Mediation of JA signalling in glandular trichomes by the <i>woolly/SlMYC1</i> regulatory module improves pest resistance in tomato

Hua, Bing; Chang, Jiang; Wu, Minliang; Xu, Zhijing; Zhang, Fanyu; Yang, Meng‐Yin; Xu, Huimin; Chen, Xiao‐Ya; Wu, Shuang

doi:10.1111/pbi.13473

Cited by 70 publications

(48 citation statements)

References 65 publications

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“…SlMYC1 function seems to be related to the expansion of stalk cells and glandular cells in type VI trichomes (Xu et al ., 2018). Intriguingly, SlMYC1 also interacts with SlJAZ4 , implying that SlMYC1 could also be involved in JA‐induced trichome morphogenesis (Hua et al ., 2020). Consistent with tomato, the expression of GhMYC2 , a close homologue of SlMYC1 in cotton, is reduced in RNA interference‐GhHOX3 lines (Shan et al ., 2014).…”

Section: Discussionmentioning

confidence: 99%

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

Hua

Chang

et al. 2021

New Phytologist

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Plant hormones can adjust the physiology and development of plants to enhance their adaptation to biotic and abiotic challenges. Jasmonic acid (JA), one of the immunity hormones in plants, triggers genome-wide transcriptional changes in response to insect attack and wounding. Although JA is known to affect the development of trichomes, epidermal appendages that form a protective barrier against various stresses, it remains unclear how JA interacts with developmental programs that regulate trichome development. In this study, we show that JA affects trichome length in tomato by releasing the transcriptional repression mediated by Jasmonate ZIM (JAZ) proteins. We identified SlJAZ4, a negative regulator preferentially expressed in trichomes, as the critical component in JA signaling in tomato trichomes. We also identified a homeodomain-leucine zipper gene, SlHD8, as the downstream regulator of JA signaling that promotes trichome elongation. SlHD8 is also highly expressed in trichomes and physically interacts with SlJAZ4. Loss-of-function mutations in SlHD8 caused shorter trichomes, a phenotype that was only partially rescued by methyl jasmonate treatment. Our dual-luciferase and chromatin immunoprecipitation-quantitative PCR assays revealed that SlHD8 regulates trichome elongation by directly binding to the promoters of a set of cellwall-loosening protein genes and activating their transcription. Together, our findings define SlHD8-SlJAZ4 as a key module mediating JA-induced trichome elongation in tomato.

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Section: Discussionmentioning

confidence: 99%

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

Hua

Chang

et al. 2021

New Phytologist

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“…Jasmonic acid is a universal phytohormone that regulates trichome development; for example, JA induces trichome initiation in Arabidopsis, A. annua , and tomato and trichome elongation in cotton ( Traw and Bergelson, 2003 ; Hu et al, 2016 ; Yan et al, 2017 , 2018 ; Hua et al, 2021a , b ). JA-deficient plants may have reduced numbers of trichomes ( Yan et al, 2013 ); the JA-isoleucine (JA-Ile) receptor may be involved in trichome development ( Li et al, 2004 ).…”

Section: Phytohormones Are Important Regulators Of Trichome Developmentmentioning

confidence: 99%

“…SA can interact with JA to exert various functions ( Liu et al, 2016 ). JA is a positive regulator of trichome size and trichome density ( Maes et al, 2011 ; Hua et al, 2021a , b ), and SA can inhibit JA signaling by degrading ORA59 in Arabidopsis. In trichome development, SA negatively regulates trichome density and constitutively weakens the effect of JA, indicating that crosstalk occurs between the SA- and JA-dependent trichome development pathways ( Traw and Bergelson, 2003 ).…”

Section: Phytohormones Are Important Regulators Of Trichome Developmentmentioning

confidence: 99%

Phytohormone-Based Regulation of Trichome Development

Wang

Jiang

et al. 2021

Front. Plant Sci.

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Phytohormones affect plant growth and development. Many phytohormones are involved in the initiation of trichome development, which can help prevent damage from UV radiation and insect bites and produce fragrance, flavors, and compounds used as pharmaceuticals. Phytohormones promote the participation of transcription factors in the initiation of trichome development; for example, the transcription factors HDZIP, bHLH and MYB interact and form transcriptional complexes to regulate trichome development. Jasmonic acid (JA) mediates the progression of the endoreduplication cycle to increase the number of multicellular trichomes or trichome size. Moreover, there is crosstalk between phytohormones, and some phytohormones interact with each other to affect trichome development. Several new techniques, such as the CRISPR-Cas9 system and single-cell transcriptomics, are available for investigating gene function, determining the trajectory of individual trichome cells and elucidating the regulatory network underlying trichome cell lineages. This review discusses recent advances in the modulation of trichome development by phytohormones, emphasizes the differences and similarities between phytohormones initially present in trichomes and provides suggestions for future research.

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“…MeJA is the plant hormone that plays important roles in secondary metabolite production and glandular trichome development Boughton et al, 2005;Wang et al, 2010;Maes et al, 2011;Montiel et al, 2011;Yu et al, 2012;Shen et al, 2016;Yan et al, 2017;Hua et al, 2021). However, there is no publication regarding the effect of MeJA on cannabinoid production.…”

Section: Meja Regulates Cannabinoid Biosynthesismentioning

confidence: 99%

“…It is demonstrated in Artemisia and tomato that MeJA upregulated TFs that positively regulate glandular trichome formation and increased artemisinin content in Artemisia and terpene production in tomato (Yan et al, 2017;Chalvin et al, 2020;Schuurink & Tissier, 2020). Furthermore, JA responsive TFs have been characterized in various plant species that can directly or indirectly regulate transcription of terpene biosynthetic genes (Van Der Fits & Memelink, 2000;Li et al, 2015;Jiang et al, 2016;Shen et al, 2016;Nolan et al, 2017;Chuang et al, 2018a,b;Hua et al, 2021). The alteration of these MeJA upregulated TFs resulted in changes in secondary metabolite levels.…”

Section: Studies Have Shown That Meja Controls Secondary Metabolite Biosynthesis By Regulatingmentioning

confidence: 99%

Delineating the genetic regulation of cannabinoid biosynthesis during female flower development in Cannabis sativa

Apicella

Sands

et al. 2021

Preprint

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SUMMARYCannabinoids are predominantly produced in the glandular trichomes on cannabis female flowers. There is little known on how cannabinoid biosynthesis is regulated during female flower development. We aim to understand the rate-limiting step(s) in the cannabinoid biosynthetic pathway.We investigated the transcript levels of cannabinoid biosynthetic genes as well as cannabinoid contents during 7 weeks of female flower development. We demonstrated that the enzymatic steps for producing CBG, which involve genes GPPS, PT and OAC, could be rate limit cannabinoid biosynthesis. Our findings further suggest that cannabinoid synthases, CBDAS and THCAS in a hemp and medical marijuana variety respectively, are not critical for cannabinoid biosynthesis. The cannabinoid biosynthetic genes are generally upregulated during flower maturation, which indicate glandular trichome development.MeJA can potentially increase cannabinoid production. We propose that biweekly application of 100 μM MeJA staring from flower initiation would be efficacious for promoting cannabinoid biosynthesis.Our findings suggest that the step of CBG production could rate limit the terminal cannabinoid biosynthesis. In addition, different cannabis varieties demonstrated discrete transcriptional regulation of cannabinoid biosynthetic genes.

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Mediation of JA signalling in glandular trichomes by the woolly/SlMYC1 regulatory module improves pest resistance in tomato

Cited by 70 publications

References 65 publications

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

HOMEODOMAIN PROTEIN8 mediates jasmonate‐triggered trichome elongation in tomato

Phytohormone-Based Regulation of Trichome Development

Delineating the genetic regulation of cannabinoid biosynthesis during female flower development in Cannabis sativa

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