Mechanisms of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes.

Verhoven, Bret; Schlegel, Robert; Williamson, Patrick

doi:10.1084/jem.182.5.1597

Cited by 618 publications

(475 citation statements)

References 31 publications

(33 reference statements)

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“…PC roles have been well established in differentiation and cell proliferation [43]. CCs undergo proliferation before the LH surge due to the action of growth factors secreted by the oocyte to regulate the function of the CCs [17].…”

Section: Discussionmentioning

confidence: 99%

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Montani

Cordeiro

Regiani

et al. 2012

J Assist Reprod Genet

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Purpose This research proposed to study the changes in lipid composition in cumulus cells (CCs) from women who achieved pregnancy compared with women who did not, after in vitro fertilization treatment. This approach has the potential to provide novel information on the lipid metabolism of the CCs and as an additional method to predict pregnancy. Method Fifty-four samples from couples with tubal and male factor infertility and where the female partner was age 35 or younger were divided in two groups according to their level of hCG 14 days after embryo transfer as follows: (1) 23 samples in pregnant group and (2) 31 samples in non-pregnant group. Lipid extraction was performed by the Bligh-Dyer protocol, and lipid profiles were obtained by MALDI-TOF MS. Mass spectra data were processed with MassLynx, and statistical analysis was performed using MarkerLynx extended statistic. OPLS-DA model was built. Results S-plot Analysis revealed three ions as potential markers in the pregnant group, and five ions in the nonpregnant group. These ions were identified in the human metabolome database (HMDB) as phosphatidylcholine in the pregnant group and as phosphatidylethanolamine, phosphatidylserine and phosphatidylinositol species in the non-pregnant group. These lipids might be involved in cell proliferation and differentiation, apoptosis and GAP junction regulation. Conclusion We conclude that MALDI-TOF MS can be used as an informative and fast analytical strategy to obtain and study the lipid profile of cumulus cells and can potentially be used as a supporting tool to predict pregnancy based on the metabolic state of the CCs.

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Section: Discussionmentioning

confidence: 99%

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Montani

Cordeiro

Regiani

et al. 2012

J Assist Reprod Genet

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“…Thus, annexin V-positive, PI-negative objects can be accepted as apoptotic cells only if those objects are shown to be approximately cell-size. As phospholipid asymmetry is maintained by an energy-dependent enzyme (Verhoven et al, 1995), it is likely that it would be lost in any type of subcellular fragment. If these fragments do not contain DNA or have an intact membrane, they will not stain with PI and might be recorded as apoptotic cells.…”

Section: Discussionmentioning

confidence: 99%

Apoptosis assays with lymphoma cell lines: problems and pitfalls

Mattes

2007

Br J Cancer

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Much attention has been focused on the manner in which tumour cells die after treatment with cytotoxic agents. The basic question is whether cells die via apoptosis or via direct damage from the toxic agent. Various assays have been used to make this distinction. However, we show herein that some of the widely used assays for apoptosis do not in fact distinguish between apoptosis and other forms of cell death. More specifically: (1) A sub-G 1 DNA content, identified by propidium iodide staining, does not distinguish between apoptotic and necrotic cells; (2) loss of mitochondrial membrane potential does not distinguish between apoptotic and necrotic cells, unless combined with an assay for an intact cell membrane; (3) subcellular fragments that arise from dead cells or from apoptotic bodies can interfere with some assays for apoptosis such as annexin V staining, as they may be close to the size of intact cells, making it difficult to decide where to set the size threshold; (4) irradiated cells display a large increase in nonspecific Ab binding. This may be partly due to an increase in cell size, but, regardless of the cause, it can lead to a mistaken conclusion that there is an increase in a particular antigen if appropriate control reagents are not tested; and (5) experiments utilising Ab crosslinking have neglected the role of cell aggregation, which can cause multiple problems including death from mechanical stress when cells are handled. Consideration of these factors will improve our ability to determine the mode of cell death.

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“…Externalization of membrane phosphatidyl serine (PS), one of the earliest signs of apoptosis, is detectable by Annexin V staining (Fadok et al, 1992;Martin et al, 1991;Verhoven et al, 1995). The presence of Annexin V + /PI 7 cells is indicative of preapoptotic cells, while such staining is not seen in the case of death by necrosis (Kroemer, 1995).…”

Section: Simultaneous Activation Of Her1 and Irf-1 Leads To Cell Deathmentioning

confidence: 99%

Cooperative activity between HER oncogenes and the tumor suppressor IRF-1 results in apoptosis

Kirchhoff¹,

Hauser²

1999

Oncogene

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Mechanisms of phosphatidylserine exposure, a phagocyte recognition signal, on apoptotic T lymphocytes.

Cited by 618 publications

References 31 publications

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

The follicular microenviroment as a predictor of pregnancy: MALDI-TOF MS lipid profile in cumulus cells

Apoptosis assays with lymphoma cell lines: problems and pitfalls

Cooperative activity between HER oncogenes and the tumor suppressor IRF-1 results in apoptosis

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