Mechanisms of the beneficial effect of sevoflurane in liver ischemia/reperfusion injury

Abstract

PURPOSE:To evaluate the underlying mechanisms by which sevoflurane protects the liver against ischemia/reperfusion injury evaluate the mechanism by which sevoflurane exerts this protective effect. METHODS:Twenty-six rats were subjected to partial ischemia/reperfusion injury for 1h: one group received no treatment, one group received sevoflurane, and sham group of animals received laparotomy only. Four hours after reperfusion, levels of alanine and aspartate aminotransferases, tumor necrosis factor-α, and interleukins 6 and 10 were measured. Analyses of mitochondrial oxidation and phosphorylation, malondialdehyde content, histology, and pulmonary vascular permeability were performed. RESULTS:Serum levels of alanine and aspartate aminotransferases were significantly lower in the sevoflurane group compared to untreated controls (p<0.05). The sevoflurane group also showed preservation of liver mitochondrial function compared to untreated controls (p<0.05). Sevoflurane administration did not alter increases in serum levels of tumor necrosis factor-α, and interleukins 6 and 10. Sevoflurane treatment significantly reduced the coagulative necrosis induced by ischemia/reperfusion (p<0.05). Pulmonary vascular permeability was preserved in the sevoflurane group compared to untreated controls. CONCLUSION:Sevoflurane administration protects the liver against ischemia/reperfusion injury, via preservation of mitochondrial function, and also preserves lung vascular permeability.

Sevoflurane Preconditioning plus Postconditioning Decreases Inflammatory Response with Hemodynamic Recovery in Experimental Liver Ischemia Reperfusion

“…Animals showed decreased AST and ALT levels with liver preconditioning with sevoflurane for 15 min. Previously, in this laboratory, Cavalcante et al [18] showed decreased transaminase levels with continuous sevoflurane anesthesia for about 4 h of the whole experiment. In a randomized study in liver surgery, Beck-Schimmer et al [7] showed decreased AST and ALT levels in patients preconditioned with sevoflurane before liver ischemia.…”

“…They also demonstrated a dose-dependent decrease in TNF- α , IL-6, and IL-10 levels. However, Cavalcante et al [18] failed to demonstrate a decrease in the cytokine levels after conditioning with sevoflurane for 4 h during the experimental liver IR. In the present study, only sevoflurane preconditioning associated to postconditioning induced lower plasma IL-6 levels, without significant changes in IL-10 and TNF- α levels.…”

Isobaric tags for relative and absolute quantitation‑based proteomics reveals potential novel biomarkers for the early diagnosis of acute myocardial infarction within 3�h

“…Sun and Yang found that metformin improved the myocardial function of mice with heart failure following MI according to regulation of the expression of SIRT3 and PGC-1α in the mitochondrial inner membrane (58). However, mitochondrial dysfunction is not unique to MI; energy metabolism barriers can also occur in acute cerebral ischemia (59), renal ischemia (60), liver ischemia (61) and other ischemic diseases. This provides a novel direction in order to understand the process of MI, and further investigations are required.…”

Effect of Hepatic Artery Spasm on a Rat Model of Hepatic Ischemia‐Reperfusion Injury

“…In addition, the time required for the contrast agent to reach a peak in the liver parenchyma was shorter; the AUC was larger; and the liver parenchyma was fully developed at the peak time without an obvious dark space, illustrating that the liver microcirculation perfusion had sufficiently recovered. These results may have been due to treatment with PHT, which expanded the hepatic artery and the small branches of the artery in the liver, relieved the arterial spasm, increased liver perfusion, and improved the hepatic microcirculation …”