Media and Dilution Procedures Tested to Minimize Handling Effects on Human, Rabbit, and Bull Sperm for Computer‐Assisted Sperm Analysis (CASA)

FARRELL, PAUL B.; FOOTE, ROBERT H.; McARDLE, MARGARET M.; TROUERN‐TREND, VICTORIA L.; TARDIF, ANGELA L.

doi:10.1002/j.1939-4640.1996.tb01785.x

Cited by 48 publications

(3 citation statements)

References 34 publications

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“…CASA can significantly improve the accuracy and precision of dog semen analysis methods. Furthermore, CASA has been successfully used in other mammalian species, such as bulls, boars, and humans, to improve IVF outcomes ( 28 , 29 ).…”

Section: Discussionmentioning

confidence: 99%

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Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene

Hossein,

Son,

Jeong

et al. 2023

Front. Vet. Sci.

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Propagation of transgenic animals by germline transmission using assisted reproductive technologies such as in vitro fertilization (IVF) is the most efficient way to produce transgenic colonies for biomedical research. The objective of this study was to generate transgenic puppies from a founder dog expressing the mutated human amyloid precursor protein (mhAPP) gene. Experiment I assessed the characteristics of the semen prepared by freshly diluted, swim-up, and Percoll gradient methods using a computer-assisted semen analyzer (CASA). Motile and progressively motile sperm counts were higher in the Percoll gradient samples (p < 0.05) than in the swim-up and freshly diluted samples. In Experiment II, a total of 59, 70, and 65 presumptive zygotes produced by fresh, Percoll gradient, and swim-up methods, respectively, were transferred to surrogates (5 for each group); the Percoll gradient (27.27%) and swim-up samples (14.29%) showed the highest blastocyst formation rates, while fresh diluted semen did not produce any blastocyst. Experiment III examined the full-term developmental ability of embryos. Among the 5 surrogates in the Percoll gradient group, one (20.0%) became pregnant; it had 4 (6.15%) sacs and delivered 4 (6.15%; 2 males and 2 females) live puppies. Among the 4 puppies, 2 (50.0%) were found to transmit the transgene on their nail and toe under GFP fluorescence. Furthermore, the integration and expression of the mhAPP transgene were examined in the umbilical cords of all the IVF-derived puppies, and the presence of the transgene was only observed in the GFP-positive puppies. Thus, semen prepared by the Percoll method could generate transgenic puppies by male germline transmission using the IVF technique. Our result will help propagate transgenic dogs efficiently, which will foster human biomedical research.

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Section: Discussionmentioning

confidence: 99%

“…Several reports describing mammalian sperm preparation using swim-up and Percoll gradient methods have been published ( 28 , 29 ). However, these reports are inconsistent.…”

Section: Discussionmentioning

confidence: 99%

Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene

Hossein,

Son,

Jeong

et al. 2023

Front. Vet. Sci.

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“…Furthermore, we have shown that pure chitosan microcapsules did not aggregate with sperm either, did not affect cell viability and motility parameters, and presumably did not interfere with the AI system used in vivo. As the commercial diluent used is designed for use at RT instead of 37•C, it is possible that the pronounced decrease in sperm motility over time can be attributed to the temperature and the time incubation applied during the experiment [44,45].…”

Section: Discussionmentioning

confidence: 99%

Microencapsulated rrbNGF as an Alternative Ovulation Induction Method in Rabbits

Quiroga

Gimeno-Martos

Lorenzo

et al. 2023

Preprint

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Background Rabbits are a reflexive ovulatory species, and intramuscular administration of exogenous hormonal factors such as gonadotropin-releasing hormone (GnRH) analogues at artificial insemination (AI) are traditionally used to induce ovulation. To find more animal-friendly ovulation methods, the addition of a molecule naturally present in the seminal plasma, such as nerve growth factor (NGF) microencapsulated with chitosan for protection and administered by intravaginal via, has been studied. First, its biological activity in pheochromocytoma of the rat adrenal medulla (PC12) cell cultures and its effects in semen were tested. Subsequently, the ability of the intravaginal NGF-chitosan delivery system administered at AI time (NGFch-0) or 30 min before AI (NGFch-30) in eliciting ovulation estimating progesterone concentrations on Day 7 post AI, as well as fertility and prolificacy results in nulliparous and multiparous rabbit does, were compared with the treatment with an analogue of GnRH (GnRH), as well as with the insertion of an empty (C-e) or containing diluted semen (C-s) catheter. Results NGF-chitosan could promote PC12 differentiation to the same extent as free NGF without impairing cell viability. Microcapsules did not interfere with semen motility, viability or capacitation status. All groups of nulliparous rabbit does responded to all treatments reaching ovulation rates statistically similar between them (GnRH 90%; NGFch-30 100%; NGFch-0 66.7% and C-e 83.3%), with higher fertility rates in GnRH and NGFch-30 groups (90 and 100%, respectively) than in NGFch-0 (60%) and similar prolificacy results. However, multiparous females showed reduced ovulation and fertility rates upon NGF-chitosan exposure regardless of administration time or after the mechanical stimulus compared to the GnRH group. In both C-e and C-s groups was also observed a significant ovulatory response. Conclusions We have demonstrated that NGF-chitosan administration 30 min before AI-induced ovulation at a similar rate to GnRH injection in nulliparous but not in multiparous does. A better receptivity status of nulliparous females could be a determining factor of this response. Nonetheless, mechanical stimulation also determined a high ovulation induction rate, thus masking the NGF-derived effect.

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Computerized Imaging and Scanning Electron Microscope (SEM) Analysis of Co‐Cultured Fresh and Frozen Bovine Sperm

SUZUKI,

FOOTE,

FARRELL

1997

Journal of Andrology

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Fresh and frozen‐thawed bull sperm were incubated with bovine oviductal epithelial cells and segments from the oviducts to examine the usefulness of these culture systems to model sperm changes in vivo. Changes in sperm motion characteristics [computer‐assisted sperm analysis (CASA)] and surface morphology [scanning electron microscopy (SEM)] were evaluated. In Experiment 1, fresh and frozen sperm were suspended in the Brackett and Oliphant medium or modified Tyrodes medium (mTALP) and incubated for 0, 3, 6, and 9 hours in direct contact with bovine oviductal‐epithelial cell (BOEC) monolayers prepared from oviducts of cows in the periovulatory phase of estrus. The percentage of motile sperm decreased gradually in mTALP, but decreased rapidly in Brackett's defined medium after 3 hours of incubation, with overall averages of 55 and 32%, respectively. The percentage of motile fresh sperm exceeded frozen‐thawed sperm under all conditions. In Experiment 2, sperm suspended with mTALP were incubated in dishes without monolayers (control), with monolayers, and within the segments of the oviduct for 0, 3, and 6 hours. In the epithelial cell monolayers, the percentage of motile sperm was similar to the controls throughout incubation, but after 3 hours in the oviductal segments, a decrease, partly associated with more rapid rupture of acrosomal membranes occurred. Sperm velocity was higher (100 μm/seconds) in fresh sperm than in frozen sperm (85 μm/seconds). Acrosomal changes, discernible with SEM after 3 hours of incubation, increased with time and were always found more often in frozen than in fresh sperm. The BOEC‐monolayer system provided a useful in vitro model to study pre‐fertilization changes in sperm.

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Media and Dilution Procedures Tested to Minimize Handling Effects on Human, Rabbit, and Bull Sperm for Computer‐Assisted Sperm Analysis (CASA)

Cited by 48 publications

References 34 publications

Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene

Production of transgenic first filial puppies expressing mutated human amyloid precursor protein gene

Microencapsulated rrbNGF as an Alternative Ovulation Induction Method in Rabbits

Computerized Imaging and Scanning Electron Microscope (SEM) Analysis of Co‐Cultured Fresh and Frozen Bovine Sperm

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