Mechanisms of EDDHA effects on the promotion of floral induction in the long-day plantLemna minor (L.)

Krajnčič, Božidar; Nemec, Jože

doi:10.1078/0176-1617-00794

Cited by 7 publications

(1 citation statement)

References 18 publications

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“…We successfully induced mature flowers at high frequencies for L. gibba clones 7741 and 5504, respectively. No flower induction was observed, under any of the conditions tested, in L. minor 7210, which suggested that different duckweed species responded differently to SA induction and may need different methods to induce flowering, e.g., in the case of L. minor , ethylenediamine-N,N’-bis (2-hydroxyphenylacetic acid) (EDDHA) is a successful flower-inducer 40 . Although no flowering was induced in L. minor 7210 by SA, its growth rate was reduced significantly, which indicated that growth inhibition was not a result of flowering.…”

Section: Discussionmentioning

confidence: 99%

Flower induction, microscope-aided cross-pollination, and seed production in the duckweed Lemna gibba with discovery of a male-sterile clone

Huang

Han

et al. 2017

Sci Rep

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Duckweed species have a great potential to develop into fast-growing crops for water remediation and bioenergy production. Seed production and utilization of hybrid vigour are essential steps in this process. However, even in the extensively-studied duckweed species, Lemna gibba, flower primordia were often aborted prior to maturation. Salicylic acid (SA) and agar solidification of the medium promoted flower maturation and resulted in high flowering rates in L. gibba 7741 and 5504. Artificial cross-pollination between individuals of L. gibba 7741 yielded seeds at high frequencies unlike that in L. gibba 5504. In contrast to clone 7741, the anthers of 5504 did not dehisce upon maturation, its artificially released pollen grains had pineapple-like exine with tilted spines. These pollens were not stained by 2,5-diphenylmonotetrazoliumbromide (MTT) and failed to germinate. Therefore, clone 5504 is male sterile and has potential application with respect to hybrid vigour. Moreover, pollination of flowers of 5504 with 7741 pollen grains resulted in intraspecific hybrid seeds, which was confirmed by inter-simple sequence repeat (ISSR) markers. These hybrid seeds germinated at a high frequency, forming new clones.

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Section: Discussionmentioning

confidence: 99%