Medical significance of simultaneous application of red blood cell distribution width (RDW) and neopterin as diagnostic/prognostic biomarkers in clinical practice

Zvetkova, E.; Fuchs, Dietmar

doi:10.1515/pterid-2017-0021

Cited by 9 publications

(10 citation statements)

References 81 publications

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“…RBC indices and anemia are precursors for HIV-1-associated neurocognitive disorders . Furthermore, the ageing of RBCs and their disposition in the spleen are portent by spicules formed on the cell surface (echinocytes). , Thus, the absence of morphological changes in RBCs is a prerequisite in HIV. The SEM image of RBCs treated with Efa–Enf Co-loaded PLN, blank PLN, and normal saline (negative control) portrayed a healthy biconcave-shaped RBC morphology, while positive control images revealed an irregular morphology with pore formation and echinocytes.…”

Section: Resultsmentioning

confidence: 99%

Long-Acting Efavirenz and HIV-1 Fusion Inhibitor Peptide Co-loaded Polymer–Lipid Hybrid Nanoparticles: Statistical Optimization, Cellular Uptake, and In Vivo Biodistribution

et al. 2020

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The objective of the present study was to develop long-acting efavirenz (Efa)−enfuvirtide (Enf) Co-loaded polymer−lipid hybrid nanoparticles (PLN) with improved intracellular delivery to target T-cells and macrophage cells located in multiple human immunodeficiency virus sanctuaries. The Box−Behnken design was utilized to optimize three high-risk factors, namely, Efa amount, sonication time for primary emulsion, and sonication time for aqueous nanodispersion obtained from preliminary studies. Lyophilized Efa−Enf Co-loaded PLN using trehalose elicited spherical morphology, drug amorphization on incorporation, and absence of drugexcipient interaction. In vitro release studies revealed an sustained release of both the drugs from PLN with the differential release profile. Efa−Enf Coloaded PLN exhibited low hemolytic, platelet and leukocyte aggregation as well as low cytotoxicity in Jurkat E6.1 T-cells and U937 macrophage cells. Circular dichroism spectra confirmed the presence of an α-helix form of Enf after encapsulation in PLN. Coumarin-6-loaded PLN exhibited enhanced cellular uptake in Jurkat E6.1 T-cells and U937 macrophage cells in comparison to free coumarin-6, as evidenced by fluorescence microscopy and flow cytometry. In vivo biodistribution studies after intravenous administration of near-infrared dye-loaded PLN (surrogate for Efa−Enf PLN) revealed non-uniform distribution within 2 h in the order of spleen ≥ liver > lymph node > thymus > lungs > female reproductive tract (FRT) > heart > kidneys > brain. However, subcutaneous administration caused non-uniform biodistribution after 3 days, eliciting a long-acting slow release from the injection site depot until day 5 in the infection-spread site (lymph nodes and FRT), reservoir sites (liver and spleen) and the difficult-to-access site (brain). Furthermore, it presents a vital illustration of the available tissue-specific drug concentration prediction from simulated surrogate PLN.

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Section: Resultsmentioning

confidence: 99%

Long-Acting Efavirenz and HIV-1 Fusion Inhibitor Peptide Co-loaded Polymer–Lipid Hybrid Nanoparticles: Statistical Optimization, Cellular Uptake, and In Vivo Biodistribution

et al. 2020

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“…Many studies have revealed that RDW is also associated with liver, kidney, and cardiovascular diseases. In addition, RDW is known to be a recently used inflammation marker in inflammatory diseases, such as septic shock, inflammatory bowel disease, and acute appendicitis (19,(35)(36)(37). In addition, RPR has been shown to be a new and rapid laboratory index for predicting mortality in many diseases.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Relationship between Simple Hemogram Indexes and Disease Severity Scores in Pediatric Familial Mediterranean Fever

Güngörer

Arslan²

2022

Düzce Tıp Fakültesi Dergisi

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Aim:In recent years, it has been seen that simple complete blood count (CBC) parameters can be used to show subclinical inflammation in patients with familial Mediterranean fever (FMF). The aim of this study is to determine whether there is a difference in CBC parameters among FMF patient groups divided according to disease severity scores. Material and Methods: FMF patients followed up in our clinic between 2016 and 2019, were reviewed for medical records. They were divided into three groups as those with mild, moderate, and severe diseases according to the disease severity scoring systems by Pras et al., Mor et al., and International Severity Score of FMF (ISSF). Red cell distribution width (RDW), platelet, neutrophil, lymphocyte, RDW-platelet ratio (RPR), platelet-lymphocyte ratio (PLR), and neutrophil-lymphocyte ratio (NLR) of the patients were compared among the groups. Results: According to the scoring system of Pras et al., lymphocyte value was found significantly higher in the group with severe disease compared to the groups with mild and moderate diseases (p=0.031). PLR was significantly lower in the group with severe disease compared to moderate diseases according to the scoring system of Mor et al (p=0.008). According to ISSF, there was no difference among the groups in terms of CBC parameters. Conclusion: Different results were obtained according to all three scoring systems. Since the ISSF is the most common and suitable system for use in FMF patients, we can conclude that there is no relationship between disease severity and RDW, RPR, NLR and PLR.

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“…Others have previously posited RDW to be indicative of poor cancer prognosis. [21][22][23][24][25] A multivariable analysis of the data showed recurrences correlated with age and pathologic T stage, but not RDW (Table 4).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Chest Radiographs and Laboratory Testing during Melanoma Staging Procedures

Votanopoulos

Russell

et al. 2019

The American Surgeon™

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Chest radiographs (CXRs) and laboratory testing have historically been performed as a part of low-risk melanoma (clinical stage 1/2) workup. This study evaluates the utility of routine CXRs and laboratory testing during the staging of clinical stage 1 and 2 melanoma patients. This study was approved by the Institutional Review Board at Wake Forest University. A database of sentinel lymph node biopsies performed for clinical stage 1 or 2 melanoma was used to identify early-stage melanoma patients. The medical records of patients with melanoma were reviewed and pre-operative workup procedures were recorded. Four hundred sixty-three patients were reviewed. A total of 315 patients underwent a preoperative CXR, whereas 309 received some laboratory testing. After sentinel node biopsies, 168 patients had pathologic stage 1 disease, 103 stage 2, and 44 stage 3. None of the CXRs (0%) correctly identified metastatic melanoma. Suspicious locations on CXRs and laboratory testing did not lead to metastatic findings in any patient within a year. Metastatic melanoma was not found in any patient by screening with CXRs or laboratory testing during preoperative workup. We recommend not conducting CXRs or laboratory testing during workup for surgical melanoma patients because of charges and anxiety these tests can cause. CXRs, blood tests, and metabolic panels have historically been ordered for early melanoma patients, although debate remains on their efficacy. Surgical patient records were retrospectively reviewed for these tests and no benefit was found.

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Medical significance of simultaneous application of red blood cell distribution width (RDW) and neopterin as diagnostic/prognostic biomarkers in clinical practice

Cited by 9 publications

References 81 publications

Long-Acting Efavirenz and HIV-1 Fusion Inhibitor Peptide Co-loaded Polymer–Lipid Hybrid Nanoparticles: Statistical Optimization, Cellular Uptake, and In Vivo Biodistribution

Long-Acting Efavirenz and HIV-1 Fusion Inhibitor Peptide Co-loaded Polymer–Lipid Hybrid Nanoparticles: Statistical Optimization, Cellular Uptake, and In Vivo Biodistribution

Evaluation of the Relationship between Simple Hemogram Indexes and Disease Severity Scores in Pediatric Familial Mediterranean Fever

Evaluation of Chest Radiographs and Laboratory Testing during Melanoma Staging Procedures

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