Mechanisms of plant cell division

Lipka, Elisabeth; Herrmann, Arvid; Müller, Sabine

doi:10.1002/wdev.186

Cited by 37 publications

(27 citation statements)

References 83 publications

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“…In such wide PPB areas defined in ktn1-2 mutants, it is hypothetically possible that markers of CDP orientation attracting cell plate deposition to the PPB site (Lipka et al, 2015) may be more diffusely localized and this would help to explain the occurence of oblique cell division planes observed in KATANIN 1 mutants such as erh3, fra2, lue1 , and ktn1-2 (e.g., Webb et al, 2002; Panteris et al, 2011). In living mitotic petiole and cotyledon epidermal cells of ktn1-2 we never observed multipolar spindles as those described before by tubulin immunofluorescence in fixed root whole mounts of fra2 and lue1 (Panteris et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…This observation suggests that KATANIN 1 activity is somehow required for spindle positional control, possibly by controlling attachment of the spindle to the cell cortex (Granger and Cyr, 2001; Kojo et al, 2013, 2014; Lipka et al, 2015). Similar motions were also exhibited by the nascent phragmoplast at the earliest stages of its formation and before its margin could become tethered to the parent wall.…”

Section: Discussionmentioning

confidence: 99%

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Katanin Effects on Dynamics of Cortical Microtubules and Mitotic Arrays in Arabidopsis thaliana Revealed by Advanced Live-Cell Imaging

et al. 2017

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Katanin is the only microtubule severing protein identified in plants so far. Previous studies have documented its role in regulating cortical microtubule organization during cell growth and morphogenesis. Although, some cell division defects are reported in KATANIN mutants, it is not clear whether or how katanin activity may affect microtubule dynamics in interphase cells, as well as the progression of mitosis and cytokinesis and the orientation of cell division plane (CDP). For this reason, we characterized microtubule organization and dynamics in growing and dividing cotyledon cells of Arabidopsis ktn1-2 mutant devoid of KATANIN 1 activity. In interphase epidermal cells of ktn1-2 cortical microtubules exhibited aberrant and largely isotropic organization, reduced bundling and showed excessive branched microtubule formation. End-wise microtubule dynamics were not much affected, although a significantly slower rate of microtubule growth was measured in the ktn1-2 mutant where microtubule severing was completely abolished. KATANIN 1 depletion also brought about significant changes in preprophase microtubule band (PPB) organization and dynamics. In this case, many PPBs exhibited unisided organization and splayed appearance while in most cases they were broader than those of wild type cells. By recording PPB maturation, it was observed that PPBs in the mutant narrowed at a much slower pace compared to those in Col-0. The form of the mitotic spindle and the phragmoplast was not much affected in ktn1-2, however, the dynamics of both processes showed significant differences compared to wild type. In general, both mitosis and cytokinesis were considerably delayed in the mutant. Additionally, the mitotic spindle and the phragmoplast exhibited extensive rotational motions with the equatorial plane of the spindle being essentially uncoupled from the division plane set by the PPB. However, at the onset of its formation the phragmoplast undergoes rotational motion rectifying the expansion of the cell plate to match the original cell division plane. Conclusively, KATANIN 1 contributes to microtubule dynamics during interphase, regulates PPB formation and maturation and is involved in the positioning of the mitotic spindle and the phragmoplast.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Katanin Effects on Dynamics of Cortical Microtubules and Mitotic Arrays in Arabidopsis thaliana Revealed by Advanced Live-Cell Imaging

et al. 2017

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“…During cytokinesis, the physical partitioning of all cellular content, plant cells separate by an inward-to-outward directed division mode of centrifugal cell plate assembly, as opposed to a centripetal furrowing predominant in metazoan cytokinesis [1][2][3]. The plant cytokinetic apparatus, called phragmoplast, aids in cell plate biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3

et al. 2018

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Kinesins are versatile nano-machines that utilize variable non-motor domains to tune specific motor microtubule encounters. During plant cytokinesis, the kinesin-12 orthologs, PHRAGMOPLAST ORIENTING KINESIN (POK)1 and POK2, are essential for rapid centrifugal expansion of the cytokinetic apparatus, the phragmoplast, toward a pre-selected cell plate fusion site at the cell cortex. Here, we report on the spatio-temporal localization pattern of POK2, mediated by distinct protein domains. Functional dissection of POK2 domains revealed the association of POK2 with the site of the future cell division plane and with the phragmoplast during cytokinesis. Accumulation of POK2 at the phragmoplast midzone depends on its functional POK2 motor domain and is fine-tuned by its carboxy-terminal region that also directs POK2 to the division site. Furthermore, POK2 likely stabilizes the phragmoplast midzone via interaction with the conserved microtubule-associated protein MAP65-3/PLEIADE, a well-established microtubule cross-linker. Collectively, our results suggest that dual localized POK2 plays multiple roles during plant cell division.

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“…Knockouts of any protein from POK1, TAN and RANGAP1 results in cytokinesis defects. 1,2,3 It is considered, that in late cytokinesis POK1 is associated with peripheral MTs and provides directions for cell plate expansion. 1 Another kinesin NACK1 (kinesin-7 HIN-KEL) is an upstream member of the NACK1/PQR pathway which regulates MTs turnover and promotes cell plate expansion.…”

Section: Introductionmentioning

confidence: 99%

“…1,2,3 It is considered, that in late cytokinesis POK1 is associated with peripheral MTs and provides directions for cell plate expansion. 1 Another kinesin NACK1 (kinesin-7 HIN-KEL) is an upstream member of the NACK1/PQR pathway which regulates MTs turnover and promotes cell plate expansion. 4 We recently reported a cytokinesis defect, characterized by inhibition of the late cell plate expansion and disturbed AFs dynamics, in BY-2 cells 5 which occurs after treatment with ATP-competitive inhibitor of mitotic kinase Haspin, 5-Iodotubercidin (5-ITu), at final concentration 1 mM.…”

Section: Introductionmentioning

confidence: 99%

Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors

Kozgunova

Higashiyama

Kurihara

2016

Plant Signaling & Behavior

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Cytokinesis is last but not least in cell division as it completes the formation of the two cells. The main role in cell plate orientation and expansion have been assigned to microtubules and kinesin proteins. However, recently we reported severe cytokinesis defect in BY-2 cells not accompanied by changes in microtubules dynamics. Here we also confirmed that distribution of kinesin NACK1 is not the cause of cytokinesis defect. We further explored inhibition of the cell plate expansion by ATP-competitive inhibitors. Two different inhibitors, 5-Iodotubercidin and ML-7 resulted in a very similar phenotype, which indicates that they target same protein cascade. Interestingly, in our previous study we showed that 5-Iodotubercidin treatment affects concentration of actin filaments on the cell plate, while ML-7 is inhibitor of myosin light chain kinase. Although not directly, it indicates importance of actomyosin complex in plant cytokinesis.Abbreviations: AFs, actin filaments; BY-2, Bright Yellow-2; DMSO, dimethylsulfoxide; GFP, green fluorescent protein; 5-ITu, 5-iodotubercidin; MTs, mictrotubules KEYWORDS 5-Iodotubercidin; actomyosin role in plant cytokinesis; cytokinesis defect; Haspin kinase; inhibition of the cell plate expansion; kinesin NACK1; Cytokinesis in plants is essentially a process of building a new cell wall that separates two daughter cells. It is achieved by phragmoplast-guided vesicle transport to the division plane and vesicle fusion that forms a precursor of the cell wall. Dynamic changes in the phragmoplast are essential for proper cell plate formation and expansion; however, our understanding of what triggers and controls those changes is far from complete.Phragmoplast consists from microtubules (MTs), actin filaments (AFs) and associated proteins. MTs act as a driving force for vesicle trafficking and cell plate expansion. Kinesin proteins interact with MTs, providing spatial information for cell plate guidance or affecting their stability. Kinesin POK1 (phragmoplast-orientating kinesin-12) together with TAN and RAN-GAP1 is a part of pre-prophase band and during mitosis is localized to the cortical division zone. Knockouts of any protein from POK1, TAN and RANGAP1 results in cytokinesis defects.

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Mechanisms of plant cell division

Cited by 37 publications

References 83 publications

Katanin Effects on Dynamics of Cortical Microtubules and Mitotic Arrays in Arabidopsis thaliana Revealed by Advanced Live-Cell Imaging

Katanin Effects on Dynamics of Cortical Microtubules and Mitotic Arrays in Arabidopsis thaliana Revealed by Advanced Live-Cell Imaging

Dual localized kinesin‐12 POK 2 plays multiple roles during cell division and interacts with MAP 65‐3

Cytokinesis defect in BY-2 cells caused by ATP-competitive kinase inhibitors

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