Mechano‐Dynamic Analysis of the Bactericidal Activity of Bioinspired Moth‐Eye Nanopatterned Surfaces (Adv. Mater. Interfaces 23/2022)

Alameda, María Teresa; Osorio, Manuel; Rodríguez, Isabel

doi:10.1002/admi.202270127

Cited by 2 publications

(1 citation statement)

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“…A well‐defined array of nanocones with a mean height of 350 nm, width of 80 nm at the tip, and an aspect ratio of 4.3, arranged on a dense hexagonal lattice of 250 nm pitch, was observed. It was previously shown that these same nanostructures fabricated on poly (methyl methacrylate) are able to kill up to 40% of Gram‐negative bacteria after they were in contact for 1–7 h [14a,15] . We aimed at monitoring the real‐time response of E. coli in contact with the nanostructured topographies at shorter times by using Min oscillations as a reporter, and compare it with the response to contact with a flat OrmoComp surface ( Figure A).…”

Section: Resultsmentioning

confidence: 99%

Real‐Time Imaging of the Mechanobactericidal Action of Colloidal Nanomaterials and Nanostructured Topographies

et al. 2023

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Mechanobactericidal nanomaterials, such as low‐dimensional nanoparticles in suspension or high‐aspect‐ratio nanofabricated topographies, rely on their mechanical or physical interactions with bacteria and are promising antimicrobial strategies that overcome bacterial resistance to classical antibiotics. However, the underlying killing mechanisms are poorly understood, given the challenges associated with the real‐time characterization of the mechanical interaction in a biologically relevant environment. Indeed, different death mechanisms have been proposed depending on the magnitude of the interaction forces. Herein, the real‐time and single‐cell response of bacteria to weak mechanical interactions with nanostructured topographies and “nanodarts,” exemplified by flowing single‐walled carbon nanotubes, is investigated. To that end, an advanced reporting strategy is used to follow sublethal physiological effects on bacteria upon contact with these materials. With this method, it is estimated that the contact time at which the initial stages of bacterial death occur is in the order of a few tens of minutes. This information contributes to a full understanding of the complex mechanisms of mechanically induced bacterial death.

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Section: Resultsmentioning

confidence: 99%