Mechanobiology of force transduction in dermal tissue

Silver, Frederick H.; Siperko, Lorraine M.; Seehra, Gurinder P.

doi:10.1034/j.1600-0846.2003.00358.x

Cited by 335 publications

(348 citation statements)

References 197 publications

(283 reference statements)

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“…The genes of focus in these studies are tenascin-C and collagen fibril-associated type XII collagen. Tenascin-C, which is not normally expressed in adults, changes its expression patterns during embryogenesis and regenerative processes and appears under pathological conditions and adjacent to contracting wounds (Mackie et al, 1988;Silver et al, 2003). Tensile loading has been shown to control tenascin-C mRNA expression in chicken skin fibroblasts.…”

Section: Regulation Of Gene Expression In Fibroblasts By Mechanical Lmentioning

confidence: 99%

Mechanoregulation of gene expression in fibroblasts

Wang

Thampatty

Lin

et al. 2007

Gene

227

187

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Mechanical loads placed on connective tissues alter gene expression in fibroblasts through mechanotransduction mechanisms by which cells convert mechanical signals into cellular biological events, such as gene expression of extracellular matrix components (e.g., collagen). This mechanical regulation of ECM gene expression affords maintenance of connective tissue homeostasis. However, mechanical loads can also interfere with homeostatic cellular gene expression and consequently cause the pathogenesis of connective tissue diseases such as tendinopathy and osteoarthritis. Therefore, the regulation of gene expression by mechanical loads is closely related to connective tissue physiology and pathology. This article reviews the effects of various mechanical loading conditions on gene regulation in fibroblasts and discusses several mechanotransduction mechanisms. Future research directions in mechanoregulation of gene expression are also suggested.

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Section: Regulation Of Gene Expression In Fibroblasts By Mechanical Lmentioning

confidence: 99%

Mechanoregulation of gene expression in fibroblasts

Wang

Thampatty

Lin

et al. 2007

Gene

227

187

View full text Add to dashboard Cite

show abstract

“…Plasticity and molecular remodeling are key mechanical features of ordinary connective tissue in which collagen and other ECM molecules can stretch, slip, and undergo stable reorganization relative to each other [1]. As a result, the tissue is in a sense tunable according to the mechanical needs of the body.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast mechanics in 3D collagen matrices☆

Rhee

Grinnell²

2007

Advanced Drug Delivery Reviews

166

115

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Connective tissues provide mechanical support and frameworks for the other tissues of the body. Type 1 collagen is the major protein component of ordinary connective tissue, and fibroblasts are the cell type primarily responsible for its biosynthesis and remodeling. Research on fibroblasts interacting with collagen matrices explores all four quadrants of cell mechanics: pro-migratory vs. pro-contractile growth factor environments on one axis; high tension vs. low tension cell-matrix interactions on the other. The dendritic fibroblast -probably equivalent to the resting tissue fibroblast -can be observed only in the low tension quadrant and generally has not been appreciated from research on cells incubated with planar culture surfaces. Fibroblasts in the low tension quadrant require microtubules for formation of dendritic extensions, whereas fibroblasts in the high tension quadrant require microtubules for polarization but not for spreading. Ruffling of dendritic extensions rather than their overall protrusion or retraction provides the mechanism for remodeling of floating collagen matrices, and floating matrix remodeling likely reflects a model of tissue mechanical homeostasis.

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“…Three-dimensional (3D) matrices prepared with type I collagen exhibit mechanical properties that resemble connective tissue (Barocas et al, 1995;Wakatsuki et al, 2000;Roeder et al, 2002;Silver et al, 2002;Ahlfors and Billiar, 2007). Unlike ECM-coated material surfaces, fibroblasts can mechanically remodel collagen matrices both locally and globally (Brown et al, 1998;Tomasek et al, 2002;Grinnell, 2003;Petroll, 2004; Tranquillo, 1999Such mechanical remodeling of connective tissue ECM is believed to be important for tissue homeostasis (Silver et al, 2002;Wiig et al, 2003;Goldsmith et al, 2004;Langevin et al, 2004), aging (Varani et al, 2004), repair (Tonnesen et al, 2000Tomasek et al, 2002;Grinnell, 2003), fibrosis (Eckes et al, 2000;Desmouliere et al, 2005), and tumorigenesis (Beacham and Cukierman, 2005;Gaggioli et al, 2007;Yamada and Cukierman, 2007).Cells interacting with 3D collagen matrices exhibit distinct patterns of cell signaling (Cukierman et al, 2002;Wozniak et al, 2003;Beningo et al, 2004;Rhee et al, 2007) and increased plasticity of cell migration (Sahai and Marshall, 2003;Shreiber et al, 2003;Friedl, 2004;Even-Ram and Yamada, 2005;Zaman et al, 2006;Wolf et al, 2007). We have been studying human foreskin fibroblast migration in nested collagen matrices .…”

mentioning

confidence: 99%

Collagen Fibril Flow and Tissue Translocation Coupled to Fibroblast Migration in 3D Collagen Matrices

Miron-Mendoza

Seemann

Grinnell

2008

MBoC

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In nested collagen matrices, human fibroblasts migrate from cell-containing dermal equivalents into surrounding cell-free outer matrices. Time-lapse microscopy showed that in addition to cell migration, collagen fibril flow occurred in the outer matrix toward the interface with the dermal equivalent. Features of this flow suggested that it depends on the same cell motile machinery that normally results in cell migration. Collagen fibril flow was capable of producing large-scale tissue translocation as shown by closure of a ϳ1-mm gap between paired dermal equivalents in floating, nested collagen matrices. Our findings demonstrate that when fibroblasts interact with collagen matrices, tractional force exerted by the cells can couple to matrix translocation as well as to cell migration. INTRODUCTIONCell migration depends on the multistep process of cell extension, adhesion, exertion of backward tractional force, and tail retraction (Lauffenburger and Horwitz, 1996;Mitchison and Cramer, 1996;Galbraith and Sheetz, 1997;Beningo et al., 2001;Ridley et al., 2003). The extensive body of research underlying the multistep model is based on studies with tissue cells on extracellular matrix (ECM)-coated planar surfaces including rigid materials such as glass or plastic coverslips as well as flexible materials such as polyacrylamide. On ECM-coated planar surfaces, cells can modulate their cytoskeletal function and adhesion strength in response to surface mechanics (Balaban et al., 2001;Discher et al., 2005;Ingber, 2006;Vogel and Sheetz, 2006). However, adsorbed or covalently attached ECM molecules tend to be held in register with each other with little capacity to undergo cell-mediated mechanical and molecular reorganization.Type 1 collagen is the major protein component of fibrous connective tissues. These connective tissues provide mechanical support and frameworks throughout the body, and fibroblasts are the cell type primarily responsible for their biosynthesis and remodeling. Three-dimensional (3D) matrices prepared with type I collagen exhibit mechanical properties that resemble connective tissue (Barocas et al., 1995;Wakatsuki et al., 2000;Roeder et al., 2002;Silver et al., 2002;Ahlfors and Billiar, 2007). Unlike ECM-coated material surfaces, fibroblasts can mechanically remodel collagen matrices both locally and globally (Brown et al., 1998;Tomasek et al., 2002;Grinnell, 2003;Petroll, 2004; Tranquillo, 1999Such mechanical remodeling of connective tissue ECM is believed to be important for tissue homeostasis (Silver et al., 2002;Wiig et al., 2003;Goldsmith et al., 2004;Langevin et al., 2004), aging (Varani et al., 2004), repair (Tonnesen et al., 2000Tomasek et al., 2002;Grinnell, 2003), fibrosis (Eckes et al., 2000;Desmouliere et al., 2005), and tumorigenesis (Beacham and Cukierman, 2005;Gaggioli et al., 2007;Yamada and Cukierman, 2007).Cells interacting with 3D collagen matrices exhibit distinct patterns of cell signaling (Cukierman et al., 2002;Wozniak et al., 2003;Beningo et al., 2004;Rhee et al., 2007) and increa...

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Mechanobiology of force transduction in dermal tissue

Cited by 335 publications

References 197 publications

Mechanoregulation of gene expression in fibroblasts

Mechanoregulation of gene expression in fibroblasts

Fibroblast mechanics in 3D collagen matrices☆

Collagen Fibril Flow and Tissue Translocation Coupled to Fibroblast Migration in 3D Collagen Matrices

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