Mechanistic understanding of nanoparticles’ interactions with extracellular matrix: the cell and immune system

Engin, Ayşe Başak; Nikitovic, Dragana; Neagu, Monica; Henrich‐Noack, Petra; Docea, Anca Oana; Shtilman, Mikhail I.; Golokhvast, Kirill S.; Tsatsakis, Aristidis

doi:10.1186/s12989-017-0199-z

Cited by 192 publications

(136 citation statements)

References 133 publications

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“…The interaction of NPs with the complex structure of the ECM is now well established 49,50 . It has been postulated that the filtering effect of the ECM (due to its dense structure) and its distinct charge properties may hinder the passage of large-sized NPs but, at the same time, the movement of very small NPs (less than about 20 nm) may not be hampered and may, thus, exhibit higher toxic potential 51 . The type of interaction of NP with cell membrane may ultimately decide the intracellular trafficking, localization and compartmentalization, cellular retention, etc.…”

Section: Discussionmentioning

confidence: 99%

Accumulation and trafficking of zinc oxide nanoparticles in an invertebrate model, Bombyx mori, with insights on their effects on immuno-competent cells

Mir

Qamar

Qadir

et al. 2020

Sci Rep

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Zinc oxide nanoparticles (Zno nps) are used in many applications; however, their interactions with cells, immune cells in particular, and potential health risk(s) are not fully known. In this manuscript, we have demonstrated the potential of ZnO NPs to cross the gut barrier in an invertebrate model, Bombyx mori, and that they can reach the hemolymph where they interact with and/or are taken up by immunecompetent cells resulting in various toxic responses like decline in hemocyte viability, ROS generation, morphological alterations, apoptotic cell death, etc. exposure to these nps also resulted in alteration of hemocyte dynamics including an immediate increase in THC, possibly due to the release of these hemocytes either from enhanced rate of cell divisions or from attached hemocyte populations, and decline in percentage of prohemocytes and increase in percentage of two professional phagocytes, i.e., granulocytes and plasmatocytes, possibly due to the differentiation of prohemocytes into phagocytes in response to a perceived immune challenge posed by these NPs. Taken together, our data suggest that ZnO NPs have the potential to cross gut barrier and cause various toxic effects that could reverse and the insects could return to normal physiological states as there is restoration and repair of various systems and their affected pathways following the clearance of these NPs from the insect body. Our study also indicates that B. mori has the potential to serve as an effective alternate animal model for biosafety, environmental monitoring and screening of NPs, particularly to evaluate their interactions with invertebrate immune system.The nanotechnology has developed rapidly in the present world and engineered nanoparticles (NPs) have found tremendous applications in diverse products like cosmetics, pharmaceuticals, food industry products, paints, electronics, clothing, etc. 1-3 Such large-scale use of nanoparticles has raised concerns with regard to their impacts on human health, biodiversity, environment and ecosystems; more so because of their small dimensions and other unique properties. Since precise cellular mechanism(s) of nanoparticle interaction with biological systems are mostly unknown, nanotoxicology demands comprehensive assessments of various interactions between NPs and biological systems based upon their various exposure routes. The gravity of the environmental concerns regarding the use and disposal of NPs has attracted a plethora of studies to assess their toxicity, both in vivo and in vitro 4 . Exposure to various NPs has been reported to cause oxidative stress, cytotoxicity, DNA damage, apoptosis, necrosis, genotoxicity, aberrant mitochondrial function and reduced photosynthesis in different cell lines [5][6][7][8][9][10][11] . Various in vivo studies in a wide spectrum of organisms have linked NP exposure to various toxic effects like developmental abnormalities in zebra fish embryos 12,13 , decline in vertebrate lung function 14 , interaction with sea urchin immune cells 15 , increased mutagenesis...

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Section: Discussionmentioning

confidence: 99%

Accumulation and trafficking of zinc oxide nanoparticles in an invertebrate model, Bombyx mori, with insights on their effects on immuno-competent cells

Mir

Qamar

Qadir

et al. 2020

Sci Rep

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“…Strategic treatments eliminate tangible factors that contribute to weaken the immune system. Some examples of such factors, based on existing studies, include titanium dioxide, air pollution, chlorpyrifos, aluminum sulfate, prenatal alcohol exposure, ZnO, oil fly ash, and many others (3)(4)(5)(6)(7)(8)(9). Also the real life exposure scenario in which we are exposed continuously to combination of stressors influences in the long term the immune system and the response of the organism to the viral stressors (10)(11)(12)(13)(14).…”

Section: Towards Effective Covid-19 Vaccines: Updates Perspectives Amentioning

confidence: 99%

Towards effective COVID‑19 vaccines: Updates, perspectives and challenges (Review)

et al. 2020

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In the current context of the pandemic triggered by SARS-cOV-2, the immunization of the population through vaccination is recognized as a public health priority. In the case of SARS-cOV-2, the genetic sequencing was done quickly, in one month. Since then, worldwide research has focused on obtaining a vaccine. This has a major economic impact because new technological platforms and advanced genetic engineering procedures are required to obtain a cOVId-19 vaccine. The most difficult scientific challenge for this future vaccine obtained in the laboratory is the proof of clinical safety and efficacy. The biggest challenge of manufacturing is the construction and validation of production platforms capable of making the vaccine on a large scale.

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“…Nanoparticles are subject to steric and adhesive interactions with ECM constituents and cellular surfaces, as well as hydrodynamic interactions brought about by the narrow confines of the ECS. The physical properties of the ECM are strongly dictated by the density of the biopolymers that make up the ECM, which influence the effective ECM mesh size (Engin et al, 2017). In the brain, the effect of steric constraints brought about by the ECS and ECM has been demonstrated previously.…”

Section: Recent Work By Sigal Et Al Used a Stochastic Optical Reconsmentioning

confidence: 97%

Multiple particle tracking detects changes in brain extracellular matrix structure and predicts neurodevelopmental age

McKenna

Shackelford

Pontes

et al. 2020

Preprint

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Brain extracellular matrix (ECM) structure mediates many aspects of neuronal function. Probing changes in ECM structure could provide insights into aging and neurological disease. Herein, we demonstrate the ability to characterize changes in brain ECM structure using multiple particle tracking (MPT). MPT was carried out in organotypic rat brain slices to detect induced and naturally occurring changes in ECM structure. Induced degradation of neural ECM led to a significant increase in nanoparticle diffusive ability in the brain extracellular space. For structural changes that occur naturally during development, an inverse relationship existed between age and nanoparticle diffusion. Using the age-dependent dataset, we applied extreme gradient boosting (XGBoost) to generate models capable of classifying nanoparticle trajectories.Collectively, this work demonstrates the utility of MPT combined with machine learning for measuring changes in brain ECM structure and predicting associated complex features such as developmental age.

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Mechanistic understanding of nanoparticles’ interactions with extracellular matrix: the cell and immune system

Cited by 192 publications

References 133 publications

Accumulation and trafficking of zinc oxide nanoparticles in an invertebrate model, Bombyx mori, with insights on their effects on immuno-competent cells

Accumulation and trafficking of zinc oxide nanoparticles in an invertebrate model, Bombyx mori, with insights on their effects on immuno-competent cells

Towards effective COVID‑19 vaccines: Updates, perspectives and challenges (Review)

Multiple particle tracking detects changes in brain extracellular matrix structure and predicts neurodevelopmental age

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