Mechanisms used by DNA MMR system to cope with Cadmium-induced DNA damage in plants

Wang, Hetong; Cao, Qijiang; Zhao, Qiang; Arfan, Muhammad; Wan, Liu

doi:10.1016/j.chemosphere.2019.125614

Cited by 13 publications

(3 citation statements)

References 154 publications

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“…Cell death is a stress response to Cd in plants. The excessive accumulation of reactive oxygen species (ROS) caused by Cd exacerbates DNA damage and increases cell membrane permeability and the loss of transmembrane potential (Li et al, 2003;Michelle et al, 2014;Li et al, 2017;Huybrechts et al, 2019;Wang et al, 2019b). Vacuolar rupture is a unique and important factor causing cell death in higher plants, which is mediated by vacuolar processing enzyme (VPE) (Yamada et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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Excessive cadmium (Cd) damages plants by causing cell death. The present study discusses the function of natural resistance-associated macrophage protein (NRAMP) on cell death caused by Cd in Malus hupehensis. MhNRAMP1 was isolated from M. hupehensis roots, and its protein was located in the cell membrane as a transmembrane protein characterized by hydrophobicity. MhNRAMP1 expression in the roots was induced by Cd stress and calcium (Ca) deficiency. MhNRAMP1 overexpression increased Cd concentration in yeasts and enhanced their sensitivity to Cd. Phenotypic comparisons of plants under Cd stress revealed that the growth of transgenic tobacco and apple calli overexpressing MhNRAMP1 was worse than that of the wild type (WT). The Cd 2+ influx of transgenic tobacco roots and apple calli was higher, and the recovery time of the Cd 2+ influx to a stable state in transgenic apple calli was longer than that of the WT. Cd accumulation and the percentage of apoptotic cells in transgenic lines were higher. Correspondingly, the caspase-1-like and vacuolar processing enzyme (VPE) activities and MdVPEg expression were higher in transgenic apple calli, but the expression levels of genes that inhibit cell death were lower than those in the WT under Cd stress. Moreover, the Cd translocation from the roots to leaves was increased after MhNRAMP1 overexpression, but the Cd translocation from the leaves to seeds was not affected. These results suggest that MhNRMAP1 exacerbated Cd-induced cell death, which was accomplished by mediating Cd 2+ uptake and accumulation, as well as stimulating VPE.

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Section: Introductionmentioning

confidence: 99%

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

et al. 2020

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“…Mutated S α (MutSα), Mutated S β (MutSβ), and Mutated S γ (MutSγ) are three different MutS-associated protein heterodimer complexes consisting of MSH2/MSH6, MSH2/MSH3, and MSH2/MSH7, respectively. These complexes have different mismatch recognition properties and abilities to support MMR ( Wang et al., 2020 ). In plants, Knockdown of OsMSH2 does not cause gene variants at other locations in the genome ( Karthika et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…In plants, Knockdown of OsMSH2 does not cause gene variants at other locations in the genome ( Karthika et al., 2021 ). Mutated S homologue 2 (MSH2) preferentially activates ATR to trigger DNA damage responses (DDR), including regulation of cell cycle, endoreplication, cell death, and recruitment of other DNA repair, thereby enhancing plant tolerance to cadmium ( Wang et al., 2020 ). OsMSH6 affects rice microsatellite stability and homologous recombination and plays an important role in ensuring genome stability and genetic ( Jiang et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

E2Fs co-participate in cadmium stress response through activation of MSHs during the cell cycle

Zheng

Peng

et al. 2022

Front. Plant Sci.

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Cadmium is one of the most common heavy metal contaminants found in agricultural fields. MutSα, MutSβ, and MutSγ are three different MutS-associated protein heterodimer complexes consisting of MSH2/MSH6, MSH2/MSH3, and MSH2/MSH7, respectively. These complexes have different mismatch recognition properties and abilities to support MMR. However, changes in mismatch repair genes (OsMSH2, OsMSH3, OsMSH6, and OsMSH7) of the MutS system in rice, one of the most important food crops, under cadmium stress and their association with E2Fs, the key transcription factors affecting cell cycles, are poorly evaluated. In this study, we systematically categorized six rice E2Fs and confirmed that OsMSHs were the downstream target genes of E2F using dual-luciferase reporter assays. In addition, we constructed four msh mutant rice varieties (msh2, msh3, msh6, and msh7) using the CRISPR-Cas9 technology, exposed these mutant rice seedlings to different concentrations of cadmium (0, 2, and 4 mg/L) and observed changes in their phenotype and transcriptomic profiles using RNA-Seq and qRT-PCR. We found that the difference in plant height before and after cadmium stress was more significant in mutant rice seedlings than in wild-type rice seedlings. Transcriptomic profiling and qRT-PCR quantification showed that cadmium stress specifically mobilized cell cycle-related genes ATR, CDKB2;1, MAD2, CycD5;2, CDKA;1, and OsRBR1. Furthermore, we expressed OsE2Fs in yeasts and found that heterologous E2F expression in yeast strains regulated cadmium tolerance by regulating MSHs expression. Further exploration of the underlying mechanisms revealed that cadmium stress may activate the CDKA/CYCD complex, which phosphorylates RBR proteins to release E2F, to regulate downstream MSHs expression and subsequent DNA damage repairment, thereby enhancing the response to cadmium stress.

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Unveiling the Mechanisms of DNA Damage Response and Epigenetic Modifications of Chromatin in Response to Cadmium-Mediated Phytotoxicity

Banerjee,

Roy,

Mukherjee

et al. 2024

Environmental Science and Engineering

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Mechanisms used by DNA MMR system to cope with Cadmium-induced DNA damage in plants

Cited by 13 publications

References 154 publications

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

MhNRAMP1 From Malus hupehensis Exacerbates Cell Death by Accelerating Cd Uptake in Tobacco and Apple Calli

E2Fs co-participate in cadmium stress response through activation of MSHs during the cell cycle

Unveiling the Mechanisms of DNA Damage Response and Epigenetic Modifications of Chromatin in Response to Cadmium-Mediated Phytotoxicity

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