Mechanisms of Protein Folding by Type II Chaperonins

Plimpton, Rebecca L.; Valpuesta, José; Willardson, Barry M.

doi:10.2174/9781681086156118010008

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased levels of heat shock proteins may trigger a cell survival/resistance mechanism in cancer cells. Additionally, HSPs activate the angiogenic pathway that leads to vascular endothelial growth factor (VGEF) expression, vessel formation, and accelerated tumor formation in host tissues (Chatterjee et al, 2018;Plimpton et al, 2018;Solis et al, 2019). Jun N-terminal Kinases (JNKs) are small proteins, 45-55 kDa, and product of three genes which, through different splicing, create up to 10 isoforms (Alvarez et al, 1991;Hibi et al, 1993).…”

Section: Phospho Mapk Array-phosphorylated Analytes In Bp Mc7 Bccsmentioning

confidence: 99%

Thermal Bioprinting Causes Ample Alterations of Expression of LUCAT1, IL6, CCL26, and NRN1L Genes and Massive Phosphorylation of Critical Oncogenic Drug Resistance Pathways in Breast Cancer Cells

Mohl

Gutierrez

Varela-Ramı́rez

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Bioprinting technology merges engineering and biological fields and together, they possess a great translational potential, which can tremendously impact the future of regenerative medicine and drug discovery. However, the molecular effects elicited by thermal inkjet bioprinting in breast cancer cells remains elusive. Previous studies have suggested that bioprinting can be used to model tissues for drug discovery and pharmacology. We report viability, apoptosis, phosphorylation, and RNA sequence analysis of bioprinted MCF7 breast cancer cells at separate timepoints post-bioprinting. An Annexin A5-FITC apoptosis stain was used in combination with flow cytometry at 2 and 24 h post-bioprinting. Antibody arrays using a Human phospho-MAPK array kit was performed 24 h post-bioprinting. RNA sequence analysis was conducted in samples collected at 2, 7, and 24 h post-bioprinting. The post-bioprinting cell viability averages were 77 and 76% at 24 h and 48 h, with 31 and 64% apoptotic cells at 2 and 24 h after bioprinting. A total of 21 kinases were phosphorylated in the bioprinted cells and 9 were phosphorylated in the manually seeded controls. The RNA seq analysis in the bioprinted cells identified a total of 12,235 genes, of which 9.7% were significantly differentially expressed. Using a ±2-fold change as the cutoff, 266 upregulated and 206 downregulated genes were observed in the bioprinted cells, with the following 5 genes uniquely expressed NRN1L, LUCAT1, IL6, CCL26, and LOC401585. This suggests that thermal inkjet bioprinting is stimulating large scale gene alterations that could potentially be utilized for drug discovery. Moreover, bioprinting activates key pathways implicated in drug resistance, cell motility, proliferation, survival, and differentiation.

show abstract