Medaka in Japanese Agricultural Water Channels: Genetic Diversity and Conservation

Mamun, M. S. A.; Tsuchida, Koji; Onishi, Toshikazu; Hiramatsu, Ken; Iwasawa, Atsushi; Nishimura, Shinichi

doi:10.7831/ras.4.8

Cited by 2 publications

(3 citation statements)

References 59 publications

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“…However, severe threats are not restricted to medaka’s relatives from Sulawesi. Populations of both Japanese ricefish species have reportedly been declining, particularly in densely populated regions and where exotic species have been introduced (Asai et al, 2011; Mamun et al, 2016; Parenti, 2008; Parenti and Soeroto, 2004). Ricefishes were even thought to have gone extinct in Taiwan, before they were rediscovered in 1993 (Parenti, 2008; Tzeng et al, 2006).…”

Section: Threatened Diversitymentioning

confidence: 99%

“…It is often found in rice fields, which earned it its common English name of ricefish and the genus name of Oryzias (based on the genus name of rice, Oryza ). The medaka has been kept as an ornamental fish in Japan from as early as the 17 th century, and its central role in Japanese culture is exemplified by its appearance in pieces ranging from Edo era paintings (Kinoshita et al, 2009; Naruse et al, 2011; Takeda and Shimada, 2010) to modern-day children’s songs (Mamun et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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The untapped potential of medaka and its wild relatives

Hilgers

Schwarzer

2019

eLife

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The medaka is a fish that has served as a model organism for over a century, yet there is still much to learn about its life in the wild. Here we summarize the current knowledge, highlight recent progress and outline remaining gaps in our understanding of the natural history of medaka. It has also become clear over time that rather than being a single species, medaka comprises an entire species complex, so disentangling the species boundaries is an important goal for future research. Moreover, medaka and other ricefishes exhibit striking functional diversity, little of which has been investigated to date. As such, there are opportunities to use the resources developed for medaka to study other ricefishes, and to learn more about medaka itself in an evolutionary context.

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Section: Threatened Diversitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The untapped potential of medaka and its wild relatives

Hilgers

Schwarzer

2019

eLife

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“…In st. 5, lining of channels was changed to three-sides concrete after the previous capture survey (2010). This human activity might cause habitat manipulation because the medaka species are vulnerable to anthropogenic habitat disturbances 31 , 32 . Our eDNA analysis with high detection capability can be an effective tool for tracing a species habitat changes.…”

Section: Discussionmentioning

confidence: 99%

Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species

Tsuji

Iguchi

Shibata

et al. 2018

Sci Rep

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Information about species distribution is crucial to ecological studies. Environmental DNA (eDNA) analysis has recently been used to estimate the distribution of aquatic organisms. Several analytical methods including metabarcoding and species-specific PCR are being used for eDNA analysis. However, when only a few species are targeted, metabarcoding is not cost-effective because of the wasted consumption of read due to amplification of non-target species DNA. On the other hand, species-specific PCR requires tests to be repeated multiple times resulting in consuming more DNA templates, and experimental consumables. Here we propose a methodological framework for simultaneously detecting a few species using real-time multiplex PCR. We developed the species-specific primer-probe sets for two species of Japanese medaka (Oryzias latipes and o. sakaizumii), and we used them in the real-time multiplex PCR. In aquarium experiment, even when the species abundances were biased, both species were simultaneously detected in all samples. In a field survey, eDNA analysis and capture survey produced consistent results in all sampling sites, including sites with low fish densities. eDNA analysis using real-time multiplex PCR can be easily applied to other aquatic organisms, enabling a more cost-effective distribution survey of multiple target organisms.

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Medaka in Japanese Agricultural Water Channels: Genetic Diversity and Conservation

Cited by 2 publications

References 59 publications

The untapped potential of medaka and its wild relatives

The untapped potential of medaka and its wild relatives

Real-time multiplex PCR for simultaneous detection of multiple species from environmental DNA: an application on two Japanese medaka species

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