Mechanisms of Resistance to Glyphosate in a Ryegrass (<i>Lolium Multiflorum</i>) Biotype from Chile

Michitte, P; Prado, Rafael De; Espinoza, Nelson; Ruiz-Santaella, J. P.; Gauvrit, Christian

doi:10.1614/ws-06-167.1

Cited by 108 publications

(127 citation statements)

References 15 publications

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“…Differences in the response of plants to the exposure to glyphosate can be derived from differences in spray retention or/and drop contact angle in the leaves (Chachalis et al, 2001), (Norsworthy et al, 2001;Michitte et al, 2007), composition of the leaf epicuticular wax (Michitte et al, 2004;Nandula et al, 2008;Guimarães et al, 2009;Hatterman-Valenti et al, 2011), herbicide absorption or/and translocation (Dinelli et al, 2008;Guimarães et al, 2009;Ge et al, 2010;Carvalho et al, 2012b), and herbicide degradation (RojanoDelgado et al, 2010(RojanoDelgado et al, , 2012Carvalho et al, 2012bCarvalho et al, , 2013b. Thus, any difference in the herbicide absorption, translocation, or/and metabolism can influence the plant response to glyphosate, culminating in a higher or lower susceptibility to this herbicide.…”

Section: Resultsmentioning

confidence: 99%

Plant Growth Responses of Apple and Pear Trees to Doses of Glyphosate

et al. 2016

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-Glyphosate is commonly used for intra-row weed management in perennial plantations, where unintended crop exposure to this herbicide can cause growth reduction. The objective of this research was to analyze the initial plant growth behavior of young apple and pear plants exposed to glyphosate. Glyphosate was sprayed on 2-year-old 'Gala' apple and 'Abbè Fetel' pear plants at doses from 18 to 720 g per hectare of acid equivalent (a.e.). The plant height of neither species was not significantly reduced (less than 1%) by any glyphosate dose at 240 days after spraying, whereas the stem diameter and the dry mass of stem and leaves were reduced by 720 g a.e. ha -1 . The glyphosate dose required to reduce the aboveground dry mass by 50% was 162 and 148 g a.e. ha -1 for apple and pear, respectively. Aboveground dry mass was reduced 2% and 6% for apple and pear plants, respectively, at 720 g a.e. ha -1 . Hormesis was not observed in either species at doses down to 18 g a.e. ha -1 . Both species showed low susceptibility to glyphosate; however apple was less susceptible than pear. Keywords: N-phosphonomethyl-glycine, Malus domestica, Pyrus communis, spray drift. RESUMO -Glyphosate é comumente usado para manejo de plantas daninhas na linha de plantio de culturas perenes, onde a exposição não intencional da cultura a esse herbicida pode causar redução do crescimento. O objetivo desta pesquisa foi analisar o comportamento do crescimento inicial de

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Section: Resultsmentioning

confidence: 99%

Plant Growth Responses of Apple and Pear Trees to Doses of Glyphosate

et al. 2016

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“…It is very unlikely that these rare single genes were present in our originator susceptible population VLR1, given the very small number of plants that we had exposed to glyphosate selection (Yu et al, 2007). Aside from these two single-gene-endowed glyphosate resistance mechanRecurrent selection at sublethal glyphosate doses isms, reduced foliar retention of glyphosate and differential uptake have also been reported in Lolium (Michitte et al, 2007). In our low-dose glyphosate-selected population, the mechanistic basis of resistance remains to be identified.…”

Section: Discussionmentioning

confidence: 96%

Evolution of glyphosate resistance in a Lolium rigidum population by glyphosate selection at sublethal doses

2009

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The majority of the documented cases of field-evolved herbicide-resistant weed biotypes established that single major genes confer glyphosate resistance. However, the contribution of minor genes endowing substantial plant survival at sublethal herbicide doses may be a potential complementary path to herbicide resistance evolution in weed populations under selection. Here, we subjected a number of susceptible individuals of Lolium rigidum to recurrent glyphosate selection to test the potential for sublethal glyphosate doses to additively select for glyphosate resistance. After 3-4 cycles of glyphosate selection in two distinct environments, the progenies of the initially susceptible population were shifted toward glyphosate resistance. The results indicate progressive enrichment of minor gene trait(s) contributing toward plant survival in the glyphosate-selected progenies. After three generations of selection, the estimated LD 50 values were doubled compared with the original population and up to 33% plant survival was obtained in the glyphosate-selected progeny at the recommended glyphosate label rate. This level of resistance probably was the maximum shift achievable with sublethal glyphosate dose selection in this small population. Cross-pollination was a crucial factor enabling the rapid rate of accumulation of minor glyphosate resistance gene trait(s) that are likely to be present at a relatively high frequency in a small susceptible population. The mechanistic basis of the moderate glyphosate resistance level selected by sublethal glyphosate doses remains unknown and warrants future research. Studying the main factors influencing the evolution of resistant weed populations is crucial for understanding, predicting and managing herbicide resistance.

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“…In fact, resistant biotypes of this species show reduced glyphosate absorption through the abaxial leaf surface and higher concentration of 14 C1180-glyphosate at the tip of the leaves (Michitte et al, 2007). Reduction of absorption A modified phosphate-carrier protein theory is proposed as ... and translocation of glyphosate were also identified in L. multiflorum from Mississippi (Nandula et al, 2008).…”

Section: Reduction Of Absorption or Translocationmentioning

confidence: 93%

A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds

Roso

Vidal

2010

Planta daninha

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-Glyphosate is an herbicide that inhibits the enzyme 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPs) (EC 2.5.1.19). EPSPs is the sixth enzyme of the shikimate pathway, by which plants synthesize the aromatic amino acids phenylalanine, tyrosine, and tryptophan and many compounds used in secondary metabolism pathways. About fifteen years ago it was hypothesized that it was unlikely weeds would evolve resistance to this herbicide because of the limited degree of glyphosate metabolism observed in plants, the low resistance level attained to EPSPs gene overexpression, and because of the lower fitness in plants with an altered EPSPs enzyme. However, today 20 weed species have been described with glyphosate resistant biotypes that are found in all five continents of the world and exploit several different resistant mechanisms. The survival and adaptation of these glyphosate resistant weeds are related toresistance mechanisms that occur in plants selected through the intense selection pressure from repeated and exclusive use of glyphosate as the only control measure. In this paper the physiological, biochemical, and genetic basis of glyphosate resistance mechanisms in weed species are reviewed and a novel and innovative theory that integrates all the mechanisms of non-target site glyphosate resistance in plants is presented.Keywords: membrane carrier proteins, herbicide, weed resistance.RESUMO -Glyphosate é uma glicina fosfonada e inibe a enzima 5-enolpiruvil-shikimato-3-fosfato sintase (EPSPS) (EC 2.5.1.19). EPSPS é a sexta enzima da rota do shikimato, na qual são sintetizados os compostos do metabolismo secundário e os aminoácidos aromáticos fenilalanina, tirosina e triptofano. Alguns autores hipotetizaram que seria improvável a evolução de plantas daninhas resistentes a este herbicida. As justificativas estariam relacionadas à limitada metabolização de glyphosate nas plantas, ao baixo nível de resistência obtido com a superexpressão do gene EPSPS e à alta penalidade de adaptação oriunda de mutações no gene EPSPS. Mas, atualmente estão descritas 18 espécies com biótipos resistentes distribuídas em 17 países. A sobrevivência e adaptação estão relacionadas aos mecanismos de resistência que ocorrem nas plantas daninhas selecionadas em função da intensa pressão de seleção do herbicida. Esta revisão objetiva apresentar e discutir evidências fisiológicas, bioquímicas e genéticas que fundamentam os principais mecanismos envolvidos na resistência ao herbicida glyphosate em plantas daninhas.Palavras-chave: herbicida, proteínas carregadoras de membrana, planta daninha.

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Mechanisms of Resistance to Glyphosate in a Ryegrass (Lolium Multiflorum) Biotype from Chile

Cited by 108 publications

References 15 publications

Plant Growth Responses of Apple and Pear Trees to Doses of Glyphosate

Plant Growth Responses of Apple and Pear Trees to Doses of Glyphosate

Evolution of glyphosate resistance in a Lolium rigidum population by glyphosate selection at sublethal doses

A Modified phosphate-carrier protein theory is proposed as a non-target site mechanism For glyphosate resistance in weeds

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