Mechanisms of macrophage activation by Corynebacterium parvum

Christie, G. H.; Bomford, R.

doi:10.1016/s0008-8749(75)80014-1

Cited by 62 publications

(19 citation statements)

References 28 publications

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“…The uptake of this vaccine by these cells may be important in the initiation of the response. At present, it is unclear whether T cells are also required for the anti-tumour action of C. parvum (Woodruff, Dunbar and Ghaffar 1973;Christie and Bomford, 1975;Sadler and Castro, 1976b).…”

Section: Discussionmentioning

confidence: 99%

Radiolabelling of Corynebacterium parvum and its distribution in mice

Sadler¹,

Cramp²,

Castro³

1977

Br J Cancer

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Corynebacterium parvum was labelled by growing live bacteria in the presence of [3H]thymidine. The bacteria were killed by formalin, washed thoroughly and resuspended at a concentration of 7 mg dry weight/ml. An activity of 1-6 X 10(5) ct/min/0-1 ml was obtained. The biological properties (inhibition of tumour growth and hepatosplenomegaly) of the labelled C. parvum were compared with those of commercially available vaccine, and were found to be similar. Labelled C. parvum was injected i.v., i.p., or s.c. into normal C57BL mice and the localization of activity determined at 4 h and 1,3,7 and 14 days after injection. After i.v. or i.p. injection, highest counts were recorded in the liver. Moderate activity was found in the spleen, lungs and small gut. After s.c. injection, the majority of radioactive label was detected at the site of injection and little found in other tissues. The distribution of injected C. parvum was also studied in mice bearing Lewis tumour, and was found to be similar to that in normal mice. Moderate amounts of labelled C. parvum were recovered from tumour. There appeared to be no relationship between the antitumour effect of C. parvum given by a particular route of injection and the concentration of C. parvum recovered from the tumour. Images Fig. 7 Fig. 8

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Section: Discussionmentioning

confidence: 99%

Radiolabelling of Corynebacterium parvum and its distribution in mice

Sadler¹,

Cramp²,

Castro³

1977

Br J Cancer

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“…There is considerable evidence that macrophages are involved in the antitumour action of C. parvum (Ghaffar et al, 1975;Christie and Bomford, 1975). Therefore, we postulate that the mechanism of inhibition of metastases after i.v.…”

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confidence: 88%

“…parvum had no antimetastatic activity in mice that had received ALS. Recent work by Christie and Bomford (1975) has shown that peritoneal macrophages from untreated mice cannot be activated in vitro by C. parvum alone but only when C. parvum is added with 0-sensitive immune spleen cells. This suggests that the action of C. parvum on metastases is dependent upon a specific population of long-lived circulating T cells.…”

mentioning

confidence: 99%

Abrogation of the anti-metastatic activity of C. parvum by antilymphocyte serum

Sadler¹,

Castro²

1976

Br J Cancer

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“…parvum significantly decreased metastasis in mice given Si, CA or TB. This result was unexpected, as there is considerable evidence for the involvement of macrophages in the antitumour action of C. parvum (Ghaffar, Cullen and Woodruff, 1975;Christie and Bomford, 1975). However, measurement of phagocytic index showed C. parvum was able to overcome, to a limited extent, inhibition of macrophage activity produced by Si or CA, probably by increasing macrophage production (Wolmark and Fisher, 1974;Warr and Sljivic, 1974).…”

Section: Controlsmentioning

confidence: 99%

Immunological mechanisms in metastatic spread and the antimetastatic effects of C. parvum

Jones¹,

Castro²

1977

Br J Cancer

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Summary. The effects of the host's immune response on metastatic spread was investigated by observing the numbers of pulmonary metastases that developed from an s.c. implant of the Lewis lung carcinoma in C57BL mice in which different cell populations had been suppressed. Macrophage function was impaired by treatment with silica (Si), cortisone acetate (CA), or trypan blue (TB). T-cell function was depressed by adult thymectomy and sublethal irradiation, or by treatment with antilymphocyte serum (ALS).Metastasis was significantly increased and phagocytic activity decreased by Si and CA, but were unaffected by TB. Thymectomy and irradiation had no effect on metastases, whereas ALS when given before, but not after tumour growth, reduced their number.The antimetastatic action of the immunopotentiating agent C. parvum was investigated in these immunologically impaired mice. It was unaffected by Si, CA or TB. However, the inhibiting effect of these agents on phagocytic activity was overcome by treatment with C. parvum. Its antimetastatic action was unaffected in mice which had been thymectomized and irradiated, but could be abrogated by ALS. However, ALS was only able to prevent this activity if given before tumour growth; it was ineffective if given after tumour growth.This study showed that metastatic spread was inversely related to phagocytic activity. The antimetastatic effect of C. parvum appears to be mediated through macrophages in concert with a subpopulation of T lymphocytes, which were considered to be necessary in the sensitization arm of the response as opposed to the effector arm of this response.

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Mechanisms of macrophage activation by Corynebacterium parvum

Cited by 62 publications

References 28 publications

Radiolabelling of Corynebacterium parvum and its distribution in mice

Radiolabelling of Corynebacterium parvum and its distribution in mice

Abrogation of the anti-metastatic activity of C. parvum by antilymphocyte serum

Immunological mechanisms in metastatic spread and the antimetastatic effects of C. parvum

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