Mechanistic elucidation of apoptosis and cell cycle arrest induced by 5-hydroxymethylfurfural, the important role of ROS-mediated signaling pathways

Zhao, Ling; Su, Jianyu; Li, Lin; Chen, Jianping; Hu, Song-Qing; Zhang, Xia; Chen, Tianfeng

doi:10.1016/j.foodres.2014.08.051

Cited by 33 publications

(33 citation statements)

References 60 publications

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“…The interaction of 5-HMF and Keap1 through cysteine may trigger Nrf2 nuclear translocation and antioxidant gene transcription, which may be one of the mechanisms accounts for the antioxidative effect of 5-HMF. Many studies have confirmed the role of 5-HMF in antioxidation (Ding et al 2010;Wang et al 2010;Zhao et al 2014). An in vitro study systematically investigating the antioxidant activity of 5-HMF scavenging reactive oxygen species (ROS) and the expressions of myeloperoxidase (MPO), glutathione (GSH), and superoxide dismutase (SOD), suggested that the bio-chemical nature of 5-HMF may be explained by its structure (Li et al 2009).…”

Section: Discussionmentioning

confidence: 90%

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

Hong-fang

Wang

et al. 2017

Cell Stress and Chaperones

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Recent studies have shown 5-hydroxymethyl-2-furfural (5-HMF) has favorable biological effects, and its neuroprotection in a variety of neurological diseases has been noted. Our previous study showed that treatment of 5-HMF led to protection against permanent global cerebral ischemia. However, the underlying mechanisms in cerebral ischemic injury are not fully understood. This study was conducted to investigate the neuroprotective effect of 5-HMF and elucidate the nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant response element (ARE) signaling pathway mechanism in the striatum after transient global cerebral ischemia. C57BL/6 mice were subjected to bilateral common carotid artery occlusion for 20 min and sacrificed 24 h after reperfusion. 5-HMF (12 mg/kg) or an equal volume of vehicle was intraperitoneally injected 30 min before ischemia and 5 min after the onset of reperfusion. At 24 h after reperfusion, neurological function was evaluated by neurological disability status scale, locomotor activity test and inclined beam walking test. Histological injury of the striatum was observed by cresyl violet staining and terminal deoxynucleotidyl transferase (TdT)-mediated dNTP nick end labeling (TUNEL) staining. Oxidative stress was evaluated by the carbonyl groups introduced into proteins, and malondialdehyde (MDA) levels. An enzyme-linked immunosorbent assay (ELISA)-based measurement was used to detect Nrf2 DNA binding activity. Nrf2 and its downstream ARE pathway protein expression such as heme oxygenase-1, NAD (P)H:quinone oxidoreductase 1, glutamate-cysteine ligase catalytic subunit and glutamate-cysteine ligase modulatory subunit were detected by western blot. Our results showed that 5-HMF treatment significantly ameliorated neurological deficits, reduced brain water content, attenuated striatum neuronal damage, decreased the carbonyl groups and MDA levels, and activated Nrf2/ARE signaling pathway. Taken together, these results demonstrated that 5-HMF exerted significant antioxidant and neuroprotective effects following transient cerebral ischemia, possibly through the activation of the Nrf2/ARE signaling pathway.

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Section: Discussionmentioning

confidence: 90%

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

Hong-fang

Wang

et al. 2017

Cell Stress and Chaperones

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“…MTT assay is a common manner to evaluate cell survival (Zhao et al, ). Therefore, we carried out MTT assay to detect the cell activity.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of the molecular mechanism of curcumin/β‐cyclodextrin polymer inclusion complex inhibiting HepG2 cells growth

et al. 2019

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This research aimed to explore whether curcumin/β-cyclodextrin polymer (CUR/ CDP) inclusion complex caused inhibitory effect on HepG2 cells proliferation and its possible molecular mechanisms. We found that CUR/CDP inclusion complex exhibited inhibitory effects on HepG2 cells growth. To understand the underlying mechanism of how CUR/CDP inclusion complex inhibited HepG2 cells growth, we examined the proteome of HepG2 cells treated at 640 μg/ml CUR/CDP inclusion complex using proteomic approach. We found that 15 protein spots identified by MALDI-TOF/TOF MS were changed. Biological progress analysis demonstrated that protein related to cell cycle and apoptosis accounted for 33% of the detected proteins. Among these proteins, nucleophosmin (NPM1) and peroxiredoxin-6 (PRDX6) were involved in the ROS-P53 pathway. PRDX6 and NPM1 were down-regulated, thereby improving the expression level of phosphorylated p53 and ROS content, which regulated cell apoptosis. This findings provide a better understanding of CUR/CDP regulatory antitumor mechanisms. Practical applicationsCyclodextrins polymer was used to prepare the curcumin/cyclodextrins polymer inclusion complex to improve curcumin solubility and stability in our group. And we found that it showed novel anticancer activity. However, the molecular mechanisms is unclear. This research elucidates the underlying molecular mechanisms of the curcumin/cyclodextrins polymer inclusion complex-inhibited HepG2 cells growth. The inclusion complex has the potential to be a novel complex of curcumin to treat human cancers K E Y W O R D S apoptosis, curcumin/β-cyclodextrin polymer inclusion complex, HepG2 cells, proteomic analysis

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“…The search for AChE inhibitors has been biased towards alkaloids (Elgorashi and Van Staden, ; Elgorashi et al , ; Houghton et al , ; Adewusi et al , ), even though other phytochemicals such as sterols, terpenoids, oils, flavonoids and other phenolic compounds have also been suggested, and in some instances, shown to possess anti‐AChE properties (Ji and Zhang, ; Sacan and Yanardag, ). Besides alkaloids (Elgorashi et al , ; Houghton et al , ; Nair et al , ; Nair and Van Staden, ), coumarins (Kang et al , ; Fallarero et al , ; Senol et al , ; Adhami et al , ) and other compounds such as 5‐HMF (Liu et al , ; Zhao et al , ) and flavonoids (Maryam et al , ) have been shown to exhibit AChE inhibitory properties. The present study explored the main phenolic acids and flavonoid constituents in the different parts of C .…”

Section: Discussionmentioning

confidence: 99%

“…Using an in vivo model, Liu et al () have demonstrated that 5‐HMF could effectively ameliorate memory performance, improve memory impairments and enhance activity of the antioxidative defence system in experimental mice. 5‐Hydroxymethylfurfural, a furan compound with chamomile flavour, is derived from the Maillard reaction and degradation of hexoses; thus, it widely exists in various food products and plants which contain high sugar content (Zhao et al , ). Notably, 5‐HMF has been shown to exhibit other biological activities such as antioxidant, cytoprotective, antiproliferative and chemotherapeutic effects (Zhao et al , ; Liu et al , ; Zhao et al , ).…”

Section: Discussionmentioning

confidence: 99%

“…5‐Hydroxymethylfurfural, a furan compound with chamomile flavour, is derived from the Maillard reaction and degradation of hexoses; thus, it widely exists in various food products and plants which contain high sugar content (Zhao et al , ). Notably, 5‐HMF has been shown to exhibit other biological activities such as antioxidant, cytoprotective, antiproliferative and chemotherapeutic effects (Zhao et al , ; Liu et al , ; Zhao et al , ). The fact that galanthamine and rivastigmine, which are recommended drugs for AD symptomatic treatment, have a direct natural origin has justified the necessity for exploration of nature as a source of bioactive compounds, which may serve as leads or scaffolds for further chemical elucidation (Fallarero et al , ).…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical Characterization, Antibacterial, Acetylcholinesterase Inhibitory and Cytotoxic Properties of Cryptostephanus vansonii, an Endemic Amaryllid

Moyo

Aremu

Chukwujekwu

et al. 2017

Phytotherapy Research

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Cryptostephanus vansonii I. Verd., an endemic Amaryllidaceae species from Zimbabwe, was evaluated for its acetylcholinesterase (AChE) inhibitory and cytotoxicity properties using Ellman's colorimetric method and the tetrazolium-based colorimetric assay against Vero monkey kidney cells, respectively. The plant extracts were also evaluated for their antibacterial activity against five bacteria. Furthermore, phytochemical profiles of the extracts were determined using ultra-high performance liquid chromatography coupled with tandem mass spectrometry analysis. A plant part-dependent AChE inhibitory activity was observed, in the order, root > rhizome > basal leaf > leaf. Overall, C. vansonii extracts exhibited better antibacterial activity against Gram-negative compared with Gram-positive bacteria. Cytotoxic effects were not detected in Vero monkey kidney cell lines suggesting the possible absence of toxophores in C. vansonii extracts. Similar to the trend in biological activity, a distinct plant part-dependent variation in hydroxybenzoates, hydroxycinnamates and flavonoids was observed in the plant extracts. In addition, 5-hydroxymetylfurfural and eucomic acid were detected in the different plant parts of C. vansonii. The results of the present study provide valuable AChE inhibition activity, toxicological and phytochemical profiles of C. vansonii. Further studies on isolation of bioactive compounds and their subsequent evaluation in other pharmacological and toxicological model systems are required. Copyright © 2017 John Wiley & Sons, Ltd.

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Mechanistic elucidation of apoptosis and cell cycle arrest induced by 5-hydroxymethylfurfural, the important role of ROS-mediated signaling pathways

Cited by 33 publications

References 60 publications

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

5-HMF attenuates striatum oxidative damage via Nrf2/ARE signaling pathway following transient global cerebral ischemia

Proteomic analysis of the molecular mechanism of curcumin/β‐cyclodextrin polymer inclusion complex inhibiting HepG2 cells growth

Phytochemical Characterization, Antibacterial, Acetylcholinesterase Inhibitory and Cytotoxic Properties of Cryptostephanus vansonii, an Endemic Amaryllid

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