Mechanisms underlying the augmentation of phenylbiguanide and capsaicin induced cardiorespiratory reflexes by Mesobuthus tamulus venom

Dutta, Abhaya; Akella, Aparna; Deshpande, Shripad B.

doi:10.1016/j.pupt.2012.07.001

Cited by 3 publications

(4 citation statements)

References 19 publications

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“…µg/kg -100 µg/kg similar cardiorespiratory values are obtained(Dutta et al, 2012). Pulmonary edema caused by the effects of scorpion venom in the body has a cardiogenic component.…”

supporting

confidence: 75%

Experimental Evaluation of the Pulmonary Edema Induced by the Venom of the Scorpion Tityus Asthenes in Rats

Amaya-Rodríguez

Patiño²,

Young³

et al. 2022

jemb

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The species Tityus asthenes has been responsible for scorpion sting deaths in Panama. Pulmonary edema is one of the main causes of death registered by scorpionism. In the present work, we determined the capacity of the venom of the scorpion Tityus asthenes to induce pulmonary edema in rats. The ability of T. asthenes venom to induce acute pulmonary edema in rats was determined using four approaches: (1) the difference in wet weight using the lung index between treated and untreated lungs; (2) histological analysis;(3) changes in pulmonary vascular permeability; and (4) total leukocyte count obtained from bronchoalveolar lavage. We found that histological sections of venom-treated lungs showed moderate pulmonary edema, and an increase in total leukocyte count compared to control samples. However, the pulmonary index and the vascular permeability of venom-treated lungs were similar to those of control samples. We conclude that the venom of T. asthenes scorpions can induce moderate pulmonary edema in rats. The experimental model was validated for future studies on the pathophysiology of pulmonary edema caused by the venom of scorpions of the genus Tityus in Panama.

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“…µg/kg -100 µg/kg similar cardiorespiratory values are obtained(Dutta et al, 2012). Pulmonary edema caused by the effects of scorpion venom in the body has a cardiogenic component.…”

supporting

confidence: 75%

Experimental Evaluation of the Pulmonary Edema Induced by the Venom of the Scorpion Tityus Asthenes in Rats

Amaya-Rodríguez

Patiño²,

Young³

et al. 2022

jemb

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“…[ 3 11 ] In a different set of experiments, MBT venom also augmented the capsaicin-induced reflex responses in pulmonary edema-independent manner. [ 4 ] Capsaicin is a nociceptive agent that acts through transient receptor potential vanilloid receptor 1 (TRPV1) receptors present on the vagal afferents. Therefore, it was proposed that direct sensitization of nociceptive vagal afferents by the inflammatory mediators released after scorpion envenomation produce the reflex augmentation of capsaicin-induced responses.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, it was proposed that direct sensitization of nociceptive vagal afferents by the inflammatory mediators released after scorpion envenomation produce the reflex augmentation of capsaicin-induced responses. [ 4 ] Morphine being an anti-nociceptive agent, it is likely that by suppressing the nociceptor activity it prevents the augmentation of the reflex response. Thus, morphine is acting in pulmonary edema-dependent or independent manners to block the augmentation of visceral reflexes elicited by PDG.…”

Section: Discussionmentioning

confidence: 99%

“…[ 3 ] Scorpion venom-induced pulmonary edema has been shown to augment the bradycardiac reflexes elicited by phenyldiguanide (PDG), phenylbiguanide (PBG), and capsaicin. [ 4 5 ] The augmentation of PDG/PBG-induced bradycardia after envenomation has been explained on the basis of increased excitability of juxta-pulmonary capillary receptors (J receptors) due to pulmonary edema and sensitization of the vagal afferents. [ 4 5 ] Further, it has been shown that blockade of pulmonary edema by aprotinin (kinin synthase inhibitor) or N-nitro L-arginine methyl ester (nitric oxide synthase inhibitor) prevents the augmentation of PDG-induced reflexes.…”

Section: Introductionmentioning

confidence: 99%

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Morphine blocks the Mesobuthus tamulus venom-induced augmentation of phenyldiguanide reflex and pulmonary edema in anesthetized rats

et al. 2016

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Objective:Pulmonary edema, a manifestation of scorpion envenomation syndrome, is attributed to cardiogenic or noncardiogenic factors. Morphine is a drug used for cardiogenic pulmonary edema and its effect on Mesobuthus tamulus (MBT) venom-induced changes is not known. Therefore, we hypothesized that morphine blocks the MBT venom-induced augmentation of phenyldiguanide (PDG) reflex and pulmonary edema.Materials and Methods:Experiments were performed on anesthetized adult female rats. Trachea and jugular vein were cannulated, and the electrocardiographic potentials were recorded by connecting needle electrodes in limb lead II configuration. PDG (10 ΅g/kg, IV, bolus injection) responses were elicited by bolus injection initially, after saline/morphine (1 mg/kg) and after injecting MBT venom (100 μg/kg). The time-response area of the PDG-induced bradycardiac response after treatment was calculated as % of the initial PDG response area. At the end of experiments, lungs were excised for determination of pulmonary water content.Results:PDG produced bradycardiac response that lasted for >60 s. MBT venom augmented the PDG reflex response by 2.5 times. In morphine pretreated group, augmentation of bradycardiac response induced by MBT venom was absent. MBT venom increased the pulmonary water content, and the increase was absent in morphine pretreated animals.Conclusion:The results reveal that morphine prevents the MBT venom-induced augmentation of PDG reflex response and pulmonary edema. Thus, morphine can be useful in scorpion envenomation syndrome associated with pulmonary edema.

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Extraction, purification, bioactivity and pharmacological effects of capsaicin: a review

Wang

Xue

Fu³

et al. 2021

Critical Reviews in Food Science and Nutrition

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Mechanisms underlying the augmentation of phenylbiguanide and capsaicin induced cardiorespiratory reflexes by Mesobuthus tamulus venom

Cited by 3 publications

References 19 publications

Experimental Evaluation of the Pulmonary Edema Induced by the Venom of the Scorpion Tityus Asthenes in Rats

Experimental Evaluation of the Pulmonary Edema Induced by the Venom of the Scorpion Tityus Asthenes in Rats

Morphine blocks the Mesobuthus tamulus venom-induced augmentation of phenyldiguanide reflex and pulmonary edema in anesthetized rats

Extraction, purification, bioactivity and pharmacological effects of capsaicin: a review

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