Mechanistic insights into the protective role of eugenol against stress-induced reproductive dysfunction in female rat model

Helmy, Hanan; Sadik, Nermin Abdel Hamid; Badawy, Laila; Sayed, Noha H.

doi:10.1016/j.cbi.2022.110181

Cited by 6 publications

(7 citation statements)

References 63 publications

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“…[45] A study conducted in female rat, reported that peroral administration of eugenol 50 mg/kg for a duration of 8 weeks attenuated stressinduced elevated corticosterone and LH levels with a decrease in estradiol and antimullerian hormone during the reproductive age. [26] Eugenol at a high dose (400 mg/kg) increased the diabetesinduced decrease in tubular diameter and the number of the seminiferous tubules. Further, diabetes significantly decreased the spermatogenesis as measured by Johnson's score, and eugenol at higher dose (400 mg/kg) restored the same.…”

Section: Discussionmentioning

confidence: 94%

“…[ 45 ] A study conducted in female rat, reported that peroral administration of eugenol 50 mg/kg for a duration of 8 weeks attenuated stress‐induced elevated corticosterone and LH levels with a decrease in estradiol and antimullerian hormone during the reproductive age. [ 26 ]…”

Section: Discussionmentioning

confidence: 99%

“…Eugenol was prepared freshly by mixing with olive oil and administered orally using oral gavage. [26,27] T1DM was induced by a single i.p. dose of 55 mg/kg, freshly prepared STZ in cold citrate buffer (pH 4.5).…”

Section: Methodsmentioning

confidence: 99%

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Protective role of eugenol against diabetes‐induced oxidative stress, DNA damage, and apoptosis in rat testes

Chilukoti,

Sahu,

Jena

2023

J Biochem & Molecular Tox

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Diabetes mellitus, a metabolic disorder alters gonadal development and spermatogenesis, reactive oxygen species production, DNA damage, and apoptosis, which subsequently lead to male subfertility. Eugenol is an antioxidant, traditionally used as medication for digestive disorders and antioxidant therapy, decrease transport of glucose from GIT to systemic circulation. This experiment was aimed to decipher cellular and molecular insights of eugenol in protecting diabetic germ cells in rats. Rats were assigned randomly into five groups: control, eugenol control (Eugenol 400; EUG), diabetic (DIA), diabetic + eugenol 100 (DIA + EUG 100), and diabetic + eugenol 400 (DIA + EUG 400). EUG 400 and DIA + EUG 400 groups received 400 mg/kg eugenol orally. DIA + EUG 100 group received 100 mg/kg eugenol. Treatment was conducted for 4 weeks. Type 1 diabetes was induced by injecting a single i.p. dose of streptozotocin (55 mg/kg). Morphometric, biochemical, sperm parameters, oxidative stress, hormonal levels, histopathology, and fibrosis in the testis and epididymis, were evaluated. DNA damage was evaluated using halo and comet assays; DNA fragmentation and apoptosis using TUNEL assay. Eugenol treatment significantly normalized biochemical parameters, reduced MDA while increased albumin and GSH levels in diabetes. Eugenol significantly increased sperm numbers, motility and attenuated abnormal sperm head morphology in diabetes. Moreover, eugenol significantly reversed diabetes‐induced cellular damages, altered spermatogenesis, and collagen deposition in testis and epididymis. It also significantly attenuated diabetes‐associated DNA breaks and apoptosis. These findings suggest that 4 weeks treatment with 400 mg/kg of eugenol could be beneficial for diabetic patients to prevent subfertility.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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Protective role of eugenol against diabetes‐induced oxidative stress, DNA damage, and apoptosis in rat testes

Chilukoti,

Sahu,

Jena

2023

J Biochem & Molecular Tox

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“…In addition, other conditions where oxidative stress and genotoxicity are fundamental for the development of diseases, benefit from the biological activity of eugenol such as diabetes [77] multidrug-resistant bacterial infections by Klebsiella [78] stress-induced reproductive system disorders [79] kidney damage induced by CCl4- [80], as well as the biological activity of gum arabic, such as in dyslipidemia that occurs as a result of sickle cell anemia, chronic kidney disease [81] protection hepatica after exposure to aflatoxins [66] (and cardiac protection in ischemia and reperfusion injuries [82] It is important to stress that the antioxidant and anticarcinogenic effects of gum arabic and eugenol described previously in the literature only involved the isolated use of the substances. Here we describe the novel synergistic effects of the two substances administered together.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis

de Oliveira Ramos Melo,

da Costa Marques,

Neto

et al. 2024

FNS

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Spices are defined as any aromatic condiment of plant origin used to alter the flavor and aroma of foods. Besides flavor and aroma, many spices have antioxidant activity, mainly related to the presence in cloves of phenolic compounds, such as flavonoids, terpenoids and eugenol. In turn, the most common uses of gum arabic are in the form of powder for addition to soft drink syrups, cuisine and baked goods, specifically to stabilize the texture of products, increase the viscosity of liquids and promote the leavening of baked products (e.g., cakes). Both eugenol, extracted from cloves, and gum arabic, extracted from the hardened sap of two species of the Acacia tree, are dietary constituents routinely consumed virtually throughout the world. Both of them are also widely used medicinally to inhibit oxidative stress and genotoxicity. The prevention arm of the study included groups: Ia,

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“…Moreover, the HPA (hypothalamic-pituitary-adrenal) axis deactivation concludes the decline of stress-associated overexpression of CRHR-1 (corticotropin-releasing hormone receptor-1). Hence, it can be concluded that eugenol administration could have a positive impact on females struggling with stress-induced reproductive disorders, thereby affecting fertility [ 102 ].…”

Section: Mechanism Of Action Of Selected Plant Constituents For Treat...mentioning

confidence: 99%

Phytochemicals: Alternative for Infertility Treatment and Associated Conditions

Chorosho,

Malik,

Panesar

et al. 2023

Oxidative Medicine and Cellular Longevity

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Infertility and obstetric complications have become global health issues in the past few years. Infertility is defined as the inability of a couple to conceive even after twelve months or more of regular and unprotected intercourse. According to WHO data published in the year 2020, 186 million people have infertility globally. Factors leading to infertility are variable in both males and females. But some common factors include smoking, alcohol consumption, obesity, and stress. Various synthetic drugs and treatment options are available that are effective in treating infertility, but their prolonged usage produces various unwanted adverse effects like hot flashes, mood swings, headaches, and weight gain. In extreme cases, these may also lead to the development of anxiety and depression. Herbal remedies have gained a lot of popularity over the years, and people’s inclination toward them has increased all over the world. The prime reason is that these show significant therapeutic efficacy and have fewer side effects. The therapeutic efficacy of plants can be attributed to the presence of diverse phytochemical classes of constituents like alkaloids, flavonoids, and volatile oils. These secondary metabolites, or phytomolecules, can be used to develop herbal formulations. The review highlights the applications and mechanisms of action of various phytochemicals for treating infertility. Also, it focuses on the various future prospects associated with it.

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Mechanistic insights into the protective role of eugenol against stress-induced reproductive dysfunction in female rat model

Cited by 6 publications

References 63 publications

Protective role of eugenol against diabetes‐induced oxidative stress, DNA damage, and apoptosis in rat testes

Protective role of eugenol against diabetes‐induced oxidative stress, DNA damage, and apoptosis in rat testes

Enhancement of the Antigenotoxic and Antioxidant Actions of Eugenol from Spice Clove and the Stabilizer Gum Arabic on Colorectal Carcinogenesis

Phytochemicals: Alternative for Infertility Treatment and Associated Conditions

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