Medicinal Plants used in the Treatment of Hepatitis in BoboDioulasso: Studying the Availability and Analyzing the Phytochemical Properties of Combretum micranthum G. Don and Entada africana Guill. et Perr.

Abstract

This paper focuses on studying the inventory of the medicinal plants used in hepatitis care in Bobo-Dioulasso and evaluating their availability in local vegetation and their phytochemical properties. To achieve this objective, several approaches were developed which include: (1) an ethnobotanical survey among 111 traditional health practitioners (THP); (2) a dendrometric study to evaluate the abundance and spatial distribution of these species in Dindérésso Classified Forest; and (3) an evaluation of polyphenolic compounds and antioxidant activity of the two most quoted species roots using three methods such as anti-DPPH*, anti-FRAP, and antiABTS. The results show that Entada africana and Combretum micranthum were the most quoted species among 40 species used in the treatment of liver disease. The availability study revealed that juvenile and adult individuals of E. africana are frequent in woody savannah, shrubby savannah, and grassy savannah. As for juvenile and adult C. micranthum individuals, they are only frequent in wooded savannah (RI<60%). These populations are therefore declining due to anthropogenic pressure. Phytochemical analysis reveal polyphenols contents of 37.91 and 20.71 mg EAG/100 mg respectively for C. micranthum and E. africana and flavonoids contents of 0.85 ± 0.09 and 0.66 ± 0.05 mg EQ/100 mg respectively for C. micranthum and E. africana. Finally, the results show that there is an anti-oxidant activity for the two species. There were about 198 and 13 μmol EAA/g for the two species i.e., ABTS and DPPH, respectively. The results show that the antioxidant activity could partially justify the traditional use of this plant.

Ethnobotanical Survey on Medicinal Plants Used in Burkina Faso in the Treatment of Breast Cancer, Phytochemistry and Antioxidant Activities: Euphorbia poissonii Pax and Flueggea virosa (Willd.) Voigt. (Euphorbiaceae)

“…The FRAP (Ferric Reducing Antioxidant Power) method is based on the ability of the extracts to reduce ferric ion (Fe 3+ ) to ferrous ion (Fe 2+ ). The total antioxidant capacity of each plant extract was determined by the method described by Dakio et al (2020).The sample solution was prepared with distilled water to 0.5 mL of sample solution, followed by 1.25mL of phosphate buffer (0.2 M) and 1.25mL of potassium hexacyanoferrate were added. This mixture was incubated for 30 minutes in a water bath at 50 • C. After that, 1.25 mL of trichloroacetic acid (10%) was added and the whole centrifuged for 10 minutes at 300 rpm.…”

“…The antiradical activity of the extracts by the DPPH method, is their ability to scavenge the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH • ). The method used was as described by Dakio et al (2020). 375 μL of methanolic sample solution is mixed with 750 μL of DPPH solution (20 mg/L).…”

“…The method used is the one described by Dakio et al (2020).The sample solution is prepared with distilled water. 990 μL of ABTS the standard (y = -0.0007874x + 0.709; R 2 = 0.9993).…”

“…Determination of total polyphenols: The estimation of total extractable phenolic compounds was performed by the Folin-Ciocalteu method described by Dakio et al (2020). The sample solution diluted to one hundredth from the stock solution was used.…”

“…The method used for the estimation of flavonoid levels in plant extracts and fractions is that described by Dakio et al (2020). The sample solution diluted to the hundredth was used to perform the operation.…”