Media and methods for separation and cultivation of oral spirochetes

Rosebury, Theodor; Macdonald, James B.; Ellison, Solon A.; Engel, Sylvia G.

doi:10.1016/0030-4220(51)90525-7

Cited by 15 publications

(4 citation statements)

References 22 publications

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“…Oral sl)ilochetes are usually cultivated on a coml)lex Tyndallized veal heart infusion medium containing ascitic fluid (Hampp, 1943;Rosebury et al, 1951) or rabbit blood (B3erger, 1958; Socranskv, Macdonald, and Sawyer, 1959). Attempts to simplify and define this medium have met with partial success.…”

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confidence: 99%

DEPENDENCY OFTREPONEMA MICRODENTIUMON OTHER ORAL ORGANISMS FOR ISOBUTYRATE, POLYAMINES, AND A CONTROLLED OXIDATION-REDUCTION POTENTIAL

Socransky¹,

Loesche²,

Hubersak³

et al. 1964

J Bacteriol

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confidence: 99%

DEPENDENCY OFTREPONEMA MICRODENTIUMON OTHER ORAL ORGANISMS FOR ISOBUTYRATE, POLYAMINES, AND A CONTROLLED OXIDATION-REDUCTION POTENTIAL

Socransky¹,

Loesche²,

Hubersak³

et al. 1964

J Bacteriol

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“…Dr. Ellison got his PhD at Columbia, first working with Rosebury and then with Dr. Harry M. Rose (Rosebury et al, 1951)…”

Section: The Ellison Linementioning

confidence: 99%

Dr. Theodor Rosebury: Grandfather of Modern Oral Microbiology

Fine

2006

J Dent Res

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the 1940s. In 1971, the IADR meeting was in Chicago. After hearing the spirochete story, and realizing that we would be in Chicago and that Rosebury was living in Chicago, Irwin decided to arrange for a visit to Rosebury's home. The visit was to include my wife, Dr. Solon A. Ellison and Dr. Mandel (both of whom had been Rosebury's students at Columbia Dental School), and me. At that time, Dr. Ellison was the director and Chair of the Oral Biology Department at SUNY Buffalo (Emmings, 1999). I had heard that, since his retirement, Rosebury had dedicated himself to writing, cabinetmaking, and music. I had heard that he excelled in each area, and had become a consummate cabinetmaker, a renowned writer, and an exceptionally gifted flutist. It was with great anticipation that I arrived at the house of this revered microbiologist. Rosebury greeted his students, Mandel and Ellison, warmly. Dr. Mandel introduced my wife and me to Dr. Rosebury and reported that Rosebury had known several members of my wife's family and that her uncle Sam had worked in his laboratory. Rosebury expressed fond memories of Sam and the research they had done together and invited us to come up to his attic, where he had his reference library. It was truly remarkable to see the extent of his reference card catalogue, considering that he had retired from academics several years before. The card catalogue consisted of thousands of meticulously labeled 3 x 5 index cards in a space equivalent to a small university library. He then located a card that catalogued an article he had published with my wife's uncle in the Journal of Dental Research, entitled "Studies of lactobacilli in relation to caries in rats. II. Attempt to immunize rats on caries producing diets against lactobacilli" (Rosebury et al., 1934). I later discovered that caries vaccine scientists refer to this article as one of the first papers that attempted to document a vaccine developed to a caries-producing organism. WHO WAS THEODOR ROSEBURY?Ted Rosebury was born in London on August 10, 1904. His family moved to the United States when he was six, and he became a naturalized citizen when he was 12 years old. He attended the University of Pennsylvania, where he received his DDS in 1928. In his own words, while in his junior year of dental school, he and a fellow dental student "read and studied (Bulletin Number Nineteen -A survey of dental education in the United States and Canada -conducted by Dr. William J.

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“…The methods for the isolation of the Treponemataceae are modifications of the principle advocated by Noguchi (1912a, b), which depended upon the ability of the spiral organisms to migrate away from the contamination in soft agar or gel. More recently, Rosebury et al (1951) proposed a modification of the Noguchi method; they used small petri plates as containers for primary cul-tures with the wall of a central well in an agar plate as the site of inoculation. This method eliminated the effects of disruption of the agar by the gas-producing bacteria and greatly improved the isolation procedures.…”

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confidence: 99%