The effective development of low permeability heavy oil reservoirs is crucial for tapping into unconventional resources. High-pressure CO 2 flooding offers numerous benefits, including reducing crude oil viscosity, enhancing oil fluidity, and decreasing interfacial tension, leading to improved heavy oil production. Additionally, CO 2 can be sequestered in the formation, contributing to carbon emission reduction. However, analyzing the migration and storage effects of CO 2 in low permeability heavy oil reservoirs is challenging. In this study, high-pressure and hightemperature (50 MPa,100 °C) microfluidic experiments were designed and carried out, and the CO 2 flooding characteristics and storage efficiency were studied. The distribution of the remaining oil and mechanisms of CO 2 sequestration under various displacement speeds and injection pressures were analyzed. The results demonstrated that CO 2 flooding achieved significantly higher recovery rates compared to high-pressure water flooding, with increments of 13.89, 21.53, and 23.09% at oil displacement pressures of 30, 40, and 50 MPa, respectively. The CO 2 burial efficiency also showed substantial improvements, reaching 23.55, 34.01, and 37.13% under the corresponding conditions. Furthermore, a higher porosity facilitated CO 2 migration, providing more space and migration channels for CO 2 flow. By examining CO 2 −oil interactions, the study elucidated CO 2 migration patterns and burial efficiency under different displacement modes.