Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing

Patel, Satish; Srivastava, Shikha; Singh, Manju Rawat; Singh, Deependra

doi:10.1016/j.biopha.2019.108615

Cited by 659 publications

(515 citation statements)

References 155 publications

Supporting

Mentioning

503

Contrasting

Unclassified

Order By: Relevance

“…When O 2 consumption is more substantial than molecular oxygen release, the hypoxia process happens. The occurrence of local wound hypoxia is related to the reduced level of circulating blood due to narrowed vessels or / and to the modification of hemoglobin due to hyperglycemia [55,56].…”

Section: Importance Of Oxygen Release In Wounds Treatmentmentioning

confidence: 99%

The importance of oxygen release in diabetic foot ulcer wounds

2019

Bioengineering Int

View full text Add to dashboard Cite

Oxygen is the essential element required for proper physiological function of cells, tissues and organs within healthy human body. Thanks to its intricate structure, the skin provides a multiprotective barrier against traumatic and non-traumatic injuries, but also a complex and successful self-healing process of the affected tissue. In the particular case of chronic skin wounds, such as diabetic foot ulcer wounds, there is an immediate demand to develop alternative procedures that prevent infection, speed up healing and eliminate any disrupting factor that may interfere with the therapeutic process. Given the importance of oxygen during wound healing cascade, impressive attention was oriented towards the fabrication of oxygen-releasing wound dressings.

show abstract

Section: Importance Of Oxygen Release In Wounds Treatmentmentioning

confidence: 99%

The importance of oxygen release in diabetic foot ulcer wounds

2019

Bioengineering Int

View full text Add to dashboard Cite

show abstract

“…Leg or foot ulcers are the most common type of wounds in patients with diabetes. 1 Diabetic cutaneous ulcers are the result of a combination of vascular diseases, neuropathy, and infection. 2 Among them, vascular diseases can lead to microvascular injuries or microcirculation disorders to varying degrees, which further cause distal ischemia and severe tissue damage.…”

Section: Introductionmentioning

confidence: 99%

<p>miRNA-221-3p in Endothelial Progenitor Cell-Derived Exosomes Accelerates Skin Wound Healing in Diabetic Mice</p>

Bai

Cao

et al. 2020

DMSO

View full text Add to dashboard Cite

Background: Patients with diabetic cutaneous ulcers experience financial burden and a lower quality of life and life expectancy. Endothelial progenitor cell (EPC)-derived exosomes facilitate skin wound healing by positively modulating vascular endothelial cell function. Exosomes play their important regulatory role through microRNA (miRNA). We explored the potential role and molecular mechanisms of miRNA in EPC-derived exosome healing of diabetic skin wounds. Methods: Exosomes were isolated from the media of EPCs derived from mice bone marrow. High-throughput sequencing was used to detect the expression of exosome miRNA, and miRNA target genes were predicted using online databases. A diabetic mouse skin wound model was established, and wounds were treated with exosomes, miRNA-221-3p, or phosphate-buffered saline. Results: Exosomes from EPCs accelerated skin wound healing in both control and diabetic mice. High-throughput sequencing showed that miRNA-221-3p was highly expressed in EPCderived exosomes. Skin wound healing in control and diabetic mice was significantly enhanced by EPC-derived exosomes and miRNA-221-3p administration. Immunohistochemical analyses showed that EPC-derived exosomes and miRNA-221-3p increased protein expression levels of the angiogenesis-related factors VEGF, CD31 and cell proliferation marker Ki67. Bioinformatics analyses indicated that miRNA-221-3p may be involved in the AGE-RAGE signaling pathway in diabetic complications, cell cycle, and the p53 signaling pathway. Conclusion: We concluded that miRNA-221-3p is one of the high-expressed miRNAs in EPC-derived exosomes and promoted skin wound healing in diabetic mice. The finding uncovers the molecular mechanism of EPC-derived exosomes and provides a potential novel approach to the clinical treatment of diabetic skin wounds.

show abstract

“…During this phase, fibroblasts migrate to the wound and synthesize collagen, and epithelial cells proliferate and close the wound. The formation of new blood vessels by angiogenesis plays a key role in this process by providing cells with nutrients and oxygen necessary for the developing tissue [4]. When the robust proliferation and extracellular matrix (ECM) synthesis is over, wound healing enters the final remodeling phase, in which vascular density returns to the normal level and ECM architecture approaches that of the normal tissue.…”

Section: Introductionmentioning

confidence: 99%

Nano-in-Micro Dual Delivery Platform for Chronic Wound Healing Applications

et al. 2020

View full text Add to dashboard Cite

Here, we developed a combinatorial delivery platform for chronic wound healing applications. A microfluidic system was utilized to form a series of biopolymer-based microparticles with enhanced affinity to encapsulate and deliver vascular endothelial growth factor (VEGF). Presence of heparin into the structure can significantly increase the encapsulation efficiency up to 95% and lower the release rate of encapsulated VEGF. Our in vitro results demonstrated that sustained release of VEGF from microparticles can promote capillary network formation and sprouting of endothelial cells in 2D and 3D microenvironments. These engineered microparticles can also encapsulate antibiotic-loaded nanoparticles to offer a dual delivery system able to fight bacterial infection while promoting angiogenesis. We believe this highly tunable drug delivery platform can be used alone or in combination with other wound care products to improve the wound healing process and promote tissue regeneration.

show abstract

Mechanistic insight into diabetic wounds: Pathogenesis, molecular targets and treatment strategies to pace wound healing

Cited by 659 publications

References 155 publications

The importance of oxygen release in diabetic foot ulcer wounds

The importance of oxygen release in diabetic foot ulcer wounds

<p>miRNA-221-3p in Endothelial Progenitor Cell-Derived Exosomes Accelerates Skin Wound Healing in Diabetic Mice</p>

Nano-in-Micro Dual Delivery Platform for Chronic Wound Healing Applications

Contact Info

Product

Resources

About