoxic and Apoptotic-inducing Effect of Fraction Containing Brazilein from Caesalpinia sappan L. and Cisplatin on T47D Cell Lines

Tirtanirmala¹,

Novarina²,

Utomo³

et al. 2017

IJCC

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Anticancer activity of secang's heartwood (Caesalpinia sappan L.) is based on its main compound: brazilin and brazilein. Brazilin, brazilein, and other compounds such as caesalpiniaphenol can affect proteins that have a role in apoptosis. In this study, we observed cytotoxic activity of fraction containing brazilein (FCB) alone or in combination with chemotherapeutic agent, cisplatin and the ability of the combination to induce apoptosis in T47D breast cancer cell lines. Cytotoxicity assay was determined using MTT assay, whereas the detection apoptosis induction was conducted using flow cytometry using Annexin-V and propidium iodide. FCB and cisplatin showed cytotoxic effect on T47D cells with IC50 value of 68 µg/mL and 16 µM, respectively. Combination of FCB and cisplatin result synergistic combination at the concentration ratio of 1/2 IC50 with CI value of 0.66. Its combination also able to induce apoptosis on T47D cell population 13% larger than the single treatment. Based on this study, we conclude that FCB is able to enhance the cytotoxic effects of cisplatin by inducing apoptosis.

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“…Also, Amphiphysin 2 supports T-cell mediated immune surveillance against tumors. The mechanism by which Amphiphysin 2 promotes tumor suppression is unknown, though it has been shown to promote apoptosis in cancer cells via activation of Bax 90 and is known to promote DNA repair by binding and inhibiting poly (ADP-ribose) polymerase (PARP1), a key component in base excision repair pathway via its BAR domain. 89 …”

Section: Cell Cycle Regulationmentioning

confidence: 99%

Dynamins and BAR Proteins-Safeguards against Cancer

Sundborger

Hinshaw

2015

Crit Rev Oncog

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Dynamins and BAR proteins are crucial in a wide variety of cellular processes for their ability to mediate membrane remodeling, such as membrane curvature and membrane fission and fusion. In this review, we highlight dynamins and BAR proteins and the cellular mechanisms that are involved in the initiation and progression of cancer. We specifically discuss the roles of these proteins in endocytosis, endo-lysosomal trafficking, autophagy, and apoptosis as these processes are all tightly linked to membrane remodeling and cancer.

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Mechanisms of Cisplatin-Induced Apoptosis and of Cisplatin Sensitivity: Potential of BIN1 to Act as a Potent Predictor of Cisplatin Sensitivity in Gastric Cancer Treatment

Cited by 88 publications

References 34 publications

Cflarb Complemented the Function of Cflara to Allow Cflara Knock out Zebrafish To Normal Development

Cflarb Complemented the Function of Cflara to Allow Cflara Knock out Zebrafish To Normal Development

Cytotoxic and Apoptotic-inducing Effect of Fraction Containing Brazilein from Caesalpinia sappan L. and Cisplatin on T47D Cell Lines

Dynamins and BAR Proteins-Safeguards against Cancer

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