Mechanisms of TiO 2 NPs-induced phoxim metabolism in silkworm ( Bombyx mori ) fat body

Hu, Ji; Li, F.C.; Xu, Kai; Ni, Min; Wang, B.B.; Tian, Jianmei; Li, Y.Y.; Shen, Weide; Li, B.

doi:10.1016/j.pestbp.2015.11.004

Cited by 24 publications

(11 citation statements)

References 35 publications

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“…15). Incidentally, all the three identified lipoproteins and thiol peroxiredoxin are antioxidant proteins having anti-apoptotic functions 35–39 . Cellular detoxification plays an important role in silk gland development 40 .…”

Section: Resultsmentioning

confidence: 99%

Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Ali

Bovilla

Mysarla

et al. 2017

Sci Rep

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Bombyx mori (B. mori) is important due to its major role in the silk production. Though DNA binding ligands often influence gene expression, no attempt has been made to exploit their use in sericulture. The telomeric heterochromatin of B. mori is enriched with 5′-TTAGG-3′ sequences. These sequences were also found to be present in several genes in the euchromatic regions. We examined three synthetic oligopyrrole carboxamides that target 5′-TTAGG-3′ sequences in controlling the gene expression in B. mori. The ligands did not show any defect or feeding difference in the larval stage, crucial for silk production. The ligands caused silencing of various isoforms of the broad-complex transcription factor and cuticle proteins which resulted in late pupal developmental defects. Furthermore, treatment with such drugs resulted in statistically enhanced cocoon weight, shell weight, and silk yield. This study shows for the first time use of oligopyrrole carboxamide drugs in controlling gene expression in B. mori and their long term use in enhancing silk production.

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Section: Resultsmentioning

confidence: 99%

Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Ali

Bovilla

Mysarla

et al. 2017

Sci Rep

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“…Following the method of Hu et al. (), fifth‐instar larvae were divided into four groups with 300 silkworms in each group: control group, NAC group, imidacloprid group, and NAC + imidacloprid group. The control group and the imidacloprid group silkworms were fed with mulberry leaves dipped in clean water, whereas the NAC group and the NAC + imidacloprid group were fed with mulberry leaves dipped in NAC solution, all silkworms were fed four times daily.…”

Section: Methodsmentioning

confidence: 99%

Preventive effects of N‐acetyl‐l‐cysteine against imidacloprid intoxication on Bombyx mori larvae

Gao

Sun

Song

et al. 2018

Arch Insect Biochem Physiol

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Imidacloprid, a widely used neonicotinoid insecticide, is toxic to silkworm (Bombyx mori). To explore whether N-acetyl-l-cysteine (NAC) has an effect on preventing silkworm (B. mori) from toxification caused by imidacloprid, we fed the fifth-instar larvae with mulberry leaves dipped in 200 mg/L NAC solution before exposing in imidacloprid, and investigated the silkworm growth, survival rate, feed efficiency, cocoon quality, and the activities of antioxidant enzymes in midgut. The results showed that addition of NAC could significantly increase body weight, survival rate, and feed efficiency of imidacloprid poisoned silkworm larvae (P < 0.05), as well as cocoon mass, cocoon shell mass, and the ratio of cocoon shell (P < 0.05). Furthermore, it could significantly promote the activities of the antioxidant enzymes including superoxide dismutase, catalase, and glutathione peroxide in the midgut of fifth-instar larvae under imidacloprid exposure at the late stage of treatment. In addition, it also could downregulate the malondialdehyde content. The results of our findings proved that the added NAC may have some beneficial effects on protection or restoration of antioxidant balance in imidacloprid exposed larvae.

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“…A variety of transition metals are known to generate ROS through Haber-Weiss and Fenton reaction mechanisms (Knaapen et al, 2004). Multiple lines of evidence suggest that the pro-inflammatory potential of TiO 2 -NPs linked to the toxicity of this NP through the production of ROS in various cells (Hu et al, 2016;Meena et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Titanium dioxide nanoparticles induce COX-2 expression through ROS generation in human periodontal ligament cells

et al. 2019

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Titanium dioxide nanoparticles (TiO 2 -NPs) are used to improve the aesthetic of toothpaste. While TiO 2 -NPs have been used safely in toothpaste products for a long time, there haven't been studies to determine whether absorption of TiO 2 -NPs by the mucous membranes in the mouth induces pathogenic conditions. Here, we assessed whether TiO 2 -NPs induce cyclooxygenase-2 (COX-2) and investigated the molecular mechanisms underlying the pro-inflammatory effect of TiO 2 -NPs on human periodontal ligament (PDL) cells. Treatment of PDL cells with TiO 2 -NPs led to induction of both COX-2 mRNA and protein expression. TiO 2 -NPs stimulated the nuclear translocation of nuclear factor-kappaB (NF-κB) as well as its DNA binding by inducing phosphorylation and subsequent degradation of the inhibitory protein IκBα in PDL cells. TiO 2 -NPs treatment resulted in rapid activation of extracellular signal-regulated kinase (ERK)1/2 and Akt, which could be upstream of NF-κB. Treatment of PDL cells with both the MEK1/2 inhibitor U0126 and the PI3K inhibitor LY294002 strongly attenuated TiO 2 -NPs-induced activation of NF-κB, and also the expression of COX-2. PDL cells treated with TiO 2 -NPs exhibited increased accumulation of intracellular reactive oxygen species (ROS). Pretreatment of cells with ROS scavenger N-acetyl cysteine (NAC) abrogated the stimulatory effect of TiO 2 -NPs on p65, p50, and COX-2 expression. In conclusion, ROS, concomitantly overproduced by TiO 2 -NPs, induce COX-2 expression through activation of NF-κB signaling, which may contribute to the inflammatory effect of PDL cells.

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Mechanisms of TiO 2 NPs-induced phoxim metabolism in silkworm ( Bombyx mori ) fat body

Cited by 24 publications

References 35 publications

Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Knockdown of Broad-Complex Gene Expression of Bombyx mori by Oligopyrrole Carboxamides Enhances Silk Production

Preventive effects of N‐acetyl‐l‐cysteine against imidacloprid intoxication on Bombyx mori larvae

Titanium dioxide nanoparticles induce COX-2 expression through ROS generation in human periodontal ligament cells

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