Meconopsis horridula Hook. f. &amp; Thomson extract and its alkaloid oleracein E exert cardioprotective effects against acute myocardial ischaemic injury in mice

Zhao, Feng; Bai, Ruifeng; Li, Junjun; Feng, Xiao; Jiao, Shungang; Wuken, Shana; Ge, Fu-Xing; Zhang, Qian; Zhou, Xiaosu; Tu, Pengfei; Chai, Xingyun

doi:10.1016/j.jep.2020.112893

Cited by 20 publications

(6 citation statements)

References 24 publications

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“…Ensuring the blood supply recovery of the ischemic myocardial tissue is an important goal in the efforts to prevent or alleviate MI/RI. As an essential component of traditional Chinese medicines, TTMs have advantages in terms of their efficacy and fewer side effects due to their many targets, which make them unique in the treatment of cardiovascular disease [ 22 , 23 ]. In our previous study, we found that O. falcata extract demonstrated significant protective effects against MIRI, but the underlying mechanism is not clear.…”

Section: Discussionmentioning

confidence: 99%

7-Hydroxyflavone Alleviates Myocardial Ischemia/Reperfusion Injury in Rats by Regulating Inflammation

et al. 2022

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Inflammation is the primary pathological process of myocardial ischemia/reperfusion injury (MI/RI). 7-Hydroxyflavone (HF), a natural flavonoid with a variety of bioactivities, plays a crucial role in various biological processes. However, its cardioprotective effects and the underlying mechanisms of MI/RI have not been investigated. This study aimed to explore whether pretreatment with HF could attenuate MI/RI-induced inflammation in rats and investigate its potential mechanisms. The results showed that pretreatment with HF could significantly improve the anatomic data and electrocardiograph parameters, reduce the myocardial infarct size, decrease markers of myocardial injury (aspartate transaminase, creatine kinase, lactate dehydrogenase, and cardiac troponin I), inhibit inflammatory cytokines (IL-1β, IL-6, and TNF-α), suppress oxidative stress, and recover the architecture of the cardiomyocytes. The cardioprotective effect of HF was connected with the regulation of the MAPK/NF-κB signaling pathway. What is more, molecular docking was carried out to prove that HF could be stably combined with p38, ERK1/2, JNK, and NF-κB. In summary, this is a novel study demonstrating the cardioprotective effects of HF against MI/RI in vivo. Consequently, these results demonstrate that HF can be considered a promising potential therapy for MI/RI.

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Section: Discussionmentioning

confidence: 99%

7-Hydroxyflavone Alleviates Myocardial Ischemia/Reperfusion Injury in Rats by Regulating Inflammation

et al. 2022

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“…Interestingly, oleracein E ( 64a ) also displayed cardioprotective activity in the acute myocardial ischemic mice model (Table ) with improved cardiac function and reduction in heart inflammatory infiltration, fibrosis, and cardiomyocytes apoptosis observed. The underlying mechanism of action was associated with the inhibition of oxidative stress and regulation of the MAPK signaling pathway . Whether or not oleracein E exerts cardioprotective functions via β 2 -AR similar to higenamine needs further examination.…”

Section: Part V: Biological Activities Of Catecholamine Derivativesmentioning

confidence: 99%

“…In recent years, (+)-oleracein E ( 64a ) has also been isolated from lateral roots of Aconitum carmichaelii Debeaux (Ranunculaceae), aerial parts of Meconopsis punicea Maxim., whole plant of Meconopsis horridula Hook. f. & Thomson (Papaveraceae), Cirsium setosum (Willd.) MB.…”

Section: Part 2: Catecholamine Derivatives In Plantsmentioning

confidence: 99%

Catecholamine Derivatives: Natural Occurrence, Structural Diversity, and Biological Activity

Tang,

Zhao,

Tao

et al. 2023

J. Nat. Prod.

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Catecholamines (CAs) are aromatic amines containing a 3,4-dihydroxyphenyl nucleus and an amine side chain. Representative CAs included the endogenous neurotransmitters epinephrine, norepinephrine, and dopamine. CAs and their derivatives are good resources for the development of sympathomimetic or central nervous system drugs, while they also provide ligands important for G-protein coupled receptor (GPCR) research. CAs are of broad interest in the fields of chemical, biological, medical, and material sciences due to their high adhesive capacities, chemical reactivities, metal-chelating abilities, redox activities, excellent biocompatibilities, and ease of degradability. Herein, we summarize CAs derivatives isolated and identified from microorganisms, plants, insects, and marine invertebrates in recent decades, alongside their wide range of reported biological activities. The aim of this review is to provide an overview of the structural and biological diversities of CAs, the regularity of their natural occurrences, and insights toward future research and development pertinent to this important class of naturally occurring compounds.

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“…The mice were housed with regular food and free water in a 12 h light-dark cycles, under conditions of 22 ± 2°C. According to the previous reports, the AMI model of mice LAD ligation was used to evaluate the effect of TE, RAF and PAF [9]. The Ethics Committee of BUCM had approved the experiments (the ethics permit number: BUCM-3-2015090701-3003), and the research was implemented according to the US guidelines (NIH publication #85 − 23, revised in 1996).…”

Section: Experimental Animal and Ethics Statementmentioning

confidence: 99%

The alkaloids of Corydalis hendersonii Hemsl. contribute to the cardioprotective effect against ischemic injury in mice by attenuating cardiomyocyte apoptosis via p38 MAPK signaling pathways

Gao

zhou

et al. 2022

Preprint

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Background: There is a characteristic Tibetan folk medicine in China named Corydalis hendersoniiHemsl. (CH) has been used for treatment of cardiovascular related diseases, called “plethora” in Tibetan medicine. Previous studies demonstrated that ethanol extract of CH shows anti-acute myocardial infarction (AMI) effect through inhibiting fibrosis and inflammation. Rich alkaloids fraction (RAF) is isolated from CH, but whether RAF possessing an equivalent effect with CH ethanol extract and by which mechanism it protects against AMI has not yet reported. The paper aimed to study whether RAF protects myocardial injury in mice and its underlying mechanism. Material and methods: The cation exchange resin was used to obtain RAF and poor alkaloidal fraction (PAF). Liquid chromatography mass spectrometry-ion trap-time of flight (LCMS-IT-TOF) was used to analyze the chemical profile and isolate pure compounds. The ligation of left anterior descending (LAD) of coronary artery in mice was used to evaluate the in vivo anti-AMI effect, by dividing into eight groups: Sham, Model, Fosinopril (10 mg/kg, i.g.), total extract (TE, 400 mg/kg, i.g.), PAF (300 mg/kg, i.g.), and RAF (25, 50, and 100 mg/kg, respectively, i.g.) groups. Echocardiography was used to evaluate mice heart function through the index of left ventricular internal diameter (LVEDs), left ventricular internal diameter (LVEDd), fractional shortening (FS) and ejection fraction (EF). We detected the lactate dehydrogenase (LDH) and creatine kinase-MB (CK-MB) in the serum and the plasma level of angiotensin II (AngII). The apoptosis of mice myocardial tissue was verified by TUNEL assay. The expression of p38 mitogen-activated protein kinases (p38 MAPK), Bcl-2 and X Protein associated Bcl-2 (Bax) were detected through immunofluorescence staining, qRT-PCR and western blot in heart tissue and H9c2 cells. Results: Echocardiography data indicated that the values of LVEDd and LVEDs were reduced and the values of FS and EF were improved by TE and RAF significantly. RAF also decreased the levels of LDH, CK-MB and AngII and significantly inhibited inflammatory cells in the marginal zone of myocardial infarction. The TUNEL assay results showed that RAF significantly attenuated cell apoptosis. Immunofluorescence and qRT-PCR assay showed that RAF inhibited p38 MAPK, Bax, and Bcl-2 proteins in mice myocardium. Western blot results validated that the expressions of key proteins were inhibited by RAF. Also, the apoptotic cells and apoptosis-related proteins were dramatically reduced by RAF in vivo and in vitro. Besides, RAF and PAF were analyzed by LCMS-IT-TOF to identify the main compounds and to demonstrate the difference between them. The results showed that a total of 14 alkaloids were identified, which indicated that the isoquinoline alkaloids were the main ingredients in RAF may contributing to the cardioprotective effect in mice. Conclusions: RAF improves cardiac function by inhibiting apoptosis via p38 MAPK signaling pathways, and RAF contributed to the effect against myocardial ischemic injury of TE in mice, which provided a substantial reference for the clinical application against ischemia heart disease and quality control of CH.

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Meconopsis horridula Hook. f. & Thomson extract and its alkaloid oleracein E exert cardioprotective effects against acute myocardial ischaemic injury in mice

Cited by 20 publications

References 24 publications

7-Hydroxyflavone Alleviates Myocardial Ischemia/Reperfusion Injury in Rats by Regulating Inflammation

7-Hydroxyflavone Alleviates Myocardial Ischemia/Reperfusion Injury in Rats by Regulating Inflammation

Catecholamine Derivatives: Natural Occurrence, Structural Diversity, and Biological Activity

The alkaloids of Corydalis hendersonii Hemsl. contribute to the cardioprotective effect against ischemic injury in mice by attenuating cardiomyocyte apoptosis via p38 MAPK signaling pathways

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