Mechanisms of HBO-Induced Vascular Functional Changes in Diabetic Animal Models

Jukić, Ivana; Mišir, Mihael; Mihalj, Martina; Mihaljević, Zrinka; SanelaUnfirer,; Kibel, Dijana; Kibel, Aleksandar

doi:10.5772/intechopen.76569

Cited by 3 publications

(2 citation statements)

References 103 publications

(119 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The exact mechanisms by which HBO achieves its beneficial angiogenic action are not completely investigated. However, it was found that HBOT stimulates the release of EPCs and increases the production of angiogenic factors such as vascular endothelial growth factor (VEGF) [ 7 , 9 , 44 ]. furthermore, a genome-wide microarray analysis was conducted by Godman et al [ 45 ] on human microvascular endothelial cells subjected to HBOT.…”

Section: Discussionmentioning

confidence: 99%

Hyperbaric oxygen therapy efficacy on mandibular defect regeneration in rats with diabetes mellitus: an animal study

et al. 2023

View full text Add to dashboard Cite

Background This study aimed to investigate the influence of hyperbaric oxygen therapy on mandibular critical-sized defect regeneration in rats with experimentally induced type I diabetes mellitus. Restoration of large osseous defects in an impaired osteogenic condition such as diabetes mellitus is a challenging task in clinical practice. Therefore, investigating adjunctive therapies to accelerate the regeneration of such defects is crucial. Materials and methods Sixteen albino rats were divided into two groups (n = 8/group). To induce diabetes mellitus, a single streptozotocin dosage was injected. Critical-sized defects were created in the right posterior mandibles and filled with beta-tricalcium phosphate graft. The study group was subjected to 90-min sessions of hyperbaric oxygen at 2.4 ATA, for 5 consecutive days per week. Euthanasia was carried out after 3 weeks of therapy. Bone regeneration was examined histologically and histomorphometrically. Angiogenesis was assessed by immunohistochemistry against vascular endothelial progenitor cell marker (CD34) and the microvessel density was calculated. Results Exposure of diabetic animals to hyperbaric oxygen resulted in superior bone regeneration and increased endothelial cell proliferation, which were revealed histologically and immunohistochemically, respectively. These results were confirmed by histomorphometric analysis which disclosed a higher percentage of new bone surface area and microvessel density in the study group. Conclusions Hyperbaric oxygen has a beneficial effect on bone regenerative capacity, qualitatively and quantitively, as well as the ability to stimulate angiogenesis.

show abstract

Section: Discussionmentioning

confidence: 99%

Hyperbaric oxygen therapy efficacy on mandibular defect regeneration in rats with diabetes mellitus: an animal study

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Endothelial cells metabolize arachidonic acid (AA) via CYP450 enzymes (ω-hydroxylase and epoxygenase), via cyclooxygenases and 5-lipooxygenase, and by non-enzymatic degradation of arachidonic acid in the presence of free radicals [ 10 ]. Epoxygenases are members of cytochrome P450 family of enzymes, which in the endothelial cell produce four epoxyeicosatrienoic acid (EET) isomers, of which 14,15-EET and 11,12-EET are the major active vasodilator metabolites [ 10 , 11 , 12 , 13 , 14 , 15 ]. ω-hydroxylase, in smooth muscle cells, promotes the production of 20-hydroxy-eicosatrienoic acid (20-HETE), which is a vasoconstrictor [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Arachidonic Acid Metabolites of CYP450 Enzymes and HIF-1α Modulate Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats under Acute and Intermittent Hyperbaric Oxygenation

Mihaljević

Matić

Stupin

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

Acetylcholine-induced vasorelaxation (AChIR) and responses to reduced pO2 (hypoxia-induced relaxation (HIR), 0% O2) were assessed in vitro in aortic rings of healthy male Sprague-Dawley rats (N = 252) under hyperbaric (HBO2) protocols. The studied groups consisted of the CTRL group (untreated); the A-HBO2 group (single HBO2; 120 min of 100% O2 at 2.0 bars); the 24H-HBO2 group (examined 24 h after single exposure) and the 4D-HBO2 group (four consecutive days of single HBO2). AChIR, sensitivity to ACh and iNOS expression were decreased in the A-HBO2 group. HIR was prostanoid- and epoxyeicosatrienoic acid (EET)-mediated. HIF-1α expression was increased in the 24H-HBO2 and 4D-HBO2 groups. LW6 (HIF-1α inhibitor) decreased HIR in the 24H-HBO2 group. HBO2 affected the expression of COX-1 and COX-2. CYP2c11 expression was elevated in the 24H-HBO2 and 4D-HBO2 groups. Concentrations of arachidonic acid (AA) metabolites 14(15)-DiHET, 11(12)-DiHET and 8(9)-DiHET were increased in A-HBO2 and 24H-HBO2. An increased concentration of 8(9)-EET was observed in the A-HBO2 and 24h-HBO2 groups vs. the CTRL and 4D-HBO2 groups, and an increased concentration of 5(6)-DiHET was observed in the 24H-HBO2 group vs. the 4D-HBO2 group. The 20-HETE concentration was increased in the A-HBO2 group. All were determined by LC-MS/MS of the aorta. The results show that AChIR in all groups is mostly NO-dependent. HIR is undoubtedly mediated by the CYP450 enzymes’ metabolites of AA, whereas HIF-1α contributes to restored HIR. Vasoconstrictor metabolites of CYP450 enzymes contribute to attenuated AChIR and HIR in A-HBO2.

show abstract

The influence of low- intensity laser irradiation versus hyperbaric oxygen therapy on transcutaneous oxygen tension in chronic diabetic foot ulcers: a controlled randomized trial

Wadee

Aref

Nassar

et al. 2021

J Diabetes Metab Disord

View full text Add to dashboard Cite

Mechanisms of HBO-Induced Vascular Functional Changes in Diabetic Animal Models

Cited by 3 publications

References 103 publications

Hyperbaric oxygen therapy efficacy on mandibular defect regeneration in rats with diabetes mellitus: an animal study

Hyperbaric oxygen therapy efficacy on mandibular defect regeneration in rats with diabetes mellitus: an animal study

Arachidonic Acid Metabolites of CYP450 Enzymes and HIF-1α Modulate Endothelium-Dependent Vasorelaxation in Sprague-Dawley Rats under Acute and Intermittent Hyperbaric Oxygenation

The influence of low- intensity laser irradiation versus hyperbaric oxygen therapy on transcutaneous oxygen tension in chronic diabetic foot ulcers: a controlled randomized trial

Contact Info

Product

Resources

About