2023
DOI: 10.1101/2023.09.01.555893
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Mechanisms used for cDNA synthesis and site-specific integration of RNA into DNA genomes by a reverse transcriptase-Cas1 fusion protein

Georg Mohr,
Jun Yao,
Seung Kuk Park
et al.

Abstract: SUMMARYReverse transcriptase-Cas1 (RT-Cas1) fusion proteins found in some CRISPR systems enable spacer acquisition from both RNA and DNA, but the mechanism of RNA spacer acquisition has remained unclear. Here, we foundMarinomonas mediterraneaRT-Cas1/Cas2 adds short 3’-DNA (dN) tails to RNA protospacers enabling their direct integration into CRISPR arrays as 3’-dN-RNA/cDNA duplexes or 3’-dN-RNAs at rates comparable to similarly configured DNAs. Reverse transcription of RNA protospacers occurs by multiple mechan… Show more

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