Mechanistic study of the adjuvant effect of biodegradable nanoparticles in mucosal vaccination

Slütter, Bram; Plapied, Laurence; Fiévez, Virginie; Sande, Maria Alonso; Rieux, Anne des; Schneider, Yves‐Jacques; Riet, Elly van; Jiskoot, Wim; Préat, Véronique

doi:10.1016/j.jconrel.2009.05.011

Cited by 182 publications

(108 citation statements)

References 45 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…OVA-loaded PLGA nanoparticles were prepared by double emulsion with solvent evaporation method as previously reported with modifications [25]. Briefly, 75 μl OVA (20 mg/ml) in PBS was dispersed in 1 ml PLGA (25 mg/ml) in ethyl acetate by a Branson sonifier 250 (Danbury, USA) for 15 s with a power of 20 W. To prepare anionic OVA-loaded PLGA nanoparticles (anPLGA-OVA), the obtained water-inoil emulsion was emulsified with 2 ml 2% (w/v) PVA with the sonifier (15 s, 20 W) to get a water-in-oil-in-water double emulsion.…”

Section: Preparation Of Plga Nanoparticlesmentioning

confidence: 99%

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Groot

Mönkäre

et al. 2017

Journal of Controlled Release

View full text Add to dashboard Cite

A B S T R A C TThe skin is an attractive organ for immunization due to the presence of a large number of epidermal and dermal antigen-presenting cells. Hollow microneedles allow for precise and non-invasive intradermal delivery of vaccines. In this study, ovalbumin (OVA)-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles with and without TLR3 agonist poly(I:C) were prepared and administered intradermally by hollow microneedles. The capacity of the PLGA nanoparticles to induce a cytotoxic T cell response, contributing to protection against intracellular pathogens, was examined. We show that a single injection of OVA-loaded PLGA nanoparticles, compared to soluble OVA, primed both adoptively transferred antigen-specific naïve transgenic CD8 + and CD4 + T cells with markedly high efficiency. Applying a triple immunization protocol, PLGA nanoparticles primed also endogenous OVA-specific CD8 + T cells. Immune response, following immunization with in particular anionic PLGA nanoparticles co-encapsulated with OVA and poly(I:C), provided protection against a recombinant strain of the intracellular bacterium Listeria monocytogenes, secreting OVA. Taken together, we show that PLGA nanoparticle formulation is an excellent delivery system for protein antigen into the skin and that protective cellular immune responses can be induced using hollow microneedles for intradermal immunizations.

show abstract

Section: Preparation Of Plga Nanoparticlesmentioning

confidence: 99%

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Groot

Mönkäre

et al. 2017

Journal of Controlled Release

View full text Add to dashboard Cite

show abstract

“…The next step in the development of a superior PLGA is considered to be the addition of a functional block to PEG-b-PLGA, allowing for the covalent OVA is commonly used as a model antigen/protein in murine animal studies investigating protein drug delivery with hydrophobic micro-or nanoparticles; its use in the current work therefore allowed for comparison of the obtained data to literature (Reddy et al, 2007;Slütter et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

Rietscher

Czaplewska

Majdanski

et al. 2016

International Journal of Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…One way consists of 70 encapsulating the antigen inside liposomes [17,18] or inside organic biodegradable polymers, such as poly(lactic acid) (PLA), poly(lactic-co-glycolic acid) (PLGA), [19][20][21][22][23][24] disulfide cross-linked polyacrylates, [25] or polysaccharides, such as pullulan [26] and chitosan. [27][28][29] This way, the particles can easily reach cytosol, where they are degraded, thus releasing the antigen. The other approach consists of attaching the antigen (covalently or not) to the functionalized surface of 75 biocompatible and non-biodegradable particulate materials, such as lecithin NPs, [30] gold NPs,[31-33] quantum dots, [34] or MPs/NPs based on diverse organic polymers like polystyrene, [35,36] polypropylene sulfide, [37] or polyacrylate.…”

Section: Introductionmentioning

confidence: 99%

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Parra

Abad‐Somovilla

Mercader

et al. 2013

Journal of Controlled Release

View full text Add to dashboard Cite

Carbon nanotubes (CNTs) are nanomaterials with interesting emerging applications. Their 25 properties makes CNTs excellent candidates for use as new nanovehicles in drug delivery, immunization and diagnostics. In the current study, we assessed the immune-response-amplifying properties of CNTs to haptens by using azoxystrobin, the first developed strobilurin fungicide, as a model analyte. An azoxystrobin derivative bearing a carboxylated spacer arm (hapten AZc6) was covalently coupled to bovine serum albumin (BSA), and the resulting BSA-AZc6 conjugate was 30 covalently linked to four functionalized CNTs of different shapes and sizes, varying in diameter and length. These four types of CNT-based constructs were obtained using efficient, fast, and easy functionalization procedures based on microwave-assisted chemistry. New Zealand rabbits and BALB/c mice were immunized with BSA-AZc6 alone and with the four CNT-BSA-AZc6 constructs, both with and without Freund's adjuvant. The IgG-type antibody responses were assessed 35 in terms of the titer and affinity, paying special attention to the relationship between the immune response and the size and shape of the employed CNTs. Immunization with CNT-BSA-AZc6 resulted in enhanced titers and excellent affinities for azoxystrobin. More important, remarkable IgG responses were obtained even in the absence of an adjuvant, thus proving the self-adjuvanting capability of CNTs. Immunogens were able to produce strong anti-azoxystrobin immune responses 40 in rabbits even when administered at a BSA-AZc6 conjugate dose as low as 0.05 g. The short and thick CNT-BSA-AZc6 construct produced the best antibody response under all tested conditions.

show abstract

Mechanistic study of the adjuvant effect of biodegradable nanoparticles in mucosal vaccination

Cited by 182 publications

References 45 publications

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Hollow microneedle-mediated intradermal delivery of model vaccine antigen-loaded PLGA nanoparticles elicits protective T cell-mediated immunity to an intracellular bacterium

Impact of PEG and PEG- b -PAGE modified PLGA on nanoparticle formation, protein loading and release

Carbon nanotube-protein carriers enhance size-dependent self-adjuvant antibody response to haptens

Contact Info

Product

Resources

About