Mediterranean agro‐industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid‐state fermentation

Sousa, Daniel; Venâncio, Armando; Belo, Isabel; Salgado, José Manuel

doi:10.1002/jsfa.9063

Cited by 44 publications

(31 citation statements)

References 36 publications

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“…Crude protein is an important component as this substrate may be used as animal feed. No significant differences among samples were observed, which is in contrast with the results of Sousa et al [31], who found an increase in protein content, but by using different plant wastes fermented by different fungal species.…”

Section: Pretreatment: Lignocellulose Composition Enzymatic Activiticontrasting

confidence: 99%

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Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

Fernandes

Fraga

Sousa

et al. 2020

IJERPH

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Grape stalks, an inedible lignocellulosic residue from winemaking and agro-industrial grape juice production, can be valorized as a source of bioactive compounds and as feedstock for the saccharification and bioconversion of soluble sugars. Solid-state fermentation (SSF) by six white-rot fungi was applied as pretreatment. Fiber composition, free radical scavenging activity, four ligninolytic, and three hydrolytic enzyme activities were determined. Saccharification kinetics, yield, and productivity were evaluated and complemented with scanning electron microscopy (SEM), high performance liquid chromatography (HPLC) quantification of monosaccharides, and principal component analysis (PCA). After SSF, the biomass exhibited a drastic free radical scavenging activity decrease and the main enzymes produced were manganese-dependent peroxidase and xylanase. Scanning electron microscopy revealed the erosion of cell walls, and PCA exhibited a negative correlation between saccharification, and neutral detergent fiber and acid detergent lignin. Phlebia rufa pretreated biomass gave the highest sugars yield and productivity, representing a nearly three-fold increase compared to untreated samples. Also, monosaccharides quantification revealed that the 1:1 ratio of glucose to the sum of xylose plus galactose changes to the value of 2:1 after pretreatment. In this work, and for the first time, P. rufa proved to be an effective pretreatment of grape stalks for the saccharification and further bioconversion into value-added chemicals. In addition, lignocellulolytic enzymes were also produced through SSF.

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Section: Pretreatment: Lignocellulose Composition Enzymatic Activiticontrasting

confidence: 99%

“…contrast with the results of Sousa et al [31], who found an increase in protein content, but by using different plant wastes fermented by different fungal species.…”

Section: Pretreatment: Lignocellulose Composition Enzymatic Activiticontrasting

confidence: 93%

Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

Fernandes

Fraga

Sousa

et al. 2020

IJERPH

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“…OMSW is a lignocellulosic byproduct characterized by its high organic content and phenolic compounds. There are several experimental treatments for OMSW, which have the potential to make it re-usable as fertiliser and water for irrigation or to obtain valuable materials from it such as, proteins, minerals and polysaccharides (Pantziaros et al 2018;Sousa et al 2018). However, the reuse of OMSW is restricted by the abundance of complex compounds it contains and its elevated polluting load.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic co-digestion of olive mill solid waste and microalga Scenedesmus quadricauda: effect of different carbon to nitrogen ratios on process performance and kinetics

et al. 2019

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Anaerobic digestion (AD) is one of the most efficient processes for treating agri-food waste in order to obtain renewable energy. Olive mill solid waste (OMSW) is the main residue from the two-phase olive oil manufacturing process; it has a high organic content and high C/N ratio, which hinders its AD, giving low methane yield. In the present study, a microalga, Scenedesmus quadricauda (S. quadricauda), was used as co-substrate for the AD of OMSW to compensate for its nitrogen deficiency. The robustness and the high growth rate of S. quadricauda make this microalga a potential source of nitrogen to co-digest with carbonrich substrates. Different co-digestion mixtures of OMSW-microalgae and the single substrate were tested. For all co-digestion mixtures, the alkalinity value at the end of the experiment remained below 4889±245 mg CaCO 3 /L and pH in the range of 7.50-7.67 indicating stability and good process performance. The results showed the highest methane yield (461 mL CH 4 2 STP/g VS added) for the co-digestion mixture 75% OMSW-25% S. quadricauda (C/N=25.3), which was 104 and 23% higher than that obtained from the single microalga (C/N=5.6) and OMSW (C/N=31.9), respectively. No ammonia inhibition was detected despite the high protein content of the microalgae. The transference function model allowed for adequately fitting the experimental results of methane production with time in the anaerobic experiments.The highest maximum methane production rate, R m , among the different co-digestion mixtures assayed was obtained for the mixture 75% OMSW-25% S. quadricauda with a value of 89 mL CH 4 /(g VS d).

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“…Agroindustrial wastes such as wheat/rice bran are used by microorganisms as finicky substrates in SSF for production of industrial enzymes and biopharmaceuticals due to their porous structure amenable for growth and capacity to hold requisite moisture [8]. Production of a multitude of industrial enzymes such as cellulases and xylanases [29], amylases [2,23], proteases [19] using SSF and agroindustrial wastes has been reported by earlier researchers. Fish scales are chitin loaded and can be used as substratum to produce chitinolytic microbial enzymes [11,14].…”

Section: Introductionmentioning

confidence: 99%

Anticoagulant activity of partially purified chitinase produced by Citrobacter freundii str. nov. haritD11 by fermentation of wheat bran coupled with fish scales

Meruvu

2018

SN Appl. Sci.

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Solid phase fermentation of wheat bran coupled with powdered fish scales could yield chitinase using a novel Citrobacter strain. The fermentation conditions are optimized conventionally (84.14 U/gds) and statistically (94.3 U/gds). The partially purified chitinase had a specific activity of 53.41 U/mg with optimal activity at pH 8.5 and 50 °C, it was stable at 7.5-10 pH range for 24 h with more than 80% stability at 45-60 °C, and the Km value of chitinase with swollen chitin (substrate) is 8.11 mg/ml with a V max of 2.43 mmol h −1 ml −1 . The partially purified enzyme was halotolerant showing maximum activity and stability up to 10% of Nacl, also possessing potential anticoagulant activity. This is the first report to date elucidating the production of halotolerant chitinase from wheat bran supplemented with fish scales using Citrobacter freundii haritD11 and its application as an anticoagulant agent and notable tolerance to heavy metal ions.

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Mediterranean agro‐industrial wastes as valuable substrates for lignocellulolytic enzymes and protein production by solid‐state fermentation

Abstract: The nutritional value of Mediterranean wastes was improved and other value-added products such as lignocellulolytic enzymes were produced in the same process, which could facilitate the efficient reuse of these wastes. © 2018 Society of Chemical Industry.

Cited by 44 publications

References 36 publications

Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

Pretreatment of Grape Stalks by Fungi: Effect on Bioactive Compounds, Fiber Composition, Saccharification Kinetics and Monosaccharides Ratio

Anaerobic co-digestion of olive mill solid waste and microalga Scenedesmus quadricauda: effect of different carbon to nitrogen ratios on process performance and kinetics

Anticoagulant activity of partially purified chitinase produced by Citrobacter freundii str. nov. haritD11 by fermentation of wheat bran coupled with fish scales

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