Media Characterization under Scattering Conditions by Nanophotonics Iterative Multiplane Spectroscopy Measurements

Abstract

Characterizing materials is preferably done by multiple wavelengths. In opaque materials, the scattering poses a challenge due to the additional complexity to the spectroscopic measurements. We have previously demonstrated an iterative multiplane method for characterizing materials using the reflection from turbid media. Initial studies were performed in the red wavelength regime (632.8 nm) which is optimal for biomedical applications. However, in order to differentiate between materials, it is better to use multiple wavelengths, as spectroscopy may detect the material fingerprint. In this paper, our iterative multiplane optical property extraction (IMOPE) technique is presented in the blue regime (473 nm). Agar-based solid phantom measurements were conducted and compared to our theoretical model. Compatibility between experiments in the red and blue wavelengths shows the robustness of our technique.

Depth Scattering Characterization of Multi-Layer Turbid Media Based on Iterative Multi-Plane Reflectance Measurements

“…Those two regimes are known to have opposite behavior, hence the phase RMS is calculated from each regime separately. Initially [22,23,25], the border between those two regimes was chosen manually and found to be correlated with the transport Mean Free Path (MFP'=1/s'). In this paper, we present a methodology for extracting this transition point automatically.…”

“…Mostly, the IMOPE was applied for biomedical purposes hence the red wavelength was used. However, recently, we also showed the feasibility of the IMOPE as a spectral tool [25], using a blue wavelength as well as the initial red one.…”

“…We have presented previously the iterative multi-plane optical property extraction (IMOPE) technique [19][20][21][22][23][24][25]. The IMOPE technique is a noninvasive nanophotonics technique for extracting the scattering properties of a medium based on a single light source.…”

“…First, single layer tissue-like phantoms were measured by the IMOPE, for calibration purposes. As was reported before [22][23][24][25], two distinguishable regions were visible in the phase images and were marked manually as two spatial regions that correlated to two scattering regimes. Here, a methodology for finding these two regions automatically based on RMS computations from the phase images is presented.…”

Noninvasive Nanodiamond Skin Permeation Profiling Using a Phase Analysis Method: Ex Vivo Experiments

“…For more information regarding the phase assessment and analysis, see the Supporting Information, section 1. The IMOPE technique was first designed for the red wavelength (633 nm) and was later extended to the blue regime (473 nm that is used in this research) . In literature, porcine skin is considered to resemble human skin in various characteristics, such as structure, thickness and optical properties. − Therefore, we used a porcine skin tissue for simulating the skin-penetration abilities of the fNDs.…”