Mediator Release after Nasal Airway Challenge with Allergen

Naclerio, Robert M.; Creticos, Peter S.; Norman, Philip S.; Lichtenstein, Lawrence M.

doi:10.1164/arrd.1986.134.5.1102

Cited by 373 publications

(383 citation statements)

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“…To prevent nasal congestion, 0.25 ml oxymetazoline (0.1%) was sprayed into each nostril 5 min before the first challenge (prescribed by the Hospital Ethical Committee). Nasal lavage was performed as described before (Garrelds et al, 1994;Gerth van Wijk et al, 1992;Naclerio et al, 1983). Both nostrils were washed with 5 ml saline, prewarmed to 37°C.…”

Section: Nasal Challenge and Lavagementioning

confidence: 99%

“…Human allergen-induced responses in the nose are used as a model for allergic inflammation (Gerth van Wijk et al, 1992;Naclerio et al, 1983). After provocation with antigen, mediators, such as prostaglandin D 2, leukotriene E4, tryptase and histamine, are released during the so-called early allergic reaction, which causes sneezing, nasal blockage and rhinorrhoea.…”

Section: Introductionmentioning

confidence: 99%

“…After provocation with antigen, mediators, such as prostaglandin D 2, leukotriene E4, tryptase and histamine, are released during the so-called early allergic reaction, which causes sneezing, nasal blockage and rhinorrhoea. After a quiescent period, a late allergic reaction occurs and symptoms recur (Iliopoulos et al, 1990;Naclerio et al, 1983;Pelikan, 1978). Studies on human nasal late responses have shown an influx of eosinophils and an increase in the content of eosinophil cationic protein (ECP) in nasal fluids 3-11 h after challenge.…”

Section: Introductionmentioning

confidence: 99%

“…In our study, the nasal challenge model developed by Naclerio et al (1983) was used to correlate clinical symptoms and the release of biochemical markers such as interleukin-5 and ECP and to establish whether these markers are formed predominantly in the early or late phase of the allergic process in patients with allergic rhinitis. Furthermore, the effect of fluticasone propionate, a new and potent corticosteroid, on the levels of interleukin-5, ECP and on symptom scores after nasal challenge with allergen was investigated.…”

Section: Introductionmentioning

confidence: 99%

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Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients

Garrelds

Veld

Nahori

et al. 1995

European Journal of Pharmacology

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The production of interleukin-5 and eosinophil cationic protein (ECP) in the nasal cavity was examined in 24 patients with rhinitis who were allergic to the house dust mite. During a double-blind placebo-controlled cross-over study, fluticasone propionate aqueous nasal spray (200 tzg) was administered twice daily for 2 weeks. After four basal nasal lavages provocation with house dust mite extract was performed and nasal lavages were collected every hour for 9.5 h. Interleukin-5 was present in detectable amounts in nasal lavages from patients allergic to house dust mite. Nasal challenge with house dust mite extract caused immediate nasal symptoms and increased levels of interleukin-5. Between 3.5 and 8.5 h after the challenge symptoms recurred and interleukin-5 levels increased, reflecting a late phase reaction. Eosinophil cationic protein, a marker of activated eosinophils, was released between 6.5 and 9.5 h after challenge. Treatment with fluticasone propionate (as an aqueous nasal spray) significantly decreased the evoked interleukin-5 and ECP levels in the late phase reaction. This response was correlated with an improved symptom score. This could indicate that the number and activity of eosinophils are increased during the late phase allergic reaction, a response that is inhibited by corticosteroids.

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Section: Nasal Challenge and Lavagementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients

Garrelds

Veld

Nahori

et al. 1995

European Journal of Pharmacology

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“…Para a coleta do fluido nasal (FN), o paciente mantinha a cabeça em extensão em torno de 30°; ocluindo a rinofaringe com o pálato mole, instilava-se fracionadamente 5,5 ml de solução salina em cada narina; após 10 segundos o paciente fletia a cabeça, e o material era coletado em tubo cônico graduado, o qual era mantido no gelo durante o procedimento da coleta e, em seguida, registrava-se o volume recuperado 10,17 .…”

Section: População E Métodosunclassified

Mediadores inflamatórios, citograma em lavado nasal e tomografia computadorizada de seios paranasais em crianças atópicas

Kovalhuk¹,

Carvalho²

2001

J. Pediatr. (Rio J.)

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ResumoObjetivos: os objetivos deste estudo foram avaliar a celularidade e os mediadores inflamatórios no lavado nasal (LN) e a extensão de comprometimento da mucosa dos seios paranasais, em atópicos assintomáticos para sinusite.Métodos: foram estudados 48 atópicos com rinite alérgica (RA), 33/48 com asma; 13 crianças não atópicas constituíram o grupo controle. Foram excluídos os casos com sinusite aguda, crônica ou recorrente. A extensão de lesão da mucosa paranasal, avaliada por tomografia computadorizada (TC) coronal dos seios da face (SF), foi graduada por escore padronizado (0-30). Escore maior ou igual a 12 indicava comprometimento extenso da mucosa dos SF. O LN foi obtido para quantificar a celularidade total e diferencial. Uma alíquota do sobrenadante foi usada para dosagem de mediadores inflamatórios: Interleucina-8 (IL-8), Mieloperoxidase (MPO), Proteína catiônica eosinofílica (ECP). A albumina foi usada como marcador de permeabilidade vascular. Estas determinações foram realizadas em amostras do fluido nasal (FN) de todos os atópicos e em 6/13 dos casos-controle. Foram realizados nos 3 grupos hemograma e espirometria.Resultados: o comprometimento da mucosa paranasal foi extenso em 7/33 (21%) dos asmáticos (Grupo I), 2/15 (13%) dos pacientes com RA (Grupo II). No grupo controle (Grupo III) o maior escore de TC foi 7. A contagem total de células e de eosinófilos/ml e a concentração de albumina do FN foram maiores entre asmáticos com escore de TC acima de 12. O nível de IL-8, o número de neutrófilos e de células epiteliais/ml no FN foram semelhantes nos 3 grupos. Os atópicos (n=48) apresentaram uma correlação positiva entre escore de TC, eosinofilia periférica, número de eosinófilos/ml e concentração de ECP no FN. Houve uma associação entre o número de neutrófilos e concentrações de IL-8 e MPO e entre IL-8 e eosinófilos.Conclusões: em asmáticos assintomáticos para sinusite, o comprometimento extenso da mucosa paranasal está associado à eosinofilia periférica e do lavado nasal e à ativação celular. A infiltração e a ativação de neutrófilos não estavam relacionadas com a maior extensão de lesão da mucosa paranasal.J Pediatr (Rio J) 2001; 77 (4): 271-8: sinusite, asma, rinite, tomografia computadorizada, lavado nasal, interleucina-8, mieloperoxidase, proteína catiônica eosinofílica. AbstractObjective: the aims of this study were to evaluate inflammatory cells, the profile of inflammatory mediators in nasal lavage (NL), and the involvement of the paranasal mucosa in atopic infants with no symptoms of sinusitis.Methods: 48 atopic patients with allergic rhinitis (AR), and 33/ 48 patients with asthma were studied; the control group consisted of 13 nonatopic children. Those individuals with acute, chronic or recurrent sinusitis were excluded. The involvement of the paranasal mucosa was assessed by coronal computed tomography (CT) and graded by a standard protocol (0-30). A CT score greater than or equal to 12 indicated extensive involvement. Nasal lavage was used to quantify total and differential nasal cell coun...

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Fluticasone Nasal Spray and the Combination of Loratadine and Montelukast in Seasonal Allergic Rhinitis

Saengpanich¹,

deTineo²,

Naclerio³

et al. 2003

Arch Otolaryngol Head Neck Surg

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Mediator Release after Nasal Airway Challenge with Allergen

Cited by 373 publications

References 0 publications

Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients

Interleukin-5 and eosinophil cationic protein in nasal lavages of rhinitis patients

Mediadores inflamatórios, citograma em lavado nasal e tomografia computadorizada de seios paranasais em crianças atópicas

Fluticasone Nasal Spray and the Combination of Loratadine and Montelukast in Seasonal Allergic Rhinitis

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