Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

Arnhold, Jürgen; Mueller, Sebastian; Arnold, Katrin; Sonntag, K.

doi:10.1002/bio.1170080604

Cited by 50 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Then, the luminol-dependent chemiluminescence is considered to be due to the reaction of H 2 O 2 with luminol oxidized by ClO Ϫ . 13,[18][19][20][21][22] After the addition of H 2 O 2 and NaClO to the mixture of catalase, DTPA, and luminol, high CL intensity was observed with increasing concentrations of H 2 O 2 as shown in Fig. 1 where the Michaelis-Menten type of equation (CLϭ CL max · C/(K m ϩC), CϭH 2 O 2 concentration) was fitted well with the observed CL profiles in the samples with and without catalase.…”

Section: Resultsmentioning

confidence: 54%

“…13) No CL was detected after the addition of NaClO when catalase was incubated with luminol and DTPA in the absence of H 2 O 2 (data not shown). The H 2 O 2 -dependent CL emission is indeed controlled by two-electron oxidation of the luminol/NaClO system 13,[18][19][20][21][22] and is not influenced by other mechanisms such as those involving catalase and other peroxidases. 19,23) Because the myeloperoxidase (MPO)-mediated generation of 1 O 2 was suggested in the MPO/H 2 O 2 system through the generation of HClO by MPO, 24,25) the generation of 1 O 2 was evaluated in our system, using CLA in place of luminol.…”

Section: Resultsmentioning

confidence: 93%

“…20,21) Previous investigations have shown that the small luminescence signal generated in this reaction is linear when the concentration of H 2 O 2 is in the nM to mM range. 13,22) In our study, the CL assay has been used as an indispensable analytical method for determining H 2 O 2 and evaluating the antioxidative activity of flavonoids.…”

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method

Doronicheva

Yasui

2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The antioxidative activity of flavonoids depends upon a combination of many factors, such as the concentration and chemical structure of the flavonoids and the arrangement of functional groups in their structure. In the present study, to evaluate the antioxidative effect of several types of flavonoids on catalase activity at a physiological H 2 O 2 concentration, a chemiluminescent (CL) method was used. The H 2 O 2 /luminol-dependent CL intensity in a system containing 3.7 nM catalase and low concentrations (10-100 nM) of green tea flavanols (epigallocatechin gallate; EGCG and epicatechin gallate; EG) was enhanced in comparison with that of a system without catalase, suggesting that EGCG and EG partially suppressed catalase activity. On the other hand, flavone and flavonols such as rutin (a 3-glycosidic flavone), quercitrin (a 3-glycosidic flavonol), myricetin, and kaempferol (flavonols), respectively, lowered the CL intensity to a greater extent at low concentrations (Ͻ0.1 m mM) when catalase was present than when catalase was absent, indicating that these flavonoids activate catalase. In addition, isoflavone and flavanone such as daidzein and naringenin, respectively, exhibited weak antioxidative activities against H 2 O 2 without any effect on the catalase activity over a wide range of flavonoid concentrations (0.04-0.4 m mM). From these results, it was for the first time suggested that the binding of flavonoids to the heme moiety or a protein region of catalase contributes to the enhancement of catalase activity.

show abstract

Section: Resultsmentioning

confidence: 54%

Section: Resultsmentioning

confidence: 93%

See 1 more Smart Citation

Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method

Doronicheva

Yasui

2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Using the procedure of Arnhold et al [42] , luminol (Sigma), isotonic phosphate buffer, H 2 O 2 and thymol were preincubated in a fi nal volume of 800 l for 30 s in a cuvette placed in the Luminometer 1250, and then 200 l of NaOCl were injected.The fi nal concentrations were H 2 O 2 8 ! 10 -6 mol/l, luminol 2 !…”

Section: Luminol-hypochlorous Acid/h 2 O 2 Systemmentioning

confidence: 99%

Antioxidant Potential of Thymol Determined by Chemiluminescence Inhibition in Human Neutrophils and Cell-Free Systems

et al. 2006

View full text Add to dashboard Cite

Thyme essential oil and thymol have antimicrobial, antifungal and antioxidant activities. Their antioxidant activity has been studied almost exclusively by means of chemical testing in order to be able to use it for food preservation purposes. The aim of this luminol amplified chemiluminescence (LACL) study was to investigate whether thymol can interfere with the production of reactive oxygen species, nitric oxide and the nitric oxide-derived peroxynitrite released by human neutrophils after activation by fMLP and PMA with and without the addition of the L-arginine (L-Arg) nitric oxide donor to the medium. The lowest thymol concentration that was still active in reducing LACL was 2.73 µg/ml, and there was a progressive linear inhibition of LACL from this concentration to 21.87 µg/ml, the highest thymol concentration investigated. This was also observed in the case of both fMLP and PMA stimulation with or without L-Arg. In cell-free systems using H₂O₂/HOCl^– and SIN-1 as radical producers, a significant scavenging activity of thymol was present already at 0.08 and 0.68 µg/ml respectively, and these are very low concentrations. These findings can be related to the phenolic structure of thymol, because phenolic compounds have redox properties and play an important role in adsorbing and neutralizing free radicals and peroxynitrite, and in decomposing peroxides. Our findings in human neutrophils are pharmacologically relevant as they imply that thymol is a potential antioxidant and anti-inflammatory agent in human cells.

show abstract

“…Afterwards, NaOCl (2.5 x 10 -7 mol/l, final concentration) was injected and chemiluminescence response monitored, inder experimental conditions described previously by Arnhold et al [16]. Additionally, butanol and staurosporine were added directly to the supernatant of the cytochalasin B/fMet-Leu-Phe stimulated cells, and chemiluminescence was measured.…”

Section: Measurement Of Oxidative Activitymentioning

confidence: 99%

Involvement of Phosphatidic Acid in both Degranulation and Oxidative Activity in fMet-Leu-Phe Stimulated Polymorphonuclear Leukocytes

Vocks¹,

Petković²,

Arnhold³

2003

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aim: Polymorphonuclear leukocytes (neutrophils) release a variety of toxic agents – proteins and reactive oxygen species (ROS) – that are used to inactivate foreign microorganisms in the non-specific immune response. This study was undertaken to compare intracellular signalling pathways that lead to the ROS production as well as degranulation of azurophilic granules of human fMet-Leu-Phe/cytochalasin B stimulated neutrophils. Methods: Luminol-amplified chemiluminescence was used for monitoring the oxidative activity of human neutrophils in the presence of various inhibitors. The elastase activity was assessed in the neutrophil supernatant as a marker for degranulation of azurophilic granules. Results: Tested inhibitors of enzymes of signalling cascades showed the same effect on the ROS production and on the activity of elastase released from neutrophils. The only difference was obtained with staurosporine: it inhibited the chemiluminescence response, but increased the elastase release. Conclusion: Early signalling pathways leading to the ROS production and the degranulation are ubiquitous in human neutrophils. They are branching most probably at the point of the phosphatidic acid production by phospholipase D. A protein kinase activated by this lipid second messenger might play a central regulatory role in human neutrophils.

show abstract

Mechanisms of inhibition of chemiluminescence in the oxidation of luminol by sodium hypochlorite

Cited by 50 publications

References 26 publications

Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method

Chemical Structure-Dependent Differential Effects of Flavonoids on the Catalase Activity as Evaluated by a Chemiluminescent Method

Antioxidant Potential of Thymol Determined by Chemiluminescence Inhibition in Human Neutrophils and Cell-Free Systems

Involvement of Phosphatidic Acid in both Degranulation and Oxidative Activity in fMet-Leu-Phe Stimulated Polymorphonuclear Leukocytes

Contact Info

Product

Resources

About