Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum

Jaime-Pérez, Noelia; Kaftan, David; Bína, David; Bokhari, Syed Nadeem Hussain; Shreedhar, Sowmya; Küpper, Hendrik

doi:10.1016/j.bbabio.2019.06.004

Cited by 24 publications

(15 citation statements)

References 30 publications

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“…Exposure to toxic Cu levels induces the formation of reactive oxygen species (ROS) inside plant cells, which leads to an imbalance in red-ox homeostasis [ 41 , 42 ]. The accumulation of ROS generates increasing oxidation of tissue components that can switch on a multitude of damaging effects, including injury of membranes, nucleic acids, proteins, and carbohydrates [ 43 ], finally causing the death of the organism [ 6 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Main Molecular Pathways Associated with Copper Tolerance Response in Imperata cylindrica by de novo Transcriptome Assembly

Vidal

Larama

Riveros

et al. 2021

Plants

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The metallophyte Imperata cylindrica inhabits copper (Cu) polluted soils in large areas from Central Chile. Here, we subjected clonal vegetative plantlets to 300 mg Cu kg−1 of substrate for 21 days to identify the main molecular pathways involved in the response to Cu stress. Transcriptomic analyses were performed for shoots and roots, with and without Cu supply. RNA-Seq and de novo transcriptome assembly were performed to identify the gene response associated with molecular mechanisms of Cu tolerance in I. cylindrica. De novo transcriptome revealed a total of 200,521 transcripts (1777 bp) comprising ~91% complete ultra-conserved genes in the eukaryote and Plantae database. The differentially expressed genes (DEGs) in roots were 7386, with 3558 of them being up-regulated and the other 3828 down-regulated. The transcriptome response in shoots was significantly less, showing only 13 up-regulated and 23 down-regulated genes. Interestingly, DEGs mainly related with actin and cytoskeleton formation, and to a minor degree, some DEGs associated with metal transporters and superoxide dismutase activity in root tissues were found. These transcriptomic results suggest that cytoskeleton could be acting as a mechanism of Cu-binding in the root, resulting in a high Cu tolerance response in this metallophyte, which deserve to be analyzed ultra-structurally. Our study contributes to reinforcing the potential of I. cylindrica as a candidate plant species to be used as a phytoremediation agent in Cu-contaminated environments.

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Section: Discussionmentioning

confidence: 99%

Main Molecular Pathways Associated with Copper Tolerance Response in Imperata cylindrica by de novo Transcriptome Assembly

Vidal

Larama

Riveros

et al. 2021

Plants

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“…The qualitative result that Cupeaks can be seen in the acetone extract is shown in Figure 8b; the full analysis is the topic of a separate study. 14 A later repeat of the analysis on a metal-free column confirmed the Cu binding to the pigment but showed an improved signal/ background ratio because no Cu leached from the column housing (Figure S1).…”

Section: Application Examplesmentioning

confidence: 92%

“…Harvested cells were kept at −80 °C. Further details are described in the full study of Cu-stress in this organism by Jaime-Peŕez et al 14 Protein Isolation. Protein Extracts for Metalloproteomics.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…The isolation of complexes was performed using the method described by Picorel et al 15 with some modifications. 14 Protein Measuring Conditions. Generally, for optimizing the molecular weight (MW) range and resolution in SEC, combinations of 2−3 Superdex Increase or Superose Increase 10 × 300 mm columns (all GE Healthcare, USA) were used in series.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…Harvested cells were kept at −80 °C. Further details are described in the full study of Cu-stress in this organism by Jaime-Pérez et al…”

Section: Experimental Sectionmentioning

confidence: 99%

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Ultratrace Metal Speciation Analysis by Coupling of Sector-Field ICP-MS to High-Resolution Size Exclusion and Reversed-Phase Liquid Chromatography

et al. 2019

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Techniques for metal speciation analysis with subnanomolar (ppt) detection limits in complex matrices, with simultaneous quantification of matrix elements, have become a necessity for investigating targets of trace metal binding to macromolecules and pigments at environmentally relevant concentrations. In this work we optimized the analysis of such metal binding in a custom-built HPLC-ICP-MS system. Key elements of the optimization were the choice of components for the metal-free HPLC-DAD system and sector-field ICP-MS detection (ICF-sfMS) with desolvating injection and optimization of sample handling. Protein analysis was done using ammonium bicarbonate buffer and size exclusion chromatography (SEC-ICP-sfMS), with possible addition of anion exchange chromatography. Detection of metal exchange in pigments (chlorophylls and bacteriochlorophylls) was based on reversed-phase chromatography with a methanol-acetone gradient and coupling to the ICP-sfMS via a dedicated organic matrix interface (RPC-ICP-sfMS). The resulting HPLC-DAD-ICP-sfMS system has detection limits in the picomolar range in protein buffer, limited by the maximal achievable purity of buffers/solvents and not by system sensitivity. Tests for method optimization showed that sonication, meant to increase protein solubilization, leads to artifacts of metal loss from metalloproteins. Examples for Cd binding to soybean proteins and chlorophyll, Cr binding to Arabidopsis thaliana proteins, La binding to Desmodesmus quadricauda proteins, and Cu binding to Rhodospirillum rubrum proteins and pigments are shown. These application examples demonstrate that the system is sensitive enough to detect binding of metals to proteins and pigments at background concentration levels of typical nutrient solutions made from analytical grade chemicals, equivalent to ultratrace metal concentrations in nonpolluted environments.

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Environmental and Health Effects of Heavy Metals and Their Treatment Methods

Haroon

Rizwan

Ahmed

2022

Emerging Contaminants and Associated Treatment Technologies

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Mechanisms of sublethal copper toxicity damage to the photosynthetic apparatus of Rhodospirillum rubrum

Cited by 24 publications

References 30 publications

Main Molecular Pathways Associated with Copper Tolerance Response in Imperata cylindrica by de novo Transcriptome Assembly

Main Molecular Pathways Associated with Copper Tolerance Response in Imperata cylindrica by de novo Transcriptome Assembly

Ultratrace Metal Speciation Analysis by Coupling of Sector-Field ICP-MS to High-Resolution Size Exclusion and Reversed-Phase Liquid Chromatography

Environmental and Health Effects of Heavy Metals and Their Treatment Methods

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