Mechanoenzymatic reactions with whole cell transaminases: shaken, not stirred

Abstract

Mechanochemical reactions have emerged in recent years as a green synthetic method because reactions can be performed more rapidly and using less solvent than traditional synthetic approaches. To date, very...

Mechanoenzymatic reactions for the hydrolysis of PET

“…17,[36][37][38][39][41][42][43][44]48 It was also considered that lysing the cells in situ to release the enzymes via mechanical milling might be benecial. 48 For polymer-assisted grinding it has been stated that having more matter inside the jars can promote reactions, so inert additives in the form of cell debris might also aid the reaction. 43 RAging cycles were employed to mitigate against enzyme denaturation due to increased temperatures from mechanical forces.…”

“…This was attempted as enhancements have been seen in other hydrolytic reactions and more recently transaminase reactions with whole cells. 17,[36][37][38][39][41][42][43][44]48 It was also considered that lysing the cells in situ to release the enzymes via mechanical milling might be benecial. 48 For polymer-assisted grinding it has been stated that having more matter inside the jars can promote reactions, so inert additives in the form of cell debris might also aid the reaction.…”

“…Action of the ball mill can effectively lyse the cells in situ, and in a low bulk solvent environment provides high enzyme concentrations, which together with assisted grinding can promote the reactions. 48,49 In this study, for the rst time, whole cell PETase mechanoenzymatic hydrolytic approaches and the effect of RAging on the breakdown of BHET and PET have been investigated (Scheme 1). This revealed signicant advantages over traditional large bulk solvent based hydrolytic enzymatic reactions, with a marked increase in hydrolytic capability of a range of PET hydrolases.…”

“…Action of the ball mill can effectively lyse the cells in situ , and in a low bulk solvent environment provides high enzyme concentrations, which together with assisted grinding can promote the reactions. 48,49…”

“…Significantly increased yields of primary and secondary amines from aldehydes and ketones were generated compared to traditional solution reactions. 48 The use of whole cells has the benefit of simplifying the enzyme preparation process, removing the need for cell lysis and enzyme purification. Action of the ball mill can effectively lyse the cells in situ , and in a low bulk solvent environment provides high enzyme concentrations, which together with assisted grinding can promote the reactions.…”