Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

Ouboter, Heleen T.; Mesman, Rob; Sleutels, Tom; Postma, Jelle; Wissink, Martijn; Jetten, Mike S. M.; Ter Heijne, Annemiek; Berben, Tom; Welte, Cornelia U.

doi:10.1038/s41467-024-45758-2

Cited by 11 publications

(1 citation statement)

References 72 publications

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“…However the Borg proteins identified are not known to transfer electrons or play a role in metabolism 34 . Subsequently, it was noted that Methanoperedens genomes encode nanowire assembly genes homologous to cytochrome OmcZ, which is processed by OzpA ( O mc Z p rotease), and prolyl isomerase, and EET has been confirmed experimentally 31 , 35 . Of 111 cytochromes, OmcZ is the only nanowire-forming outer-surface cytochrome essential for long-range (>10 µm) EET by microbial communities 31 , 32 .…”

Section: Resultsmentioning

confidence: 98%

Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires

Schoelmerich,

Ly,

West-Roberts

et al. 2024

Nat Commun

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Borgs are huge extrachromosomal elements (ECE) of anaerobic methane-consuming “Candidatus Methanoperedens” archaea. Here, we used nanopore sequencing to validate published complete genomes curated from short reads and to reconstruct new genomes. 13 complete and four near-complete linear genomes share 40 genes that define a largely syntenous genome backbone. We use these conserved genes to identify new Borgs from peatland soil and to delineate Borg phylogeny, revealing two major clades. Remarkably, Borg genes encoding nanowire-like electron-transferring cytochromes and cell surface proteins are more highly expressed than those of host Methanoperedens, indicating that Borgs augment the Methanoperedens activity in situ. We reconstructed the first complete 4.00 Mbp genome for a Methanoperedens that is inferred to be a Borg host and predicted its methylation motifs, which differ from pervasive TC and CC methylation motifs of the Borgs. Thus, methylation may enable Methanoperedens to distinguish their genomes from those of Borgs. Very high Borg to Methanoperedens ratios and structural predictions suggest that Borgs may be capable of encapsulation. The findings clearly define Borgs as a distinct class of ECE with shared genomic signatures, establish their diversification from a common ancestor with genetic inheritance, and raise the possibility of periodic existence outside of host cells.

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Section: Resultsmentioning

confidence: 98%

Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires

Schoelmerich,

Ly,

West-Roberts

et al. 2024

Nat Commun

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Mechanisms of microbial co-aggregation in mixed anaerobic cultures

Doloman,

Sousa

2024

Appl Microbiol Biotechnol

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Co-aggregation of anaerobic microorganisms into suspended microbial biofilms (aggregates) serves ecological and biotechnological functions. Tightly packed aggregates of metabolically interdependent bacteria and archaea play key roles in cycling of carbon and nitrogen. Additionally, in biotechnological applications, such as wastewater treatment, microbial aggregates provide a complete metabolic network to convert complex organic material. Currently, experimental data explaining the mechanisms behind microbial co-aggregation in anoxic environments is scarce and scattered across the literature. To what extent does this process resemble co-aggregation in aerobic environments? Does the limited availability of terminal electron acceptors drive mutualistic microbial relationships, contrary to the commensal relationships observed in oxygen-rich environments? And do co-aggregating bacteria and archaea, which depend on each other to harvest the bare minimum Gibbs energy from energy-poor substrates, use similar cellular mechanisms as those used by pathogenic bacteria that form biofilms? Here, we provide an overview of the current understanding of why and how mixed anaerobic microbial communities co-aggregate and discuss potential future scientific advancements that could improve the study of anaerobic suspended aggregates. Key points • Metabolic dependency promotes aggregation of anaerobic bacteria and archaea • Flagella, pili, and adhesins play a role in the formation of anaerobic aggregates • Cyclic di-GMP/AMP signaling may trigger the polysaccharides production in anaerobes

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Comparing the effects and mechanisms of granular activated carbon and magnetite nanoparticles in dry anaerobic digestion: Focusing on microbial electron transfer and metagenomic evidence

Wang,

Cheng,

Zhang

et al. 2024

Chemical Engineering Journal

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Mechanisms of extracellular electron transfer in anaerobic methanotrophic archaea

Cited by 11 publications

References 72 publications

Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires

Borg extrachromosomal elements of methane-oxidizing archaea have conserved and expressed genetic repertoires

Mechanisms of microbial co-aggregation in mixed anaerobic cultures

Comparing the effects and mechanisms of granular activated carbon and magnetite nanoparticles in dry anaerobic digestion: Focusing on microbial electron transfer and metagenomic evidence

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