Meconium microbiome and its relation to neonatal growth and head circumference catch-up in preterm infants

Nutricionist, Ana Carolina Terrazzan; Procianoy, Renato S.; Roesch, Luiz Fernando Würdig; Corso, Andrea; Dobbler, Priscila Caroline Thiago; Silveira, Rita de Cássia dos Santos

doi:10.1371/journal.pone.0238632

Cited by 17 publications

(21 citation statements)

References 57 publications

(75 reference statements)

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“…This study shows the development and diversity of the gut microbiome in 24 samples derived from the feces samples of eight preterm neonates born at CMH, Jakarta, at 3 different time points in early life. The analysis of gut microbiome development over the first week based on alpha and beta diversity in terms of relative abundance showed that for the 8 subjects providing 24 feces samples at the three time points, i.e., meconium, day 4 feces, and day 7 feces, Proteobacteria and Firmicutes were dominant at the phylum level, while at the genus level, unidentified Enterobacteriaceae were most abundant, as has been reported widely 1 , 6 , 7 , 9 , 10 , 29 – 34 . This result is similar to our previous study on cultivable bacteria from meconium from neonates born in CMH 9 .…”

Section: Discussionsupporting

confidence: 64%

Metagenomic profiles of the early life microbiome of Indonesian inpatient neonates and their influence on clinical characteristics

Amandito

Malik

Rohsiswatmo

2022

Sci Rep

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Determining the initial normal neonatal gut microbiome is challenging. The debate regarding the sterile fetal environment is still ongoing. Therefore, studying and comparing normal and dysbiotic microbiomes requires the elucidation of both the fetal and infant microbiomes. Factors influencing the normal microbiome also include regional and genetic factors specific to different countries. Determining the normal microbiome population in our center and their association with the clinical conditions of infants is helpful as a tool for both the prevention and treatment of related diseases during neonatal care. Here, we employed metagenomic sequencing to characterize meconium and the subsequent early-life gut microbiome of preterm neonates in Jakarta, Indonesia. Microbiome diversity and complexity was higher in the meconium and on day 4 than on day 7. At the genus level, the most abundant genus overall was unidentified Enterobacteriaceae, with meconium samples dominated by Ureaplasma, day 4 fecal samples dominated by Staphylococcus, and day 7 samples dominated by Clostridiales, while at the phylum level the most abundant was Proteobacteria and Firmicutes. Perinatal factors of PROM and mother’s diet influenced the meconium microbiome, while day 4 and day 7 microbiome was associated with bacteremia and early administration of antibiotics. One of our sample sets was derived from triplets, and they had varying diversity despite being triplets. These data are valuable for understanding the formation of a healthy microbiome specific to neonates and devising a strategy to improve both the gut health and related clinical outcomes of the neonate.

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Section: Discussionsupporting

confidence: 64%

Metagenomic profiles of the early life microbiome of Indonesian inpatient neonates and their influence on clinical characteristics

Amandito

Malik

Rohsiswatmo

2022

Sci Rep

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“…Another study in Brazil, compared microbiome diversity in meconium between infants with early HC catch-up growth (<6 months) and infants with late HC catch-up growth (>6 months). They reported greater microbial diversity and a higher abundance of Acinetobacter in meconium from infants with late HC catch-up growth that was independent of infant weight ( Terrazzan Nutricionist et al, 2020 ). Some of these results were consistent with our findings, as we also found HC to be associated with human milk alpha-diversity in late lactation.…”

Section: Discussionmentioning

confidence: 99%

Human milk microbial species are associated with infant head-circumference during early and late lactation in Guatemalan mother-infant dyads

Ajeeb

González

Solomons³

et al. 2022

Front. Microbiol.

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Human milk contains abundant commensal bacteria that colonize and establish the infant’s gut microbiome but the association between the milk microbiome and head circumference during infancy has not been explored. For this cross-sectional study, head-circumference-for-age-z-scores (HCAZ) of vaginally delivered breastfed infants were collected from 62 unrelated Mam-Mayan mothers living in eight remote rural communities in the Western Highlands of Guatemala during two stages of lactation, ‘early’ (6–46 days postpartum, n = 29) or ‘late’ (109–184 days postpartum, n = 33). At each stage of lactation, infants were divided into HCAZ ≥ −1 SD (early: n = 18; late: n = 14) and HCAZ < −1 SD (early: n = 11; late: n = 19). Milk microbiome communities were assessed using 16S ribosomal RNA gene sequencing and DESeq2 was used to compare the differential abundance (DA) of human milk microbiota with infant HCAZ subgroups at both stages of lactations. A total of 503 ESVs annotated 256 putative species across the 64 human milk samples. Alpha-diversity using Chao index uncovered a difference in microbial community richness between HCAZ ≥ −1 SD and HCAZ < −1 SD groups at late lactation (p = 0.045) but not at early lactation. In contrast, Canonical Analysis of Principal Coordinates identified significant differences between HCAZ ≥ −1 SD and HCAZ < −1 SD at both stages of lactation (p = 0.003); moreover, 26 milk microbial taxa differed in relative abundance (FDR < 0.05) between HCAZ ≥ −1 SD and HCAZ < −1 SD, with 13 differentially abundant at each lactation stage. Most species in the HCAZ ≥ −1 SD group were Streptococcus species from the Firmicutes phylum which are considered human colonizers associated with human milk whereas the HCAZ < −1 SD group at late lactation had more differentially abundant taxa associated with environmentally and ‘potentially opportunistic’ species belonging to the Actinobacteria genus. These findings suggest possible associations between brain growth of breastfed infants and the milk microbiome during lactation. Importantly, these data provide the first evidence of cross talk between the human milk microbiome and the infant brain that requires further investigation.

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“…Congenital infections are a group of diseases that are present from the fetal into the early postnatal stage of life, which have been found to have adverse effects on metabolic, microbiome, and neurological development in the neonate ( Surtees et al, 1990 ; Mechtler and Kinkel, 1993 ; Terrazzan Nutricionist et al, 2020 ). TORCH is a common acronym used to refer to a set of these infections: (T)oxoplasmosis, (O)ther agents such as syphilis and parvovirus B19, Rubella, (C)ytomegalovirus infection (CMV), and (H)erpes simplex virus (HSV) ( Macedo-da-Silva et al, 2020 ; Jaan and Rajnik, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Congenital Infection Influence on Early Brain Development Through the Gut-Brain Axis

2022

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The mechanisms by which various pathogens cause congenital infections have been studied extensively, aiding in the understanding of the detrimental effects these infections can have on fetal/neonatal neurological development. Recent studies have focused on the gut-brain axis as pivotal in neurodevelopment, with congenital infections causing substantial disruptions. There remains controversy surrounding the purported sterility of the placenta as well as concerns regarding the effects of exposure to antibiotics used during pregnancy on neonatal microbiome development and how early exposure to microbes or antibiotics can shape the gut-brain axis. Long-term neurodevelopmental consequences, such as autism spectrum disorder, attention deficit hyperactivity disorder, and cerebral palsy, may be attributable, in part, to early life infection and changes in the immature gut microbiome. The goal of this review is thus to critically evaluate the current evidence related to early life infection affecting neurodevelopment through the gut-brain axis.

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Meconium microbiome and its relation to neonatal growth and head circumference catch-up in preterm infants

Cited by 17 publications

References 57 publications

Metagenomic profiles of the early life microbiome of Indonesian inpatient neonates and their influence on clinical characteristics

Metagenomic profiles of the early life microbiome of Indonesian inpatient neonates and their influence on clinical characteristics

Human milk microbial species are associated with infant head-circumference during early and late lactation in Guatemalan mother-infant dyads

Congenital Infection Influence on Early Brain Development Through the Gut-Brain Axis

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