2020
DOI: 10.1186/s13041-020-00695-1
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MeCP2 gates spatial learning-induced alternative splicing events in the mouse hippocampus

Abstract: Long-term memory formation is supported by functional and structural changes of neuronal networks, which rely on de novo gene transcription and protein synthesis. The modulation of the neuronal transcriptome in response to learning depends on transcriptional and post-transcriptional mechanisms. DNA methylation writers and readers regulate the activity-dependent genomic program required for memory consolidation. The most abundant DNA methylation reader, the Methyl CpG binding domain protein 2 (MeCP2), has been … Show more

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Cited by 15 publications
(10 citation statements)
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“…In the first, quantitative assessment of high-quality sequencing datasets found little variation in global AS as a result of differential MeCP2 and/or DNA methylation levels ( Chhatbar et al, 2020 ). However, in the second article, MeCP2 was found to be required for maintaining mature hippocampal AS profiles, and to regulate splicing of specific neuronal genes in the hippocampus during memory consolidation ( Brito et al, 2020 ). These two recent papers underscore the still long road ahead in understanding MeCP2’s role in AS, as well as the importance of careful context-specific interpretation of MeCP2 studies moving forward.…”
Section: Subsectionsmentioning
confidence: 99%
“…In the first, quantitative assessment of high-quality sequencing datasets found little variation in global AS as a result of differential MeCP2 and/or DNA methylation levels ( Chhatbar et al, 2020 ). However, in the second article, MeCP2 was found to be required for maintaining mature hippocampal AS profiles, and to regulate splicing of specific neuronal genes in the hippocampus during memory consolidation ( Brito et al, 2020 ). These two recent papers underscore the still long road ahead in understanding MeCP2’s role in AS, as well as the importance of careful context-specific interpretation of MeCP2 studies moving forward.…”
Section: Subsectionsmentioning
confidence: 99%
“…Relative expression levels of each target transcript were determined by the ∆∆Ct method using beta-Actin mRNA levels as a reference 35 .…”
Section: Contextual Fearmentioning
confidence: 99%
“…RNA from hippocampal cultures or tissue was extracted using the RNeasy Plus Mini Kit °C to 90 °C at a ramp rate of 0.6 °C/min. Relative expression levels of each target transcript were determined by the ∆∆Ct method using beta-Actin mRNA levels as a reference (36).…”
Section: Stereotaxic Surgerymentioning
confidence: 99%