Medicinal prospective of Citrus limon and Citrus sinensis peels essential oil by Gas Chromatography/ Mass Spectrometry (GC/MS) compositional analysis

Uraku, A. J.; Uraku, O. H.; Nwankwo, V. O. U.; Okoye, C. J.; Ozioma, P. E.; Edenta, C.; Ezeali, C.

doi:10.31248/jbbd2020.129

Cited by 1 publication

(1 citation statement)

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“…The findings of the present work are in a good agreement with previous reports which reported limonene (70-92.5 %) as a major component of C. sinensis peels (Mursiti et al, 2019). Uraku et al(2020) reported five compounds from GC-MS analysis of methanolic extract of C. sinesis peel such as pyridine-2-carbaldehyde, 1-Methyl-1H-pyrrole-2-carbaldehyde (40%), glutamic acid (25%), 2-ethyl-5-methyl-1H-pyrrole (14%) and pyrrolidin-2-one (12%).…”

Section: Essential Oil Chemical Compositionsupporting

confidence: 93%

Antibacterial and antioxidant activity of compounds from Citrus sinensis peels and in silico molecular docking study

YOHANES,

GEREMEW,

TAFESE

et al. 2023

International Journal of Secondary Metabolite

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The increasing prevalence of drug resistance, adverse side effects of existing antibiotics, and the resurgence of previously known infections have necessitated the search for new, safe, and effective antimicrobial agents. The peels of Citrus sinensis (300 g) were extracted using maceration and ultrasonic-assisted extraction methods with ethanol, resulting in yields of 20.99 g and 11.5 g (7%, 7.5%), respectively. Silica gel column chromatographic separation of the ethanol extract yielded N-(1,3,4,5-tetrahydroxydecan-2-y1)octanamide (1), decanoic acid (2), β-sitosterol-3-O-β-D-glucopyranoside derivative (3), and (z)-ethyl tetradec-7-enoate (4). GC-MS analysis of the essential oil detected 7 chemical components accounting for 99.84% of the total composition of which limonene was found to be the predominant constituent (87.5%). In vitro antibacterial tests revealed promising zones of inhibition by ethanol extract (12.67±0.58 mm, at 150 mg/mL), compound 4 (15.67±2.88 mm, at 6 mg/mL), and compound 1 (12.00±0.00 mm, at 6 mg/mL) against E. faecalis, S. typhimurium, and P. aeruginosa, respectively, compared to gentamicin (13.00±1.73 mm, 18.00±1.00 mm, and 16.67±1.15 mm, respectively at 10 µg/mL). DPPH radical scavenging activity indicated that compound 1 exhibited an IC50 value of 0.05 mg/mL, compared to ascorbic acid's 0.016 mg/mL. In silico molecular docking studies revealed that compounds 1 and 3 had the lowest scoring poses against E. coli DNA gyrase B enzyme, human peroxiredoxin 5, and S. aureus pyruvate kinase, respectively. These findings support traditional applications of Citrus peels in treating infectious diseases, particularly against Gram-positive strains, and highlight their potential use as antibacterial ingredients in cosmetics.

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Section: Essential Oil Chemical Compositionsupporting

confidence: 93%