Media Standardization for Hydroponic Culture to Screen  Wheat Genotypes for Nitrogen Use Efficiency

Ranjitha, K.; Biradar, Suma; Desai, S; Naik, V. Rudra; Bhat, Sumangala; Satisha, T.N.; Hiremath, G. M.; Kumar, Khirod; Chethana, C.K.; Venkatesh, Karnam

doi:10.20546/ijcmas.2017.612.327

Cited by 3 publications

(8 citation statements)

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“…We conducted a hydroponic screen of 282 barley accessions to observe phenotypic changes under low-N treatment. Several studies have been carried out so far in wheat, maize, and barley using hydroponics as a screening method ( Sun et al, 2013 ; Yang et al, 2014 ; Liu et al, 2017 ; Ranjitha et al, 2017 ; Xiong et al, 2019 ) because of the ability to supply precise amounts of nutrients and easy observation of root characteristics. Furthermore, it has been reported that the root system architecture-related traits studied in hydroponic experiments have a positive correlation with nutrient (mainly N and P) uptake efficiency traits ( Liu et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Hordeum vulgare L.)

et al. 2020

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Nitrogen (N) fertilizer is largely responsible for barley grain yield potential and quality, yet excessive application leads to environmental pollution and high production costs. Therefore, efficient use of N is fundamental for sustainable agriculture. In the present study, we investigated the performance of 282 barley accessions through hydroponic screening using optimal and low NH 4 NO 3 treatments. Low-N treatment led to an average shoot dry weight reduction of 50%, but there were significant genotypic differences among the accessions. Approximately 20% of the genotypes showed high (>75%) relative shoot dry weight under low-N treatment and were classified as low-N tolerant, whereas 20% were low-N sensitive (≤55%). Low-N tolerant accessions exhibited well-developed root systems with an average increase of 60% in relative root dry weight to facilitate more N absorption. A genome-wide association study (GWAS) identified 66 significant marker trait associations (MTAs) conferring high nitrogen use efficiency, four of which were stable across experiments. These four MTAs were located on chromosomes 1H(1), 3H(1), and 7H(2) and were associated with relative shoot length, relative shoot and root dry weight. Genes corresponding to the significant MTAs were retrieved as candidate genes, including members of the asparagine synthetase gene family, several transcription factor families, protein kinases, and nitrate transporters. Most importantly, the highaffinity nitrate transporter 2.7 (HvNRT2.7) was identified as a promising candidate on 7H for root and shoot dry weight. The identified candidate genes provide new insights into our understanding of the molecular mechanisms driving nitrogen use efficiency in barley and represent potential targets for genetic improvement.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Hordeum vulgare L.)

et al. 2020

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“…In fields, N availability should be measured at multiple sites rather than merging a common value for the whole field because N in the soil can vary over very short distances. Therefore, pot and hydroponic experiments in growth chambers have been extensively conducted [12,58]. A comparison of all three screening methods revealed that the latter two approaches reduce environmental interference on genetic screening [29].…”

Section: Nue Screening and Phenotypingmentioning

confidence: 99%

“…However, many studies have suggested that hydroponic experiments overcome the technical difficulties in root phenotyping in N uptake researches [12,62]. Hydroponics, using a nutrient solution as the cultivation medium instead of soil, facilitates the study of the N uptake mechanism and its impact on plant growth [63] with its easy observation of both root and shoot characteristics.…”

Section: Nue Screening and Phenotypingmentioning

confidence: 99%

“…Hydroponics, using a nutrient solution as the cultivation medium instead of soil, facilitates the study of the N uptake mechanism and its impact on plant growth [63] with its easy observation of both root and shoot characteristics. Recently, a hydroponic experiment examined the shoot and root traits of five wheat genotypes at four different levels of N to identify high NUE genotypes [12]. Likewise, a hydroponic experiment on 82 Tibetan barley accessions investigated their performance under low N in terms of shoot and root dry biomass [64].…”

Section: Nue Screening and Phenotypingmentioning

confidence: 99%

“…Several studies have been undertaken to improve NUE in crops including rice, wheat and maize [8][9][10][11]. Starting from simple phenotypic screening through to advanced molecular techniques, crop performance under low N has been improved [12,13]. There are a few success stories for rice NUE improvement by genetic engineering [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Advances in Understanding the Molecular Mechanisms and Potential Genetic Improvement for Nitrogen Use Efficiency in Barley

et al. 2020

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Nitrogen (N) fertilization plays an important role in crop production; however, excessive and inefficient use of N fertilizer is a global issue that incurs high production costs, pollutes the environment and increases the emission of greenhouse gases. To overcome these negative consequences, improving nitrogen use efficiency (NUE) would be a key factor for profitable crop production either by increasing yield or reducing fertilizer cost. In contrast to soil and crop management practices, understanding the molecular mechanisms in NUE and developing new varieties with improved NUE is more environmentally and economically friendly. In this review, we highlight the recent progress in understanding and improving nitrogen use efficiency in barley, with perspectives on the impact of N on plant morphology and agronomic performance, NUE and its components such as N uptake and utilization, QTLs and candidate genes controlling NUE, and new strategies for NUE improvement.

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Efficiency of Treated Domestic Wastewater to Irrigate Two Rice Cultivars, PK 386 and Basmati 515, under a Hydroponic Culture System

Aslam,

Mirza,

Rashid

et al. 2023

Water

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The increasing human population continues to exert pressure on the freshwater scarcity. The availability of freshwater for crop irrigation has become challenging. The present study aimed to use domestic wastewater (DWW) for the irrigation of two rice cultivars (CVs) after treatment with the bacterial strain Alcaligenes faecalis MT477813 under a hydroponic culture system. The first part of this study focused on the bioremediation and analysis of the physicochemical parameters of DWW to compare pollutants before and after treatment. The biotreatment of DWW with the bacterial isolate showed more than 90% decolourisation, along with a reduction in contaminants. The next part of the study evaluated the impacts of treated and untreated DWW on the growth of two rice cultivars, i.e., PK 386 and Basmati 515, under a hydroponic culture system which provided nutrients and water to plants with equal and higher yields compared to soil. Growth parameters such as the shoot and root length and the wet and dry weights of the rice plants grown in the treated DWW were considerably higher than those for the plants grown in untreated DWW. Therefore, enhanced growth of both rice cultivars grown in biotreated DWW was observed. These results demonstrate the bioremediation efficiency of the bacterial isolate and the utility of the DWW for rice crop irrigation subsequent to biotreatment.

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Media Standardization for Hydroponic Culture to Screen Wheat Genotypes for Nitrogen Use Efficiency

Cited by 3 publications

References 4 publications

Genome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Hordeum vulgare L.)

Genome-Wide Association Study and Identification of Candidate Genes for Nitrogen Use Efficiency in Barley (Hordeum vulgare L.)

Advances in Understanding the Molecular Mechanisms and Potential Genetic Improvement for Nitrogen Use Efficiency in Barley

Efficiency of Treated Domestic Wastewater to Irrigate Two Rice Cultivars, PK 386 and Basmati 515, under a Hydroponic Culture System

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