Mechanisms underlying the attachment and spreading of human osteoblasts: From transient interactions to focal adhesions on vitronectin-grafted bioactive surfaces

Brun, Paola; Scorzeto, Michele; Vassanelli, Stefano; Castagliuolo, Ignazio; Palù, Giorgio; Ghezzo, Francesca; Messina, Grazia M. L.; Iucci, Giovanna; Battaglia, Valentina; Bagno, Andrea; Polzonetti, Giovanni; Marletta, Giovanni; Dettin, Monica

doi:10.1016/j.actbio.2012.12.018

Cited by 42 publications

(44 citation statements)

References 29 publications

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“…[4a, 16] Theh erein demonstrated results underline this positive effect of HBP, since cell count, size,and survival are increased compared to the platform MP.E ven better results could be achieved by coating Ti with MP-RGD since af urther increase in cell spreading,n umber of adhered cells and viability was determined. [20] Additive effects of RGD and HBP could also be observed testing the herein presented peptides with ap remature osteoblast-like cell line (MG-63). [17] Notably,acombination of HBP and RGD in one peptide (MP-RGD-HBP) induced the highest average cell area, statistically significant to blank Ti,f ibronectin, MP,M P-HBP,and MP-RGD,indicating acollaborative behavior.…”

Section: In Memory Of Klaus Braunmentioning

confidence: 99%

Multifunctional Coating Improves Cell Adhesion on Titanium by using Cooperatively Acting Peptides

et al. 2016

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Promotion of cell adhesion on biomaterials is crucial for the long-term success of a titanium implant. Herein a novel concept is highlighted combining very stable and affine titanium surface adhesive properties with specific cell binding moieties in one molecule. A peptide containing L-3,4-dihydroxyphenylalanine was synthesized and affinity to titanium was investigated. Modification with a cyclic RGD peptide and a heparin binding peptide (HBP) was realized by an efficient on-resin combination of Diels-Alder reaction with inverse electron demand and Cu(I) catalyzed azide-alkyne cycloaddition. The peptide was fluorescently labeled by thiol Michael addition. Conjugating the cyclic RGD and HBP in one peptide gave improved spreading, proliferation, viability, and the formation of well-developed actin cytoskeleton and focal contacts of osteoblast-like cells.

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Section: In Memory Of Klaus Braunmentioning

confidence: 99%

Multifunctional Coating Improves Cell Adhesion on Titanium by using Cooperatively Acting Peptides

et al. 2016

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“…[6][7][8] The use of bioactive coatings on implant surfaces has also been investigated. [9][10][11][12] Research on the effects of different surface properties on osseointegration should ideally follow a hierarchical approach before clinical trials. 13 New biomaterials should be tested first in vitro, then in vivo in animal models to ascertain their safety and efficacy.…”

Section: Introductionmentioning

confidence: 99%

A Novel In Vitro Technique for Assessing Dental Implant Osseointegration

Brunello

Ferroni

Berengo

et al. 2016

Tissue Engineering Part C: Methods

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By definition, osseointegration means close contact between bone and implant. Bone response is related to implant surface properties. Various surfaces have been studied and applied to improve the biological properties of the implant and thereby favor the mechanism of osseointegration. This strategy aims to promote osseointegration by means of a faster and stronger bone formation, improving stability during the healing process, and thus allowing for earlier loading of the implant. Dental implant osseointegration has so far been studied in various animal models. The development of a method based on tissue engineering for assessing the osseointegration process in vitro could prove a valid biomimetic alternative to sacrificing animals. In this study, flat cylindrical dental implants with moderately rough surfaces and machined implants were set in bovine bone blocks. Then, adipose-derived stem cells (ADSCs) were three dimensionally cultured onto these blocks in osteo-endothelial medium for up to 30 days to mimic the osseointegration process in vitro. Scanning electron microscopy (SEM) and gene expression were used to examine stem cell commitment. Mechanical pull-out tests were also performed. SEM analysis identified cells with an osteoblast morphology adhering to the surface of the implants after their removal. Gene expression analysis showed that ADSCs seeded onto the bone blocks were able to express osteoblast and endothelial markers. The implants with the moderately rough surface generated higher pull-out strengths when compared with the machined implants. Nevertheless, the pull-out test values were higher for implants placed in bone blocks with ADSCs than for those set in scaffolds without stem cells. Our results demonstrate the validity of the method adopted and its potential for use in the in vitro assessment of the biological behavior of dental implant surfaces.

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“…There are four key steps by which the biomaterial substrate determines cell fate: (1) biomaterial design, (2) protein adsorption on surface, (3) cell adhesion and signal transduction and (4) cell fate determination and can induce the reorganisation of actin microfilaments and promote cell adhesion and spreading, which in turn modulates cell behaviour such as cell shape and migration (Scotchford et al 2003;Howlett et al 1994). As a result, many studies have attempted to increase the deposition of fibronectin and vitronectin to improve the attachment, growth and osteoblastic differentiation of osteoprogenitor cells (Tran et al 2012;Brun et al 2013;Alves et al 2008;Woo et al 2007b). For example, nanoporous titanium surfaces have been designed to specifically increase the adsorption of fibronectin and vitronectin, which promoted osteoblast attachment and proliferation (Rivera-Chacon et al 2013).…”

Section: Protein Adsorptionmentioning

confidence: 99%

Bone-Biomimetic Biomaterial and Cell Fate Determination

Zreiqat

2014

Mechanical Engineering Series

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Despite its remarkable capacity to undergo self-repair, bone tissue cannot regenerate across critical-sized defects, and their successful reconstruction remains a major clinical challenge. Current treatment options are limited and often associated with a high incidence of complications, which may result in non-union or re-fracture. There is a great and growing need for alternative techniques to replace, restore or regenerate damaged or diseased bone. Biomaterials-based bone tissue engineering via the use of synthetic bone substitutes represents a particularly promising alternative, which circumvents the drawbacks of conventional treatments. To achieve successful reconstructive outcomes, synthetic bone substitutes need to be biocompatible and provide necessary signals to osteoprogenitor cells to control downstream cell responses including adhesion, migration, proliferation and differentiation into osteoblasts. One feasible approach to develop synthetic bone substitutes with such biological properties is to mimic the innate physical and/or chemical properties of bone. In this chapter, we discuss the design aspects of bone-biomimetic biomaterials that provide the signals necessary for bone regeneration, and the underlying mechanisms by which bone-biomimetic biomaterials determine the fate of mesenchymal stem cells/osteoprogenitor cells. Protein adsorption to biomaterial surfaces and their subsequent influence on cell adhesion and intracellular signal transduction will be discussed in detail, with particular emphasis on the key molecules and signalling pathways involved in directing the osteogenic development of cells.

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Mechanisms underlying the attachment and spreading of human osteoblasts: From transient interactions to focal adhesions on vitronectin-grafted bioactive surfaces

Cited by 42 publications

References 29 publications

Multifunctional Coating Improves Cell Adhesion on Titanium by using Cooperatively Acting Peptides

Multifunctional Coating Improves Cell Adhesion on Titanium by using Cooperatively Acting Peptides

A Novel In Vitro Technique for Assessing Dental Implant Osseointegration

Bone-Biomimetic Biomaterial and Cell Fate Determination

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