2019
DOI: 10.1101/590869
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Mechanisms of motor-independent membrane remodeling driven by dynamic microtubules

Abstract: Microtubule-dependent organization of membrane organelles, such as the endoplasmic reticulum, occurs through motor-based pulling and by coupling polymer dynamics to membrane remodeling. Membrane binding to dynamic microtubule ends involves transient interactions, but how such interactions can lead to membrane deformation is unclear.Here, we reconstitute membrane tubulation in a minimal system with giant unilamellar vesicles, dynamic microtubules, End-Binding (EB) proteins and a membrane-targeted polypeptide th… Show more

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Cited by 1 publication
(8 citation statements)
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References 95 publications
(78 reference statements)
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“…We posit that the motion of a substrate-bound multivalent cargo originates from the ability of its interaction sites (henceforth referred to as legs) to repeatedly bind/unbind at different positions on the substrate (figure 1). This implies that this process should be able to explain both the diffusive motion of multivalent ligand molecules interacting with receptor-functionalized surfaces [1] and the processive motion of multivalent cellular components interacting with cytoskeletal polymers [5,7,27,28,31]. Differences in cargo transport would then reflect differences in the spatial distribution of leg-substrate interaction rates.…”
Section: The Cargo Binding Modelmentioning
confidence: 99%
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“…We posit that the motion of a substrate-bound multivalent cargo originates from the ability of its interaction sites (henceforth referred to as legs) to repeatedly bind/unbind at different positions on the substrate (figure 1). This implies that this process should be able to explain both the diffusive motion of multivalent ligand molecules interacting with receptor-functionalized surfaces [1] and the processive motion of multivalent cellular components interacting with cytoskeletal polymers [5,7,27,28,31]. Differences in cargo transport would then reflect differences in the spatial distribution of leg-substrate interaction rates.…”
Section: The Cargo Binding Modelmentioning
confidence: 99%
“…Previous studies have shown that beads coated in EB binding domains can track the growing ends of microtubules in the presence of EBs [5,27], but a model that can describe these dynamics has not yet been developed. In order to test whether the model presented in this work can reproduce the dynamics of multivalent cargo in biologically relevant conditions, we first converted the parameters describing EB-microtubule interactions obtained in previous studies [23,27,[36][37][38][39][40][41] into binding/unbinding rate distributions required as inputs for our stochastic cargo binding simulations (see electronic supplementary material, §7 and table S1). Here, we have subsumed the EB layer into an effective distribution of binding/unbinding rates for the multivalent cargo.…”
Section: Using Experimentally Derived Input Parametersmentioning
confidence: 99%
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