Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage ( Brassica campestris L. ssp. pekinensis )

Zhang, F.-L.; Takahata, Yoshihito; Xu, Jiantang

doi:10.1007/s002990050482

Cited by 75 publications

(44 citation statements)

References 20 publications

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“…Solan green head). In vitro regeneration is influenced by many factors such as culture environment, culture medium composition, explant source, plant growth regulators and genotype (Zhang et al 1998;Rajicic et al 2002;Bano et al 2010;Jana and Shekhawat 2010;Dhir and Shekhawat 2014). In the present study, young tender completely green leaves and petiole explants from in vivo grown seedlings and completely green fully expanded cotyledons and hypocotyl which were greenish in colour and turgid nature from10-12 days old seedlings were used for efficient shoot regeneration studies.…”

Section: Resultsmentioning

confidence: 91%

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Kumar

Srivastava

2015

Physiol Mol Biol Plants

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Broccoli (Brassica oleracea L. var. italica) is an important, nutritionally rich vegetable crop, but severely affected by environmental stresses, pests and diseases which cause massive yield and quality losses. Genetic manipulation is becoming an important method for broccoli improvement. In the present study, a reproducible and highly efficient protocol for obtaining organogenesis from hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica cv. Solan green head) has been developed. Hypocotyl and cotyledon explants were used from 10 to 12 days old aseptically grown seedlings whereas leaf and petiole explants were excised from 18 to 20 days old green house grown seedlings and surface sterilized. These explants were cultured on shoot induction medium containing different concentration and combination of BAP and NAA. High efficiency shoot regeneration has been achieved in hypocotyl (83.33 %), cotyledon (90.11 %), leaf (62.96 %) and petiole (91.10 %) explants on MS medium supplemented with 3.5 mg/l BAP+ 0.019 mg/l NAA 2.5 mg/l BAP+0.5 mg/l NAA, 4.0 mg/l BAP+0.5 mg/l NAA and 4.5 mg/l BAP+0.019 mg/l NAA respectively. Petiole explants showed maximum shoot regeneration response as compared to other explants. MS medium supplemented with 0.10 mg/l NAA was found best for root regeneration (100 %) from in vitro developed shoots. The regenerated complete plantlets were transferred to the pots containing cocopeat and successfully acclimatized. This optimized regeneration protocol can be efficiently used for genetic transformation in broccoli. This is the first comparative report on multiple shoot induction using four different types of explants viz. hypocotyl, cotyledon, leaf and petiole.

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Section: Resultsmentioning

confidence: 91%

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Kumar

Srivastava

2015

Physiol Mol Biol Plants

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“…In vitro regeneration in Brassica ssp. is highly genotype-dependent as reported in previous studies for B. napus [15,16], B. juncea [17], B. rapa [18] and B. oleracea [19]. In addition, Dietert et al [20] compared 6 species of the genus Brassica for callus growth and plant regeneration and reported a high influence of the genotype, with as much inter-cultivar as inter-species differences in the response to the in vitro culture.…”

Section: Introductionmentioning

confidence: 61%

“…This result agreed with studies in Brassica spp., which also reported that shoot production was highly variable among different genotypes, indicating that the regeneration ability is strongly influenced by genotype [15][16][17][18][19][20]. Zhang et al [18] reported a maximum of 10.7 shoots per explant in a cultivar of Chinese cabbage. In our study the maximum number of shoots per explant was 8.7 and 10.4, regenerated respectively from callus and direct regeneration, both by genotype 31.…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Collection of Brassica carinata Genotypes for in vitro Culture Response and Mode of Shoot Regeneration

Gil-Humanes

Martı́n²,

Barro³

2011

AJPS

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“…oleifera (Burnett et al 1994), Brassica campestris ssp. pekinensis (Chi et al 1991;Zhang et al 1998), hypocotyls of Brassica juncea (Pua and Chi 1993) and Raphanus sativus (Pua et al 1996), peduncle and leaf segments of Brassica napus (Eapen and George 1997;Akasaka-Kennedy et al 2005). AgNO 3 is a potent inhibitor of ethylene action, and ethylene is considered to suppress shoot morphogenesis in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…AgNO 3 is a potent inhibitor of ethylene action, and ethylene is considered to suppress shoot morphogenesis in vitro. Zhang et al (1998) Shoot regeneration in B. juncea L. is highly variable and genotype specific. Genotypic influence on in vitro morphogenesis has been documented previously on in B. juncea (Chi et al 1990), B. napus, B. campestris and R. sativus L. (Ono et al 1994;Takasaki et al 1996;Zhang et al 1998;Murakami et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Factors for high frequency plant regeneration in tissue cultures of Indian mustard (Brassica juncea L.)

Bhulyan¹,

Min²,

Choi³

et al. 2009

Journal of Plant Biotechnology

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An efficient system for high frequency plant regeneration was established through investigating various factors such as plant growth regulator combinations, explant types and ages, and addition of AgNO3 influenced on shoot regeneration in Brassica juncea L. cv. BARI sarisha-10. Murashige and Skoog (MS) medium supplemented with 0.1 mg/L NAA (1-naphthaleneacetic acid) and 1 mg/L BA (6-benzyladenine) showed the maximum shoot regeneration frequency (56.67%) among the different combinations of NAA and BA. Explant type, explant age, and addition of AgNO3 also significantly affected shoot regeneration. Of the four type of explants (cotyledon, hypocotyl, root, and leaf explants)-cotyledon explants produced the highest shoot regeneration frequency and hypocotyls explants produced the highest number of shoots per explant, whereas root explants did not produce any shoot. The cotyledonary explants from Four-day-old seedlings showed the maximum shoot regeneration frequency and number of shoots per explant. Shoot regeneration frequency increased significantly by adding AgNO3 to the medium. Two mg/L AgNO3 appeared to be the best for shoot regeneration with the highest shoot regeneration frequency (86.67%) and number of shoots per explant (7.5 shoots). Considerable variation in shoot regeneration from cotyledonay explants was observed within the B. juncea L. genotypes. The shoot regeneration frequency ranged from 47.78% for cv. Shambol to 91.11% for cv. Rai-5. In terms of the number of shoots produced per explant, B. juncea L. cv. Daulot showed the maximum efficiency. MS medium supplemented with 0.1 mg/L NAA showed the highest frequency of rooting. The regenerated plantlets were transferred to pot soil and grown to maturity in the greenhouse. All plants were fertile and morphologically identical with the source plants.

show abstract

Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage ( Brassica campestris L. ssp. pekinensis )

Cited by 75 publications

References 20 publications

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

High frequency organogenesis in hypocotyl, cotyledon, leaf and petiole explants of broccoli (Brassica oleracea L. var. italica), an important vegetable crop

Characterization of a Collection of Brassica carinata Genotypes for in vitro Culture Response and Mode of Shoot Regeneration

Factors for high frequency plant regeneration in tissue cultures of Indian mustard (Brassica juncea L.)

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