Mechanotropism of single cells adhering to elastic substrates subject to exogenous forces

Palumbo, Stefania; Carotenuto, Angelo Rosario; Cutolo, A.; Deseri, Luca; Pugno, Nicola; Fraldi, Massimiliano

doi:10.1016/j.jmps.2021.104475

Cited by 4 publications

(2 citation statements)

References 83 publications

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“…Apart from their ability to exert dynamic forces, cells are mechanosensitive to dynamic stimuli and have shown to demonstrate (i) modified cytoskeletal structure and its fluidization at high stretch rates; , (ii) mechanoptosis; (iii) mechanotropism; (iv) altered ECM expression and remodeling; − (v) increase culture cell growth rate by nanokicking; ,, (vi) torsional and translational vibrations of the cell nucleus; (vii) synchronization of beating cardiomyocytes, , (viii) higher cellular viability at specific frequency and amplitude ranges, and (ix) altered collective cell migration …”

Section: Introductionmentioning

confidence: 99%

“…40,48−50 Interestingly, myosin motor attachment and detachment rates are in the order of 35 Hz and 350 Hz, respectively; and (iv) tensegrity vibrations are high frequency natural modes arising due to cytoskeletal tension. 51−53 Apart from their ability to exert dynamic forces, cells are mechanosensitive 54 to dynamic stimuli and have shown to demonstrate (i) modified cytoskeletal structure and its fluidization at high stretch rates; 55,56 (ii) mechanoptosis; 57 (iii) mechanotropism; 58 (iv) altered ECM expression and remodeling; 59−65 (v) increase culture cell growth rate by nanokicking; 47,66,67 (vi) torsional and translational vibrations of the cell nucleus; 53 (vii) synchronization of beating cardiomyocytes, 10,15 (viii) higher cellular viability at specific frequency and amplitude ranges, 68 and (ix) altered collective cell migration. 69 As for the substrate that carries these perturbations, the prestressed ECM fibers, 40,70,71 being a constituent of a hydrogel with both storage and loss moduli, are expected to more significantly overdamp transverse waves and mainly allow underdamped longitudinal stretch waves.…”

Section: ■ Introductionmentioning

confidence: 99%

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Does the Extracellular Matrix Support Cell–Cell Communication by Elastic Wave Packets?

Panchenko

Tchaicheeyan

Berinskii

et al. 2022

ACS Biomater. Sci. Eng.

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The extracellular matrix (ECM) is a fibrous network supporting biological cells and provides them a medium for interaction. Cells modify the ECM by applying traction forces, and these forces can propagate to long ranges and establish a mechanism of mechanical communication between neighboring cells. Previous studies have mainly focused on analysis of static force transmission across the ECM. In this study, we explore the plausibility of dynamic mechanical interaction, expressed as vibrations or abrupt fluctuations, giving rise to elastic waves propagating along ECM fibers. We use a numerical mass-spring model to simulate the longitudinal and transversal waves propagating along a single ECM fiber and across a 2D random fiber network. The elastic waves are induced by an active contracting cell (signaler) and received by a passive neighboring cell (receiver). We show that dynamic wave propagation may amplify the signal at the receiver end and support up to an order of magnitude stronger mechanical cues and longer-ranged communication relative to static transmission. Also, we report an optimal impulse duration corresponding to the most effective transmission, as well as extreme fast impulses, in which the waves are encaged around the active cell and do not reach the neighboring cell, possibly due to the Anderson localization effect. Finally, we also demonstrate that extracellular fluid viscosity reduces, but still allows, dynamic propagation along embedded ECM fibers. Our results motivate future biological experiments in mechanobiology to investigate, on the one hand, the mechanosensitivity of cells to dynamic forces traveling and guided by the ECM and, on the other hand, the impact of ECM architecture and remodeling on dynamic force transmission and its spectral filtering, dispersion, and decay.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Does the Extracellular Matrix Support Cell–Cell Communication by Elastic Wave Packets?

Panchenko

Tchaicheeyan

Berinskii

et al. 2022

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

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Mechanics-Based Models to Predict the Alignment of Cells on a Cyclically Stretched Substrate

Giverso,

Lucci,

Preziosi

2024

SEMA SIMAI Springer Series

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Actomyosin contractility and buckling of microtubules in nucleation, growth and disassembling of focal adhesions

Palumbo

Benvenuti

Fraldi

2022

Biomech Model Mechanobiol

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Building up and maintenance of cytoskeletal structure in living cells are force-dependent processes involving a dynamic chain of polymerization and depolymerization events, which are also at the basis of cells’ remodelling and locomotion. All these phenomena develop by establishing cell–matrix interfaces made of protein complexes, known as focal adhesions, which govern mechanosensing and mechanotransduction mechanisms mediated by stress transmission between cell interior and external environment. Within this framework, by starting from a work by Cao et al. (Biophys J 109:1807–1817, 2015), we here investigate the role played by actomyosin contractility of stress fibres in nucleation, growth and disassembling of focal adhesions. In particular, we propose a tensegrity model of an adherent cell incorporating nonlinear elasticity and unstable behaviours, which provides a new kinematical interpretation of cellular contractile forces and describes how stress fibres, microtubules and adhesion plaques interact mechanobiologically. The results confirm some experimental evidences and suggest how the actomyosin contraction level could be exploited by cells to actively control their adhesion, eventually triggering cytoskeleton reconfigurations and migration processes observed in both physiological conditions and diseases.

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Mechanotropism of single cells adhering to elastic substrates subject to exogenous forces

Cited by 4 publications

References 83 publications

Does the Extracellular Matrix Support Cell–Cell Communication by Elastic Wave Packets?

Does the Extracellular Matrix Support Cell–Cell Communication by Elastic Wave Packets?

Mechanics-Based Models to Predict the Alignment of Cells on a Cyclically Stretched Substrate

Actomyosin contractility and buckling of microtubules in nucleation, growth and disassembling of focal adhesions

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