Mechanoregulatory role of TRPV4 in prenatal skeletal development

Abstract

Biophysical cues are essential for guiding skeletal development, but the mechanisms underlying the mechanical regulation of cartilage and bone formation are unknown. TRPV4 is a cell membrane ion channel responsible for transducing mechanical stimuli as a means of regulating skeletal cell homeostatic processes. Dysregulation of TRPV4 is associated with several skeletal developmental pathologies, indicating its involvement in cartilage and bone development, potentially in a mechanosensing capacity. In this study, we test the hypothesis that mechanically mediated prenatal skeletogenesis depends on TRPV4 activity. We first validate a novel model where we establish that dynamically loading embryonic mouse hindlimb explants cultured ex vivo promotes joint cartilage growth and morphogenesis, but not diaphyseal mineralization. We next reveal that TRPV4 protein expression is mechanically regulated and spatially localized to patterns of high biophysical stimuli in the femoral condyles of cultured limbs. Finally, we demonstrate that TRPV4 activity is crucial for the mechanical regulation of joint cartilage growth and shape, mediated via the control of cell proliferation and matrix biosynthesis, indicating a mechanism by which mechanical loading could direct morphogenesis during joint formation. We conclude that the regulatory pathways initiated by TRPV4 mechanotransduction are essential for the for the cartilage response to physical stimuli during skeletal development. Therefore, TRPV4 may be a valuable target for the development of therapeutic skeletal disease modifying drugs and developmentally-inspired tissue engineering strategies for skeletal repair.

YAP and TAZ couple osteoblast precursor mobilization to angiogenesis and mechanoregulated bone development

“…We developed a bioreactor system for ex vivo mechanical stimulation of explanted fetal limbs ( 22 ). Pharmacologic inhibition shows that the ion channel Trpv4( 40 ) mediates skeletal mechanosensing, but the mechanotransductive signaling mediators are unknown. YAP and TAZ mediate mechanotransduction in other embryonic tissues ( 41 ), and are dysregulated in a mouse model of fetal akinesia( 42 ).…”