Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix

Kim, In Gul; Gil, Chang-Hyun; Seo, Joseph; Park, Soon‐Jung; Subbiah, Ramesh; Jung, Taek-Hee; Kim, Jong Soo; Jeong, Young-Hoon; Chung, Hyung-Min; Lee, Jong Ho; Lee, Man Ryul; Moon, Sung-Hwan; Park, Kwideok

doi:10.1016/j.biomaterials.2017.10.016

Cited by 39 publications

(38 citation statements)

References 27 publications

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“…Moreover, the surface stiffness also affects the proliferation of stem cells [94]; a previous study has shown that hydrogels with very soft modulus (~10 Pa) decreased cell proliferation and differentiation [95]. In addition, stiffness is an important factor to maintain the survival rate for stem cells; studies have shown that stem cell on the matrix with a stiffness of 200 Pa survived more than 90% compared to 80% in cultures (100 Pa) [96]. Another study showed that the hardness of 2.5 MPa increased pluripotency [97].…”

Section: Stiffness Effectsmentioning

confidence: 99%

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Xing

Zhou

et al. 2019

Stem Cells International

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It is well known that stem cells reside within tissue engineering functional microenvironments that physically localize them and direct their stem cell fate. Recent efforts in the development of more complex and engineered scaffold technologies, together with new understanding of stem cell behavior in vitro, have provided a new impetus to study regulation and directing stem cell fate. A variety of tissue engineering technologies have been developed to regulate the fate of stem cells. Traditional methods to change the fate of stem cells are adding growth factors or some signaling pathways. In recent years, many studies have revealed that the geometrical microenvironment played an essential role in regulating the fate of stem cells, and the physical factors of scaffolds including mechanical properties, pore sizes, porosity, surface stiffness, three-dimensional structures, and mechanical stimulation may affect the fate of stem cells. Chemical factors such as cell-adhesive ligands and exogenous growth factors would also regulate the fate of stem cells. Understanding how these physical and chemical cues affect the fate of stem cells is essential for building more complex and controlled scaffolds for directing stem cell fate.

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Section: Stiffness Effectsmentioning

confidence: 99%

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Xing

Zhou

et al. 2019

Stem Cells International

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“…Pluripotency is, by definition, the main feature of iPSCs. Thus making them very adaptable and very sensitive to external stimuli [ 205 , 206 , 207 , 208 ]. Consequently, the design of in vitro experiments is paramount, requiring a deep deliberation regarding the definition of the right balance between simplicity and thoroughness.…”

Section: Disease Modellingmentioning

confidence: 99%

“…iPSC potency and their capability to undergo differentiation into mature somatic cells is supported by biochemical and mechanical stimuli, mediated by extracellular matrix (ECM) and neighbouring cells. Cell–matrix adhesion and cell–cell interaction have a demonstrated effect on cell morphology and the expression of pluripotent genes [ 212 ] governed by mechanotransduction pathways [ 206 , 207 ] and several in vitro models have been proposed to test mechanosensing dependent responses. In general, culturing iPSCs on substrates coated with native ECM components significantly increases cell maturation levels.…”

Section: Disease Modellingmentioning

confidence: 99%

Dystrophin Cardiomyopathies: Clinical Management, Molecular Pathogenesis and Evolution towards Precision Medicine

D’Amario

Gowran

Canonico

et al. 2018

JCM

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Duchenne’s muscular dystrophy is an X-linked neuromuscular disease that manifests as muscle atrophy and cardiomyopathy in young boys. However, a considerable percentage of carrier females are often diagnosed with cardiomyopathy at an advanced stage. Existing therapy is not disease-specific and has limited effect, thus many patients and symptomatic carrier females prematurely die due to heart failure. Early detection is one of the major challenges that muscular dystrophy patients, carrier females, family members and, research and medical teams face in the complex course of dystrophic cardiomyopathy management. Despite the widespread adoption of advanced imaging modalities such as cardiac magnetic resonance, there is much scope for refining the diagnosis and treatment of dystrophic cardiomyopathy. This comprehensive review will focus on the pertinent clinical aspects of cardiac disease in muscular dystrophy while also providing a detailed consideration of the known and developing concepts in the pathophysiology of muscular dystrophy and forthcoming therapeutic options.

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“…The effects of stiffness observed in mESCs are not translated to hPSCs. There is limited consensus on the effects of stiffness, with some groups finding little to no influence of stiffness on pluripotency (Keung et al, 2012;Maldonado et al, 2015;Przybyla et al, 2016;Price et al, 2017) and others finding the opposite (Musah et al, 2012;Sun et al, 2012;Kim et al, 2018;Sung et al, 2018). Nonetheless, observations made by several groups imply that stiffer substrates are more suitable for hPSC expansion.…”

Section: Stiffness Of Substrate On Psc Expansionmentioning

confidence: 99%

Emerging Methods for Enhancing Pluripotent Stem Cell Expansion

Chan

Rizwan

Yim

2020

Front. Cell Dev. Biol.

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Pluripotent stem cells (PSCs) have great potential to revolutionize the fields of tissue engineering and regenerative medicine as well as stem cell therapeutics. However, the end goal of using PSCs for therapeutic use remains distant due to limitations in current PSC production. Conventional methods for PSC expansion have limited potential to be scaled up to produce the number of cells required for the end-goal of therapeutic use due to xenogenic components, high cost or low efficiency. In this mini review, we explore novel methods and emerging technologies of improving PSC expansion: the use of the two-dimensional mechanobiological strategies of topography and stiffness and the use of three-dimensional (3D) expansion methods including encapsulation, microcarrierbased culture, and suspension culture. Additionally, we discuss the limitations of conventional PSC expansion methods as well as the challenges in implementing non-conventional methods.

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Mechanotransduction of human pluripotent stem cells cultivated on tunable cell-derived extracellular matrix

Cited by 39 publications

References 27 publications

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Regulation and Directing Stem Cell Fate by Tissue Engineering Functional Microenvironments: Scaffold Physical and Chemical Cues

Dystrophin Cardiomyopathies: Clinical Management, Molecular Pathogenesis and Evolution towards Precision Medicine

Emerging Methods for Enhancing Pluripotent Stem Cell Expansion

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