Mediator of RNA polymerase II transcription subunit 19 promotes osteosarcoma growth and metastasis and associates with prognosis

Yu, Wenbin; Zhang, Zhichang; Min, Daliu; Yang, Qingcheng; Du, Xue-Fei; Tang, Lina; Lin, Feng; Sun, Yong; Zhao, Hui; Zheng, Shuier; He, Aina; Li, Hongtao; Yao, Yang; Shen, Zan

doi:10.1016/j.ejca.2014.01.030

Cited by 21 publications

(17 citation statements)

References 26 publications

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“…Conventional factors, such as pathological clinical stages and metastasis, are important predictors of poor clinical outcome of osteosarcoma in clinical practice. Furthermore, a number of less common molecular markers have also shown prognostic roles, such as Mediator of RNA polymerase II transcription subunit 19 (Med19), microRNA-9 (miR-9), and human leukocyte antigen (HLA) class I antigen expressions [25][26][27]. However, these markers are only available following surgery or their measurement is complex, and therefore, they are often not used in clinical practice.…”

Section: Discussionmentioning

confidence: 98%

Serum C-reactive protein and overall survival of patients with osteosarcoma

Tian

Wang

et al. 2015

Tumor Biol.

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Increased level of serum C-reactive protein (CRP) has been identified as an important prognostic factor in several types of cancers. However, the prognostic significance of serum CRP levels in patients with osteosarcoma was still unclear. A retrospective cohort study of 85 patients was performed to assess the prognostic significance of serum CRP level in osteosarcoma. Both log-rank test and multivariable analysis by Cox regression model were used to assess the impact of serum CRP levels on the overall survival in patients with osteosarcoma. Among those 85 patients, 28 (32.9 %) had high serum CRP level (>1 mg/dL), while the other 57 (67.1 %) patients had normal serum CRP level (≤ 1 mg/dL). There was no obvious difference in the baseline characteristics between high CRP group and normal CRP group. Kaplan-Meier product-limit method showed that patients with high serum CRP levels had significantly poorer overall survival than those patients with normal serum CRP levels (log-rank test P = 0.0008). Multivariable analysis by Cox regression model further showed that high serum CRP level was an independent predictor of poor overall survival (hazard ratio [HR] = 2.39; 95 % confidence interval [95 % CI] 1.22-4.67, P = 0.01). Thus, serum CRP level has an important prognostic significance in patients with osteosarcoma, and high CRP level is associated with worse overall survival.

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Section: Discussionmentioning

confidence: 98%

Serum C-reactive protein and overall survival of patients with osteosarcoma

Tian

Wang

et al. 2015

Tumor Biol.

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“…In parallel, immunohistochemical staining was performed adjacent sections using a mouse anti-Cyclin D1 antibody (1:80; DAKO, Glostrup, Denmark) to evaluate tumor proliferation [38]. Terminal deoxynucleotide transferase (TdT)–mediated dUTP-biotin nick-end labeling (TUNEL) assay was conducted to detect apoptotic cells in the tumor tissues according to manufacturer's instructions (Promega, Madison, WI, USA) [39].…”

Section: Methodsmentioning

confidence: 99%

Lumbar puncture-administered resveratrol inhibits STAT3 activation, enhancing autophagy and apoptosis in orthotopic rat glioblastomas

et al. 2016

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Trans-resveratrol suppresses glioblastoma growth in vitro, but its effects on intracranial glioblastomas remain untested. Resveratrol crosses the blood–brain barrier, and lumbar puncture (LP) greatly increases its bioavailability in rat brains; therefore, we investigated the effectiveness of LP-administered resveratrol on orthotopic rat glioblastomas. Twenty-four tumor-bearing rats were separated into two groups: Group 1 receiving 100 μl saline containing 0.3% DMSO and Group 2 receiving 100 μl resveratrol (300 μM). Treatments started 3 days after transplantation in 2-day intervals until death. Intracranial drug availabilities, tumor sizes, average life spans and the impacts on STAT3 signaling, apoptosis and autophagy rates were evaluated. MRI imaging revealed that average tumor size in the LP group (495.8 ± 22.3 mm2) was smaller than the control groups (810.3 ± 56.4 mm2; P<0.05). The mean survival time in the LP group (22.2 ± 2.1 d) was longer than control animals (16.0 ± 1.8 d; P<0.05). LP resveratrol-treated glioblastomas showed less Cyclin D1 staining, enhanced autophagy with up-regulated LC3 and Beclin1 expression, and widely distributed apoptotic foci around tumor capillaries with suppressed STAT3 expression and nuclear translocation. In conclusion, LP-delivered resveratrol efficiently inhibited orthotopic rat glioblastoma growth by inactivating STAT3 signaling and enhancing autophagy and apoptosis.

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“…In human prostate cancer cells PC-3 and DU145, knockdown of Med19 induced apoptosis via modulation of Bid and Caspase 7 (Cui et al, 2011c). Recent evidence has shown that Med19 knockdown can induce apoptosis in osteosarcoma celll line Saos-2 via induction of caspase-3 and poly ADP-ribose polymerase (PARP), along with the downregulated expression of cyclin D1 and cyclin B1 (Yu et al, 2014). Inhibition of cyclin D1 enhanced cisplatin sensitivity have been documented in human breast cancer MCF-7 and human oral squamous cell carcinoma cells.…”

Section: 875 Knockdown Of Med19 Enhances Chemo-sensitivity To Cisplamentioning

confidence: 99%

“…SiRNA targeting the sequence of human Med19 gene (NM_153450.1) is 5'-GGTGAAGGAGAAGCTAAGT-3', designed with an online software of Invitrogen and has been evidenced to be effective in many reports (Cui et al, 2011b;Li et al, 2011b;Zou et al, 2011b;Li et al, 2011b;Sun et al, 2011b;Wang et al, 2011b;Zhang et al, 2012b;Ji-Fu et al, 2012b;Yu et al, 2014). The sequences for the control siRNA were sense: 5'-UUCUCCGAACGUGUCACGUTT-3'; antisense: 5'-ACGUGACACGUUCGGAGAATT-3'.…”

Section: Med19 Sirna Transfectionmentioning

confidence: 99%