Mechanistic Study of the Photodynamic Inactivation of
            <i>Candida albicans</i>
            by a Cationic Porphyrin

Lambrechts, Saskia A. G.; Aalders, Maurice C. G.; Marle, J. van

doi:10.1128/aac.49.5.2026-2034.2005

Cited by 157 publications

(145 citation statements)

References 43 publications

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“…[17][18][19][20][21][22][23][24] The results of the present investigation demonstrated significant reduction of CFU/mL of C. albicans from tongues of mice after 400, 500, and 1000 mg/L of Photogem associated with LED light (305 J/cm 2 ) (455 and 630 nm). There is only one report describing a dose-dependent photoeradication of C. albicans in an immunodeficient murine model.…”

Section: Discussionsupporting

confidence: 56%

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Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

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Objective. In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated. Study design. Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10 7 CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm 2 ) at 455 or 630 nm (n ϭ 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P Ͻ .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction. Results. PDT resulted in a significant reduction in C. albicans recovered from the tongue (P Ͻ .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa. Conclusion. PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.

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Section: Discussionsupporting

confidence: 56%

“…are susceptible to photoinactivation. [17][18][19][20][21][22][23][24] Usually, dyes (toluidine blue and methylene blue) and porphyrins are used as PS combined with red laser light. However, light sources with simpler technology and lower cost than lasers, such as light-emitting diodes (LED), have been successfully applied in PDT.…”

mentioning

confidence: 99%

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Mima

Pavarina

Dovigo

et al. 2010

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

141

130

View full text Add to dashboard Cite

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“…Elas são fáceis de cultivar e podem ser usadas em estudos que são complicados demais para serem feitos com células superiores. Leveduras, tais como Saccharomyces marxianus têm sido usadas como organismos-modelo para acessar o dano celular por PDI em células eucarióticas 2 .…”

Section: Fungos E Levedurasunclassified

“…Na presença de oxigênio encontrado nas células, o fotossensibilizador (FS) ativado pode reagir com moléculas na sua vizinhança por transferência de elétrons ou hidrogênio, levando à produção de radicais livres (reação do tipo I) ou por transferência de energia ao oxigênio (reação do tipo II), levando à produção de oxigênio singlete. Ambos os caminhos podem levar à morte celular e à destruição do tecido doente 2,3 . O oxigênio singlete reage com quase todos os componentes celulares uma vez que os compostos orgânicos insaturados são, de forma geral, suscetíveis à ação de 1 O 2 .…”

Section: Introductionunclassified

Inativação fotodinâmica de microrganismos

Perussi¹

2007

Quím. Nova

113

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“…The 1 O2 reacts with cellular components, since the unsaturated organic compounds are generally susceptible to the action of 1 O2. The first barrier to 1 O2 is the cell membrane, and, because it has unsaturated lipids in its composition, this can be damaged during the described process, causing cellular inviability 14,15 . The interest in efficient fungicide treatments has been increasing due to the increasing number of fungal pathogens responsible for nosocomial infections or opportunistic mycoses in immunocompromised patients.…”

Section: When In Contact Withmentioning

confidence: 99%

In vitro reduction of pathogenic sporothrix schenckii fungus by photodynamic therapy

Guirado¹,

Navarro²,

Rossoni³

et al. 2017

Mundo da Saúde

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A esporotricose é uma doença que afeta os vasos linfáticos, pele e alguns órgãos internos. A maioria dos casos se apresenta como uma micose subaguda à crônica, provocada por fungos do complexo Sporothrix schenckii, bastante comuns em regiões tropicais. O objetivo deste estudo foi avaliar a suscetibilidade aos efeitos da inativação fotodinâmica em células leveduriformes de fungos do complexo Sporothrix schenckii. Para tal, as células viáveis foram separadas em quatro grupos, sendo estes: grupo irradiado com fotossensibilizador (L+F+); grupo irradiado sem fotossensilizador (L+F-), grupo não irradiado com fotossensibilizador (L-F+); e grupo não irradiado sem fotossensibilizador (L-F-). A concentração do fotossensibilizador azul de metileno utilizada foi de 0,1 mg/mL, e a dosagem do laser de Arseneto de Gálio Alumínio foi de 26,3 J/cm2. Em seguida, foi realizada a contagem das unidades formadoras de colônias (UFCs) em cada grupo. Como principal resultado, verificou-se que o grupo irradiado com fotossensibilizador (L+F+) foi o único que não apresentou crescimento de UFCs. Dessa forma, concluiu-se que fungos do complexo Sporothrix schenckii podem ser inativados com o uso da terapia fotodinâmica. Palavras-chave:Esporotricose. Sporothrix. Azul de Metileno. Lasers.Sporotrichosis is a disease that affects the lymph vessels, skin and some internal organs. Most cases are presented as a subacute chronic mycosis caused by the Sporothrix schenckii fungus; fairly common in tropical regions. The aim of this study was to evaluate the susceptibility of Sporothrix schenckii yeast cells to the effects of photodynamic inactivation. For this, the viable cells were separated into four groups: irradiated with photosensitizer group (L+F+); irradiated without photosensitizer group (L+F-), without irradiation and with photosensitizer group (L-F+); and without irradiation and without photosensitizer group (L-F-). The methylene blue photosensitizer concentration used was 0.1 mg/mL, and the Aluminum Gallium Arsenide laser dose was 26.3 J/cm2. Then, counting of colony forming units (CFUs) was performed in each group. The main result was that the irradiated group with photosensitizer (L+F+) was the one that showed no growth of CFUs. Thus, it was concluded that Sporothrix schenckii can be inactivated by use of photodynamic therapy

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Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Cited by 157 publications

References 43 publications

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Susceptibility of Candida albicans to photodynamic therapy in a murine model of oral candidosis

Inativação fotodinâmica de microrganismos

In vitro reduction of pathogenic sporothrix schenckii fungus by photodynamic therapy

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