Mediterranean Fruit Flies: Sterility and Sexual Competitiveness in the Laboratory After Treatment with Gamma Irradiation in Air, Carbon Dioxide, Helium, Nitrogen or Partial Vacuum1

Ohinata, K.; Ashraf, Mohammad; Harris, E. J.

doi:10.1093/jee/70.2.165

Cited by 37 publications

(30 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…ROS generated during and after irradiation continue to induce other forms of cellular damage that ultimately lead to poor mating competitiveness of irradiated males compared with non-irradiated males (Calkins and Parker, 2005;Nestel et al, 2007). For example, irradiated male Mediterranean fruit flies (medflies) show reduced flight performance, female attraction and mating success compared with unirradiated males (Hooper, 1971;Ohinata et al, 1977;Nestel et al, 2007). Furthermore, many tephritid fruit flies, like caribflies and medflies, engage in highly competitive lek mating, wherein males aggregate together and display to attract females (Robinson et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Short-term anoxic conditioning hormesis boosts antioxidant defenses, lowers oxidative damage following irradiation and enhances male sexual performance in the Caribbean fruit fly, Anastrepha suspensa

López‐Martínez

Hahn

2012

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYMost organisms are repeatedly exposed to oxidative stress from multiple sources throughout their lifetimes, potentially affecting all aspects of organismal performance. Here we test whether exposure to a conditioning bout of anoxia early in adulthood induces a hormetic response that confers resistance to oxidative stress and enhances male sexual performance later in life in the Caribbean fruit fly, Anastrepha suspensa. Anoxic conditioning of adults prior to emergence led to an increase in antioxidant capacity driven by mitochondrial superoxide dismutase and glutathione peroxidase. When exposed to gamma irradiation, a strong oxidative stressor, males that received anoxic conditioning had lower lipid and protein oxidative damage at sexual maturity. Anoxia conditioning led to greater male sexual competitiveness compared with unconditioned males when both were irradiated, although there was no effect of anoxia conditioning on mating competitiveness in unirradiated males. Anoxia also led to higher adult emergence rates and greater flight ability in irradiation-stressed flies while preserving sterility. Thus, hormetic treatments that increased antioxidant enzyme activity also improved male performance after irradiation, suggesting that antioxidant enzymes play an important role in mediating the relationship between oxidative stress and sexual selection. Furthermore, our work has important applied implications for the sterile insect technique (SIT), an environmentally friendly method of insect pest control where males are sterilized by irradiation and deployed in the field to disrupt pest populations via mating. We suggest that hormetic treatments specifically designed to enhance antioxidant activity may produce more sexually competitive sterile males, thus improving the efficacy and economy of SIT programs. Supplementary material available online at

show abstract

Section: Introductionmentioning

confidence: 99%

Short-term anoxic conditioning hormesis boosts antioxidant defenses, lowers oxidative damage following irradiation and enhances male sexual performance in the Caribbean fruit fly, Anastrepha suspensa

López‐Martínez

Hahn

2012

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…Therefore, the number of fractionated doses to be used should take into consideration the release schedule of the sterile insects and the risk of human error. Environmental conditions influencing the repair of DNA damage, such as low temperature or low‐oxygen levels during and after irradiation, are also important because these factors affect cellular radiation sensitivity (Ohinata et al., 1977; Rananavare et al., 1991; Fisher, 1997). Fractionated‐dose irradiation can affect the level of reproductive sterility even if the total receiving dose was equal to a single‐dose irradiation.…”

Section: Discussionmentioning

confidence: 99%

Prolongation of the effective copulation period by fractionated‐dose irradiation in the sweet potato weevil, Cylas formicarius

Kumano

Kuriwada

Shiromoto

et al. 2011

Entomologia Exp Applicata

View full text Add to dashboard Cite

The sterile insect technique (SIT), based on the principles of population and behavioral ecology, is widely used to suppress or eradicate target pest insect populations. The effectiveness of SIT depends on the ability of released sterile males to mate with and inseminate wild females; however, the use of gamma radiation to induce sterility negatively affects both somatic cells as well as reproductive cells. Consequently, sterilization by irradiation drastically diminishes mating performance over time. It is well known that fractionated‐dose irradiation, in which a sterilizing dose is delivered via a series of smaller irradiations, reduces radiation damage. In the present study, we evaluated the effect of fractionated‐dose irradiation on fertility, longevity, and mating propensity in Cylas formicarius (Summers) (Coleoptera: Brentidae) for 16 days after irradiation. Fractionated‐dose irradiation with 200 Gy induced full sterility regardless of the number of radiation doses. Although the mating propensity of males sterilized by a single 200 Gy dose (the current standard of the Okinawa Prefecture SIT program) was equal to that of non‐irradiated weevils for the first 6 days, the mating propensity of males sterilized by a series of three doses was maintained for at least the first 12 days. These results demonstrated that fractionated‐dose irradiation can be highly advantageous in C. formicarius eradication programs.

show abstract

“…There are many steps in the irradiation process and these steps need to be managed to maximize insect quality during the overall process. It is thought that low oxygen tension in pupal tissue reduces the formation of toxic free radicals and peroxides during irradiation (Ohinata et al., 1977). Although this is an accepted technology in most mass rearing and sterilization facilities (FAO/IAEA/USDA 2003), the mechanism still has not been fully investigated despite considerable research into irradiation under low oxygen conditions (Nestel et al., 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Subsequently, the nitrogen technique was developed for Dacus cucumis (French) (Hooper, 1976). Experimentation continued to examine atmospheric pre‐conditioning of medfly to minimize the adverse effects of irradiation because nitrogen flushing and similar techniques are generally only used where rearing and irradiation occur at the same location (Ohinata et al., 1977; Nestel et al., 2007). A simpler method to decrease the level of oxygen in pupal tissue was to seal pupae in plastic bags (bag method) (Nestel et al., 2007).…”

Section: Introductionmentioning

confidence: 99%

Effect of conditions in sealed plastic bags on eclosion of mass‐reared Queensland fruit fly, Bactrocera tryoni

Dominiak

Sundaralingam

Jiang

et al. 2011

Entomologia Exp Applicata

View full text Add to dashboard Cite

The sterile insect technique has been used for more than 50 years to control a range of insects around the world. Sterile insect technique is rapidly becoming a major component of many area‐wide fruit fly management programmes. Irradiation of immature life stages induces sterility in adults, which are then distributed over large areas to mate with wild flies, resulting in no viable offspring. However, irradiation in normal air results in declining adult quality. To optimize the quality of sterile adult flies, several techniques are available to lower the levels of oxygen in fruit fly tissues prior to irradiation. The simplest method is to seal pupae in plastic bags and allow the oxygen consumption of pupae to minimize oxygen in both the air and pupal tissue. Some fruit fly species have rapid decreases in eclosion as a result of low oxygen atmospheres. We tested the tolerance of Queensland fruit fly, Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), to low oxygen for the first time. In the first two experiments, unirradiated B. tryoni pupae were tested for different periods in sealed plastic bags at 17, 21, and 26 °C. Optimum eclosion occurred at 21 °C with the lowest eclosion at 26 °C. In general, mean full eclosion declined at ca. 0.1% eclosion per hour sealed in plastic bags during the first 96 h for all temperatures. In the third and fourth experiments at 17 °C, there was a decline in average eclosion for irradiated and unirradiated pupae of about 13.4% after they were sealed in plastic bags for 192 h. In general, B. tryoni eclosion declined at 0.1% per hour inside sealed plastic bags for periods up to 192 h at 17 °C. Queensland fruit flies can tolerate long periods of conditions found inside sealed plastic bags and current practices for sterile B. tryoni release programmes will result in minimum decrease in eclosion. The possible evolution of tolerance of these conditions is discussed.

show abstract

Mediterranean Fruit Flies: Sterility and Sexual Competitiveness in the Laboratory After Treatment with Gamma Irradiation in Air, Carbon Dioxide, Helium, Nitrogen or Partial Vacuum1

Cited by 37 publications

References 0 publications

Short-term anoxic conditioning hormesis boosts antioxidant defenses, lowers oxidative damage following irradiation and enhances male sexual performance in the Caribbean fruit fly, Anastrepha suspensa

Short-term anoxic conditioning hormesis boosts antioxidant defenses, lowers oxidative damage following irradiation and enhances male sexual performance in the Caribbean fruit fly, Anastrepha suspensa

Prolongation of the effective copulation period by fractionated‐dose irradiation in the sweet potato weevil, Cylas formicarius

Effect of conditions in sealed plastic bags on eclosion of mass‐reared Queensland fruit fly, Bactrocera tryoni

Contact Info

Product

Resources

About