Medical‐Grade Honey Kills Antibiotic‐Resistant Bacteria In Vitro and Eradicates Skin Colonization

Kwakman, Paulus H. S.; Akker, Johannes P. C. van den; Güçlü, Ahmet; Arazi, Hamid; Binnekade, Jan M.; Boer, Leonie de; Boszhard, Laura; Paulus, Frederique; Middelhoek, Pauline; Velde, Anje A. te; Vandenbroucke-Grauls, Christina M. J. E.; Schultz, Marcus J.; Zaat, Sebastian A. J.

doi:10.1086/587892

Cited by 115 publications

(122 citation statements)

References 31 publications

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“…Many in vitro studies have shown antimicrobial activity of different honeys against a wide range of skin-colonizing and foodborne bacterial species, including antibiotic-resistant bacteria (Mundo et al 2004;Lusby et al 2005;Lin et al 2009;Kwakman et al 2008Kwakman et al , 2010. It has been shown that also in vivo honey has beneficial actions against wound infections (Robson et al 2009), and licensed honey products are widely used in wound care (Cooper et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of organic honeys against food pathogenic bacterium Clostridium perfringens

Oinaala

Lehesvaara²,

Lyhs

et al. 2015

Org. Agr.

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Against Clostridium perfringens, a common cause of food poisoning, antimicrobial activity and methyglyoxal (MGO) contents of six multifloral organic honeys were investigated, five from Finland and one from Argentinian and Hungarian origin. For the antimicrobial assessment, a disc diffusion method was used with zone of inhibition expressed as a diameter. Four honeys had inhibitory activity (diameter >8 mm) compared to control (diameter of 6.1 mm±1.5) at the concentration of 50 % (w/v). The highest activity was induced by North Carelien multifloral honey F with willow herb as the main floral source (diameter of 14.3 mm ±0.6), followed by other North Carelien multifloral honey E (diameter of 11.0 mm±2) with clover as the main floral source. For honey F, the minimal inhibitory concentration (MIC) was 20 % (w/v). MGO was quantified by HPLC and varied from 22 to 27 mg/kg. MGO had no correlation to the detected activity. This is a novel finding on the antibacterial activity of native organic honeys and against C. perfringens.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of organic honeys against food pathogenic bacterium Clostridium perfringens

Oinaala

Lehesvaara²,

Lyhs

et al. 2015

Org. Agr.

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“…Therefore, it is crucial to find effective strategies to increase the DO concentration at the bottom of the biofilm and create a more acidic environment. Hyperosmotic concentrations of agents, such as medical-grade honey (48,49) and cadexomer iodine (50,51), have been considered for wound treatment. Recently, it was shown that hyperosmotic concentrations of maltodextrin increased the efficiency of vancomycin against S. aureus biofilms by reducing biofilm coverage, thickness, and diffusion distance, as well as by improving the DO concentration within the biofilm (37).…”

mentioning

confidence: 99%

Hyperosmotic Agents and Antibiotics Affect Dissolved Oxygen and pH Concentration Gradients in Staphylococcus aureus Biofilms

Kiamco

Atçı

Mohamed

et al. 2017

Appl Environ Microbiol

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Biofilms on wound surfaces are treated topically with hyperosmotic agents, such as medical-grade honey and cadexomer iodine; in some cases, these treatments are combined with antibiotics. Tissue repair requires oxygen, and a low pH is conducive to oxygen release from red blood cells and epithelialization. We investigated the variation of dissolved oxygen concentration and pH with biofilm depth and the variation in oxygen consumption rates when biofilms are challenged with medical-grade honey or cadexomer iodine combined with vancomycin or ciprofloxacin. Dissolved oxygen and pH depth profiles in Staphylococcus aureus biofilms were measured using microelectrodes. The presence of cadexomer iodine with vancomycin or ciprofloxacin on the surface of the biofilm permitted a measurable concentration of oxygen at greater biofilm depths (101.6 Ϯ 27.3 m, P ϭ 0.02; and 155.5 Ϯ 27.9 m, P ϭ 0.016, respectively) than in untreated controls (30.1 m). Decreases in pH of ϳ0.6 and ϳ0.4 units were observed in biofilms challenged with medical-grade honey alone and combined with ciprofloxacin, respectively (P Ͻ 0.001 and 0.01, respectively); the number of bacteria recovered from biofilms was significantly reduced (1.26 log) by treatment with cadexomer iodine and ciprofloxacin (P ϭ 0.002) compared to the untreated control. Combining cadexomer iodine and ciprofloxacin improved dissolved oxygen concentration and penetration depth into the biofilm, while medical-grade honey was associated with a lower pH; not all treatments established a bactericidal effect in the time frame used in the experiments.IMPORTANCE Reports about using hyperosmotic agents and antibiotics against wound biofilms focus mostly on killing bacteria, but the results of these treatments should additionally be considered in the context of how they affect physiologically important parameters, such as oxygen concentration and pH. We confirmed that the combination of a hyperosmotic agent and an antibiotic results in greater dissolved oxygen and reduced pH within an S. aureus biofilm.

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“…This is in agreement to the work done by Ball (2007), showing that honey is acidic. Moreover, high osmotic effect (about 80% wt/vol of concentrated sugars) (Ramalivhana et al, 2014) and microorganism sensitivity to hydrogen peroxide (produced by glucose oxidase originating from the bees) found in honey (Ramalivhana et al, 2014;Paulus et al, 2008) constitute inhibitory factors to bacterial growth (Amal, 2014;Manisha and Shyamapada, 2011).…”

Section: Ph Of Samplesmentioning

confidence: 99%

Antibacterial activity of three Cameroonian honey types against some pathogenic species

Teke¹,

Ngienyikeh²

2017

Int. J. Bio. Chem. Sci

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Though much has been documented on the biological activity of honey, no scientific report was seen on the antibacterial activity of honey from Oku, Mbengwi and Ngaoundéré in Cameroon. Our aim was to study the in vitro antibacterial activity of honey produced from these places against clinical isolates. Honey samples were tested for antibacterial activity at concentrations from 100 to 20% volume per volume (v/v) against clinical bacterial isolates using agar well diffusion method. MICs and MBCs of honey samples (49.02 to 1.53% v/v) were determined using broth and agar media respectively. Ciprofloxacin was used as reference substance (120 to 0.5 µg/ml). Honey pH was measured using CYBOW11 test strips. Data were analysed with the help of statistical package for social sciences (SPSS), version 19. These honey samples inhibited the studied bacterial growth at concentrations ≥ 40% with S. aureus and E. coli being more susceptible. Antibacterial activities at concentrations > 60% were comparable to that of the reference drug at p=0.05. The MICs and MBCs ranged from 12.25 to 49.02%. The pH of honeys was estimated to be 5. Oku, Mbengwi and Ngaoundéré honey samples showed antibacterial activity against some bacteria known to cause infectious diseases in humans.

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Medical‐Grade Honey Kills Antibiotic‐Resistant Bacteria In Vitro and Eradicates Skin Colonization

Abstract: Revamil is a promising topical antimicrobial agent for prevention or treatment of infections, including those caused by multidrug-resistant bacteria.

Cited by 115 publications

References 31 publications

Antimicrobial activity of organic honeys against food pathogenic bacterium Clostridium perfringens

Antimicrobial activity of organic honeys against food pathogenic bacterium Clostridium perfringens

Hyperosmotic Agents and Antibiotics Affect Dissolved Oxygen and pH Concentration Gradients in Staphylococcus aureus Biofilms

Antibacterial activity of three Cameroonian honey types against some pathogenic species

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