Medical Polymer-Based Gene Therapy

Jiang, Hu‐Lin; Kim, You-Kyoung; Cho, Chong‐Su; Cho, Myung‐Haing

doi:10.5772/20222

Cited by 2 publications

(2 citation statements)

References 90 publications

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“…Gene therapy is a modern pathway toward demanding disease treatment. 1,2 The most common procedure of gene therapy involves using DNA that encodes a functional, therapeutic gene in order to replace a mutated gene. Toward this end, synthetic polymers have been proposed as delivery agents of DNA.…”

Section: ■ Introductionmentioning

confidence: 99%

Quaternized Poly[3,5-bis(dimethylaminomethylene)hydroxystyrene]/DNA Complexes: Structure Formation as a Function of Solution Ionic Strength

2013

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The formation of complexes between the cationic polyelectrolyte poly[3,5-bis(dimethylaminomethylene)hydroxystyrene] (Q-N-PHOS), bearing two cationic sites per repeating unit, and DNA molecules in aqueous solutions is investigated at pH 7 and at various salt (NaCl) concentrations and DNA/polymer ratios. The structural characteristics of the polyplexes at different DNA/polymer ratios were characterized in terms of mass, size, and charge using static, dynamic, and electrophoretic light scattering and fluorescence spectroscopy. The results indicate that the complexes have a loose spherical morphology with size in the range of 45-100 nm depending on polymer to DNA ratio and ionic strength of the solution. Most interestingly, it is found that the polyplexes' response to changes in the ionic strength of the surrounding solution after complexation depends strongly on the initial solution ionic strength during complex formation. This also points to differences in the nanostructures of polyplexes formed at different ionic strengths.

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Section: ■ Introductionmentioning

confidence: 99%

Quaternized Poly[3,5-bis(dimethylaminomethylene)hydroxystyrene]/DNA Complexes: Structure Formation as a Function of Solution Ionic Strength

2013

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“…Chitosan is one of that can generally consider as biocompatible and biodegradable polycationic polymer, which has minimum immunogenicity and low cytotoxicity (7). Therefore, chitosan and its derivatives may represent potentially safe cationic carriers for use in gene delivery (8). Factors include; degree of deacetylation, molecular weight, and charge of chitosan, and pH media, are significant role in the determination of the transfection efficiency of polyplexes containing chitosan and DNA (9).…”

Section: Introducctionmentioning

confidence: 99%

The effect of synthesized chitosan grafted poly (N-L-lactide) on human genetic material

et al. 2018

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Introduction: Chitosan is one of the natural polymers can generally consider as a biocompatible and biodegradable polycationic polymer, which has minimum immunogenicity and low cytotoxicity. Therefore, chitosan and its derivatives may represent potentially safe cationic carriers for use in gene delivery.Materials and Methods: Chitosan with 90.1 DD% obtained by deacetylation of chitin extracted from local shrimp shells. Graft copolymerization of L-lactide onto chitosan was carried out at room temperature by ring opening polymerization under a nitrogen atmosphere to prepare chitosan-g-poly (N-lactide) graft copolymer. It was obtained in good yield and characterized by FTIR. The samples purity and concentration were detected using both Nanodrop UV-spectroscopy and agarose gel electrophoresis techniques. The human heat shock proteins gene, hsp-70, was used as a model of human genes to study the effect of chitosan-g-poly(N-lactide) graft copolymer. Results and Discussion: The results revealed that chitosan-g-poly (N-lactide) graft copolymers had safety effect on the DNA, and binding with it. the human heat shock proteins gene, hsp-70, was used as a model of human genes to study the effect of chitosan-g-poly(N-lactide) graft copolymer, it shows a good binding ability the human gene, implies that it might be used in biomedical applications in the future. Conclusions: Grafting of L-lactide onto chitosn by ring opening polymerization was confirmed by FTIR. The repaired polymer has safety effects on human DNA and genes. The chitosan-g-poly (N-lactide) graft copolymer has shown high efficiency to electrostatic interaction with human DNA and gene, implying that it is suitable to be used as DNA and gene delivery.

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Medical Polymer-Based Gene Therapy

Cited by 2 publications

References 90 publications

Quaternized Poly[3,5-bis(dimethylaminomethylene)hydroxystyrene]/DNA Complexes: Structure Formation as a Function of Solution Ionic Strength

Quaternized Poly[3,5-bis(dimethylaminomethylene)hydroxystyrene]/DNA Complexes: Structure Formation as a Function of Solution Ionic Strength

The effect of synthesized chitosan grafted poly (N-L-lactide) on human genetic material

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